Heterochromatin and rDNA 5S and 45S sites as reliable cytogenetic markers for castor bean (Ricinus communis, Euphorbiaceae)

Vasconcelos, Santelmo; Souza, Analice Araújo de; Gusmão, Cássia Lima Silva; Milani, Máira; Benko‐Iseppon, Ana Maria; Brasileiro-Vidal, Ana Christina

doi:10.1016/j.micron.2010.06.002

Cited by 21 publications

(21 citation statements)

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“…Cytogenetic studies such as karyotyping and fluorescence in situ hybridization (FISH) are indispensable to basic studies for improvement of economically important plant species (Sadder and Webber, 2001;Vasconcelos et al, 2010). The advancement of molecular cytogenetic techniques has made chromosome characterization more efficient and reliable (Wiegant et al, 1991;Dong et al, 2000;Levsky and Singer, 2003;Fukui, 2005).…”

Section: Introductionmentioning

confidence: 99%

Dual-color FISH karyotype analyses using rDNAs in three Cucurbitaceae species

Waminal

Kim

2011

Genes Genom

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Dual-color fluorescence in situ hybridization (FISH) analysis of three Cucurbitaceae species from different genera was conducted using 5S and 45S rDNA probes. In Benincasa hispida (Thunb.) Cogn. (2n=24), the 45S rDNA probe hybridized on two chromosomes, one in the short arm of a medium-sized metacentric chromosome and another at the satellite of a chromosome. The 5S rDNA hybridized at a site proximal to the centromere of the same short arm of the 45S rRNA gene locus that occupied almost the entire short arm. For Citrullus lanatus (Thunb.) Matsum & Nakai (2n=22), the 45S rDNA probe hybridized at sites in the short arms of two chromosomes and the 5S rDNA probe was co-localized with the 45S rRNA locus at the region proximal to the centromere in one chromosome. The 45S rRNA loci occupied almost all of the short arms in both chromosomes. In Cucurbita moschata Duch. (2n=40), the 45S rDNA probe hybridized in five chromosomes in which the 45S rRNA genes occupied almost two-thirds of the chromosomes in two large chromosomes and the entire short arm of a medium-sized chromosome. Two other loci were present in two medium-sized chromosomes, one in the proximal region in the short arm of a chromosome and another at the tip of the long arm of a chromosome. Chromosomes of B. hispida were relatively larger than those of the other two species. The karyotype of B. hispida is composed of two metacentrics and 10 submetacentrics, while that of C. lanatus is composed of seven metacentrics and four submetacentrics and that of C. moschata is composed of 18 metacentrics and two submetacentrics. Comparative chromosome evolution among the three Cucurbitaceae species was attempted using the karyotypes and the chromosomal distribution patterns of the 5S and 45S rDNAs. The results presented herein will be useful in elucidating the phylogenetic relationships among Cucurbitaceae species, and will provide basic data for their breeding programs.

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Section: Introductionmentioning

confidence: 99%

Dual-color FISH karyotype analyses using rDNAs in three Cucurbitaceae species

Waminal

Kim

2011

Genes Genom

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“…100 cells of each species (with a Leica DMLB epifluorescence microscope and an Olympus SP350 camera), slides were destained in ethanol:acetic acid (3:1, v/v) for 30 min, followed by immersion in ethanol for 1 h. Then, chromosome preparations were hydrolyzed in 5 N HCl at room temperature for 20 min and stained with 2 % Giemsa for 15 min and mounted with Entellan (Merck), for further image acquisition. Silver nitrate (AgNO 3 ) impregnation of interphase nuclei followed the protocol described by Hizume et al (1980), with the modifications described in Vasconcelos et al (2010). Thirty microliters of 50% silver nitrate were added to the preparations, which were covered with a piece of nylon mesh (24×32 cm), incubated at 37 °C for 40 min in a moisture chamber, being subsequently washed in distilled water and mounted with Entellan.…”

Section: Cytogenetic Analysesmentioning

confidence: 99%

Evidence of genetic differentiation and karyotype evolution of the sedges Cyperus ligularis L. and C. odoratus L. (Cyperaceae)

et al. 2018

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Th e taxonomy of Cyperaceae is complex, with genera like Cyperus harboring species complexes. We analyzed the genetic similarity between Cyperus ligularis L. and C. odoratus L. based on DNA fi ngerprinting and cytogenetics. Signifi cative genetic diff erentiation (G ST = 0.363) and low gene fl ow (N m = 0.877) indicated a clear genetic distinction between the two species. Moreover, the clustering analysis showed two distinct genetic groups, suggesting a lack of evidence for hybridization. Th e phenogram revealed two diff erent lineages, and although all individuals of C. odoratus were collected from plots close to each other, they possessed greater genetic diversity than that observed among individuals of C. ligularis, which were sampled over a wider geographic range. Variation in chromosome number within the two species exhibited the opposite pattern, indicating greater karyotype stability in C. odoratus with 2n = 72 and 2n = 76, while the diploid number for C. ligularis varied from 2n = 66 to 88. Th e lower genetic variation in C. ligularis may be a result of the founder eff ect associated with seed dispersion and clonal reproduction. Field observations and analysis of reproductive biology should enrich the understanding of the genetic structure of the investigated populations and their role in successional processes.

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“…Cuadrado & Jouve (2007), for instance, analyzing the distribution pattern of trinucleotide repeats in barley (Hordeum vulgare) chromosomes by means of FISH, observed a preference of these large microsatellite clusters for heterochromatic regions, except for the ACT-based probe. In relation to the distribution of heterochromatin through castor bean chromosomes, large heterochromatic blocks have been related, including all pericentromeric regions (Jelenkovic & Harrington, 1973;Paris et al, 1978;Vasconcelos et al, 2010), in which the SSR sequences may be important constituents. Thus, in the present work an in situ hybridization was performed with the synthetic oligonucleotide (TGA) 6 as probe, aiming to test the potentiality of using large microsatellite clusters to characterize castor bean accessions.…”

Section: Genome Sequencing and The Use Of Co-dominant Markersmentioning

confidence: 99%

“…Thus, in the present work an in situ hybridization was performed with the synthetic oligonucleotide (TGA) 6 as probe, aiming to test the potentiality of using large microsatellite clusters to characterize castor bean accessions. Cell preparations, image documentation and FISH conditions followed Vasconcelos et al (2010); probe preparation was done according to Cuadrado & Jouve (2007).…”

Section: Genome Sequencing and The Use Of Co-dominant Markersmentioning

confidence: 99%