Heteroduplex Mapping of Heat-Resistant Deletion Mutants of Bacteriophage T5

Scheible, Patricia P.; Rhoades, Marc

doi:10.1128/jvi.15.5.1276-1280.1975

Cited by 22 publications

(23 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results presented here demonstrate that BF23 DNA can be deleted, without affecting viability in common laboratory hosts, in a region different from that reported for viable deletion mutants of T5 (25). Viable deletion mutants of T5 have all involved the region from 0.216 to 0.324 of the fractional length from the left end of the T5 DNA molecule, and, as expected from the close relationship between BF23 and T5, we have shown here that viable deletion mutants of BF23 may also involve this same region.…”

Section: Discussioncontrasting

confidence: 71%

“…6a). The region deleted in BF23st(9) overlaps the deletable region in T5(+) DNA, which is between 0.22 and 0.32 of the fractional length from its left end (25), and includes the nick at position 0.33 of BF23(+) DNA. These findings confirm our previous conclusion that the nick at position 0.33 is deleted in BF23st(9) DNA and support our model for BF23st(4) and BF23st(9) DNA presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Up to 10% of the DNA of T5(+) can be deleted from a region of the DNA between 0.216 and 0.324 of the fractional length of the molecule measured from the left end (see Fig. 4) without affecting its viability in common laboratory hosts (25). Such deletion mutants are termed st mutants because they are more stable at elevated temperatures in salinecitrate buffer than is T5(+) (1, 3,10).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Terminally redundant deletion mutants of bacteriophage BF23

Shaw¹,

Lang²,

McCorquodale³

1979

J Virol

View full text Add to dashboard Cite

Deletion mutants of bacteriophage BF23 were isolated and the positions of the deletions were determined. Two different deletable regions were detected: one in the same region as previously reported for bacteriophage T5, which is closely related to BF23; and the other within both terminal repetitions. The former deletable region lay between positions 0.31 and 0.36, which represented the fractional lengths of the BF23(+) DNA as measured from its left end. The latter deletion was evenly divided between the two terminal repetitions. The deletion in the left terminal repetition lay between positions 0.044 and 0.078 and was repeated in the corresponding region of the right terminal repetition between positions 0.966 and 1.0. The size of the DNA transferred to host cells during the first step of DNA transfer by BF23 carrying deletions in the terminal repetitions of its DNA was less than the size of DNA transferred during the first step by wild-type BF23 by an amount equal to the size of the deletion in each terminal repetition. This finding suggests the existence of a specific mechanism for delineating the position at which the first step of DNA transfer is stopped.

show abstract

Section: Discussioncontrasting

confidence: 71%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Terminally redundant deletion mutants of bacteriophage BF23

Shaw¹,

Lang²,

McCorquodale³

1979

J Virol

View full text Add to dashboard Cite

show abstract

“…Included in this group are fragments 18 through 33 and possibly some of the smaller fragments. Fragment II also contains a strong band between fragments 22 and 24, which represents the interval between the prominent interruption at 18.5% and the EcoRI cleavage point at 25.0% (Fig. 4).…”

Section: -mentioning

confidence: 99%

“…They represent shortened versions of fragments 7 and 13. genome. T5st(124) DNA lacks a 4.5% segment that maps entirely to the left of the 32.6% interruption (22). In this DNA, fragment 13 should be replaced with a new 9.6% fragment.…”

Section: -mentioning

confidence: 99%

Localization of Single-Chain Interruptions in Bacteriophage T5 DNA. II. Electrophoretic Studies

Rhoades

1977

J Virol

Self Cite

View full text Add to dashboard Cite

Upon denaturation, T5 DNA yields a large number of discrete, single-chain fragments that can be resolved by agarose gel electrophoresis. The positions of the more prominent of these fragments in the T5 duplex were determined by analyzing their sensitivity to digestion with X exonuclease and their distribution among EcoRI fragments of T5 DNA. These experiments also provide firm evidence concerning the polarity of the strands in T5 DNA. An analogous study was carried out on the fragments produced by treating exonuclease III-degraded T5 DNA with the single-strand-specific SI endonuclease. This procedure yielded over 40 discrete duplex fragments that could be resolved with considerable precision by agarose gel electrophoresis. The positions of most of these fragments were determined by analyzing EcoRI fragments of T5st(+) and T5st(0) DNA. Over 20 sites where single-chain interruptions can occur in T5 DNA were identified, and the distribution of interruptions within the terminal repetition was shown to be identical at both ends of the molecule. A precise value for the size of the terminal repetition in T5 DNA was obtained by analyzing SI endonuclease digests of ligase-repaired, circular T5 DNA in agarose gels. The repeated segment represented 8.3% of the T5st(+) DNA. The results of this study also provide information concerning the properties of X exonuclease. Hydrolysis by this enzyme was not terminated when single-chain interruptions were encountered either in the strand being degraded or in the complementary strand.

show abstract

Reproduction of Large Virulent Bacteriophages

Mathews¹

1977

Comprehensive Virology

View full text Add to dashboard Cite

Heteroduplex Mapping of Heat-Resistant Deletion Mutants of Bacteriophage T5

Cited by 22 publications

References 19 publications

Terminally redundant deletion mutants of bacteriophage BF23

Terminally redundant deletion mutants of bacteriophage BF23

Localization of Single-Chain Interruptions in Bacteriophage T5 DNA. II. Electrophoretic Studies

Reproduction of Large Virulent Bacteriophages

Contact Info

Product

Resources

About