Heterogeneity and 5′-terminal structures of the late RNAs of simian virus 40

Ghosh, Prabhat K.; Reddy, Vemuri B.; Swinscoe, J.; Lebowitz, Paul; Weissman, Sherman M.

doi:10.1016/0022-2836(78)90022-0

Cited by 371 publications

(217 citation statements)

References 45 publications

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“…Total cellular RNA was prepared from rat embryonic fibroblasts and adult rat stomach, uterus, vas deferens, heart, and skeletal muscle (thigh) as described previously (12,29 Primer extension analysis. Primer extension analysis was carried out essentially as previously described (32). For primer extension analysis, a 49-bp fragment corresponding to a 5'-untranslated region of the mRNA was obtained starting from amino acid 1 of exon 1 (see Fig.…”

Section: Methodsmentioning

confidence: 99%

Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

Helfman¹,

Cheley²,

Kuismanen³

et al. 1986

Mol. Cell. Biol.

175

122

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The molecular basis for the expression of rat embryonic fibroblast tropomyosin 1 and skeletal muscle beta-tropomyosin was determined. cDNA clones encoding these tropomyosin isoforms exhibit complete identity except for two carboxy-proximal regions (amino acids 189 to 213 and 258 to 284) and different 3'-untranslated sequences. The isoform-specific regions delineate the troponin T-binding domains of skeletal muscle tropomyosin. Analysis of genomic clones indicates that there are two separate loci in the rat genome that contain sequences complementary to these mRNAs. One locus is a pseudogene. The other locus contains a single gene made up of 11 exons and spans approximately 10 kilobases. Sequences common to all mRNAs were found in exons 1 through 5 (amino acids 1 to 188) and exons 8 and 9 (amino acids 214 to 257). Exons 6 and 11 are specific for fibroblast mRNA (amino acids 189 to 213 and 258 to 284, respectively), while exons 7 and 10 are specific for skeletal muscle mRNA (amino acids 189 to 213 and 258 to 284, respectively). In addition, exons 10 and 11 each contain the entire 3'-untranslated sequences of the respective mRNAs including the polyadenylation site. Although the gene is also expressed in smooth muscle (stomach, uterus, and vas deferens), only the fibroblast-type splice products can be detected in these tissues. S1 and primer extension analyses indicate that all mRNAs expressed from this gene are transcribed from a single promoter. The promoter was found to contain G-C-rich sequences, a TATA-like sequence TTTTA, no identifiable CCAAT box, and two putative Sp1-binding sites.

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Section: Methodsmentioning

confidence: 99%

Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

Helfman¹,

Cheley²,

Kuismanen³

et al. 1986

Mol. Cell. Biol.

175

122

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“…24 and references therein). In contrast, mRNAs coding for the structural proteins of SV40 and polyoma virus have heterogeneous 5' ends (15,25,26); however, A+T-rich sequences are not found 25 nucleotides upstream from these termini. Our observation of heterogeneity in the major 5' termini of early 104 Biochemistry: Ghosh et al…”

Section: Gelmentioning

confidence: 95%

“…The human line SV80, transformed by wild-type SV40, and rat fibroblasts, transformed by wild-type SV40 and the origin-defective mutants, were propagated as described (12). Vero African green monkey kidney cells were infected with wild-type SV40 and d1892 at a multiplicity of infection of [10][11][12][13][14][15][16][17][18][19][20] -4xn~Um c ci I 41= (2) AA GAGGC (3) CAGGC (3) GwkGC ( A/HindIII, spanning positions 5136-5171. Size analyses of cDNAs were carried out on thin (0.5-mm) polyacrylamide gels and sequence analyses by the method of Maxam and Gilbert (17).…”

mentioning

confidence: 99%

Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.

Ghosh

Lebowitz

Frisque³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

149

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“…The major 5'-terminal cap structure of wild-type SV40 late mRNA exists both in a cap 1 and a cap 2 form [14] and was accurately located at 0.722 map unit [15]. By reverse transcription of SV40-specific, cytoplasmic mRNA synthesized late during lytic infection, Reddy and coworkers [16] and Ghosh and coworkers [17] have found that both the 16-S and 19-S late mRNA species can be grouped into several categories depending on the structure of the leader fragment. The body of 1 6 4 and 19-S mRNA is coded by the DNA segment from 0.939 to 0.170 map unit and from 0.765 to 0.170 map unit, respectively, but the 5' termini of the multiple different leader fragments are distributed throughout the Hind-C sequence.…”

mentioning

confidence: 99%

Nucleotide Sequence of the Hind-C Fragment of Simian Virus 40 DNA. Comparison of the 5'-Untranslated Region of Wild-Type Virus and of Some Deletion Mutants

1979

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We report here the nucleotide sequence of the wild-type simian virus 40 (strain 776) r c~t t i~i i o n fragment Hind-C-PI DNA and of the homologous region of various mutant DNAs which lack part of this fragment. During this work, we detected between EcoRII fragments N and G an additional, 17-base-pair EcoRII fragment, fragment P, which had previously been overlooked. Also, an additional dTpdG dinucleotide at residues L 339 -340 was observed by sequence analysis of the DNA minus (E) strand; the presence of this dinucleotide was masked on sequencing patterns of the plus strand due to the persistence (during gel electrophoresis) of some secondary structures in the strand's 5'-terminal region. These nucleotide additions raise the total length of SV40 DNA to 5243 base pairs. The longest tandemly repeated segment in SV40 DNA now extends over 72 base pairs.SV40 deletion mutants dl 893 and dl 894 and SV40 strains Rh 91 1 and 1802 all lack an identical 72-base-pair-long DNA segment in the Hind-C region. This deletion corresponds precisely to one of the two aforementioned large tandemly repeated sequences. Mutant dl 895 lacks 66 base pairs, 63 of which are part of the former repetition. All these mutants, except dl 895, very probably were generated by an intramolecular, homologous recombination event. The 40-base-pair deletion in mutant dl 1811 includes the major capping site of SV40 late RNA. dl 1812 lacks only three base pairs, which are part of the overlapping HhaI and HpaII restriction sites at position 0.725 -0.726.Simian virus 40 (SV40) DNA replication starts at a unique position on the viral DNA, proceeds bidirectionally, and stops when the two replicating forks meet at a point exactly halfway around the circular genome [2-41. The origin of replication was mapped both by pulse-labeling studies [5] and by electron microscopic analysis [6] around 0.67 map unit, i.e. within HindII + I11 restriction fragment C,

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Heterogeneity and 5′-terminal structures of the late RNAs of simian virus 40

Cited by 371 publications

References 45 publications

Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

Nonmuscle and muscle tropomyosin isoforms are expressed from a single gene by alternative RNA splicing and polyadenylation.

Identification of a promoter component involved in positioning the 5' termini of simian virus 40 early mRNAs.

Nucleotide Sequence of the Hind-C Fragment of Simian Virus 40 DNA. Comparison of the 5'-Untranslated Region of Wild-Type Virus and of Some Deletion Mutants

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