Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

Costa, Leandro Silva; Telles, Cinthia Beatrice Silva; Oliveira, R. M.; Nobre, Leonardo Thiago Duarte Barreto; Dantas-Santos, Nednaldo; Câmara, Rafael Barros Gomes; Costa, Mariana Santana Santos Pereira; Almeida-Lima, Jailma; Melo-Silveira, Raniere Fagundes; Albuquerque, Ivan Rui Lopes; Leite, Edda Lisboa; Rocha, Hugo Alexandre Oliveira

doi:10.3390/md9040603

Cited by 43 publications

(15 citation statements)

References 24 publications

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“…In another study, Costa et al [9] treated HeLa cells with crude fucoidan from S. filipendula for 24 h and found increased Annexin V expression, and the authors suggest that the cells underwent apoptosis via (glycogen synthase kinase-beta) GSK3β activation despite also reporting that there were no changes in caspase 9, caspase 3, p53, NFκB, ERK or p38 expression. Activated GSK-3β interacts with p53 to initiate the caspase cascade [78] and/or NFκB to activate the TNF signalling cascade [79,80] in order to induce apoptosis [81].…”

Section: S Filipendula Fucoidan Causes Necrosis In Mg63 Cellsmentioning

confidence: 99%

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

et al. 2020

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Fucoidan is a brown algae-derived polysaccharide having several biomedical applications. This study simultaneously compares the anti-cancer activities of crude fucoidans from Fucus vesiculosus and Sargassum filipendula, and effects of low (LMW, 10–50 kDa), medium (MMW, 50–100 kDa) and high (HMW, >100 kDa) molecular weight fractions of S. filipendula fucoidan against osteosarcoma cells. Glucose, fucose and acid levels were lower and sulphation was higher in F. vesiculosus crude fucoidan compared to S. filipendula crude fucoidan. MMW had the highest levels of sugars, acids and sulphation among molecular weight fractions. There was a dose-dependent drop in focal adhesion formation and proliferation of cells for all fucoidan-types, but F. vesiculosus fucoidan and HMW had the strongest effects. G1-phase arrest was induced by F. vesiculosus fucoidan, MMW and HMW, however F. vesiculosus fucoidan treatment also caused accumulation in the sub-G1-phase. Mitochondrial damage occurred for all fucoidan-types, however F. vesiculosus fucoidan led to mitochondrial fragmentation. Annexin V/PI, TUNEL and cytochrome c staining confirmed stress-induced apoptosis-like cell death for F. vesiculosus fucoidan and features of stress-induced necrosis-like cell death for S. filipendula fucoidans. There was also variation in penetrability of different fucoidans inside the cell. These differences in anti-cancer activity of fucoidans are applicable for osteosarcoma treatment.

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Section: S Filipendula Fucoidan Causes Necrosis In Mg63 Cellsmentioning

confidence: 99%

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

et al. 2020

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“…Sulfate content was determined according to the gelatin-barium method as previously described [ 18 ], using sodium sulfate (1 mg/mL) as a standard and following acid hydrolysis of the polysaccharides (4 M HCl, 100°C, 6 h). Protein content was measured using the Spector's method [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

In VivoEvaluation of the Antioxidant Activity and Protective Action of the SeaweedGracilaria birdiae

Barros-Gomes

Nascimento

Silveira

et al. 2018

Oxidative Medicine and Cellular Longevity

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The red seaweed Gracilaria birdiae (GB) is farmed and used as food in northeast Brazil. However, the economic potential of this seaweed has been explored little. To enable direct consumption and/or product diversification from GB, it is necessary to evaluate its effect in vivo. In this study, the food of mice was improved with the addition of GB. After 21 days, the consumption of seaweed reduced the weight gain and blood glucose levels in mice. In addition, it increased the trolox equivalent antioxidant capacity and glutathione reductase and catalase levels compared to those of the control group. In addition, some mice also received carbon tetrachloride (CCl4). In this case, histological, enzymatic, and antioxidant tests showed that the seaweed could protect animals from damage caused by this toxic agent. In addition, GB aqueous extract (AE) inhibited 50% of 3T3-L1 cell differentiation into adipocytes, whereas GB ethanolic extract was not effective. AE is composed mainly of sulfated polysaccharides. The results of the present study indicate that the alga GB protected the mice from CCl4-induced damage, indicating that the seaweed exhibits protective action in vivo. In addition, GB decreased the animal weight gain, which was mainly due to the action of the sulfated polysaccharides synthesized by this seaweed.

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“…Apoptosis measurement was carried out mainly according to the literature [ 1 ] with some necessary variations. HeLa cells were treated with P2 at indicated concentrations for 12, 24, and 48 h. Then they were harvested (including attached and detached cells), washed, and resuspended with PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Cervical cancer, the second most common type of tumor in females surpassed only by breast cancer [ 1 ], becomes a major health problem worldwide despite advances in screening [ 2 ]. For early-stage cervical cancer, patients are primarily treated by surgery or a combination of radiotherapy/chemotherapy [ 1 ]. Chemotherapeutic drugs are limited in clinical use because of their serious adverse effects, particularly with increased doses and prolonged courses [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Polypeptide Fraction fromArca subcrenataInduces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways

Zhang

Liu

et al. 2015

Evidence-Based Complementary and Alternative Medicine

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Arca subcrenata is documented in the literature of marine Traditional Chinese Medicine. Polypeptide fraction from A. subcrenata, coded as P2, was demonstrated to possess significant antitumor activity in our previous study. However, the underlying mechanism remains undefined. The present study was carried out to investigate the underlying antitumor mechanism of P2 in human cervical cancer HeLa cells by MTT, FCM, LSCM, and western blot assays. The results revealed that P2 significantly induced apoptosis of HeLa cells in a concentration- and time-dependent manner. High level of ROS was provoked by P2, which was in turn responsible for induction of apoptosis through activation of intrinsic mitochondrial pathway and JNK1/2, p38 MAPK pathways, as well as inhibition of ERK1/2 pathway, as evidenced by the abrogation of P2's effect on HeLa cells preincubated with the ROS scavenger NAC. P2 also was observed to display significant effect on G2/M phase arrest by downregulating the expression of cyclin B1/cdc2 complex and upregulating the expression of p21. These findings demonstrate that P2 induces apoptosis and G2/M phase arrest in HeLa cells through ROS-mediated MAPKs pathways, suggesting that P2 would be worth investigating as a promising agent within the scope of marine drugs for treatment of cervical cancer.

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Heterofucan from Sargassum filipendula Induces Apoptosis in HeLa Cells

Cited by 43 publications

References 24 publications

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

Fucoidan Inhibition of Osteosarcoma Cells is Species and Molecular Weight Dependent

In VivoEvaluation of the Antioxidant Activity and Protective Action of the SeaweedGracilaria birdiae

Polypeptide Fraction fromArca subcrenataInduces Apoptosis and G2/M Phase Arrest in HeLa Cells via ROS-Mediated MAPKs Pathways

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