Heterobasidion wood decay fungi host diverse and globally distributed viruses related to Helicobasidium mompa partitivirus V70

Kashif, Muhammad; Hyder, Rafiqul; Pérez, Dafer; Hantula, Jarkko; Vainio, Eeva J.

doi:10.1016/j.virusres.2014.09.002

Cited by 24 publications

(30 citation statements)

References 36 publications

(60 reference statements)

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“…Phenotype of H. annosum 94233 with and without HetPV13-an1. H. annosum 94233, the natural host of HetPV13-an1 (20), has a low growth rate and displays abnormal mycelial morphology characterized by increased hyphal branching ( Fig. 1).…”

Section: Resultsmentioning

confidence: 99%

“…1). The absence of HetPV13-an1 in the cured isolate was verified by reverse transcription-PCR (RT-PCR) with specific primers PV13An1midFor2 and PV13midR (20) using four independent cDNA samples. Moreover, the introduction of HetPV13-an1 back to this cured strain regenerated the debilitated phenotype (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…1C). The subcultures were verified to be HetPV13-an1 positive based on RT-PCR with specific primers (20). The isogenic strains could be differentiated based on their original growth pattern on the agar plate (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…HetPV13 strains closely similar to HetPV13-an1 occur naturally in H. annosum and H. parviporum (20,42), but these strains do not cause significant levels of growth debilitation on artificial culturing media. Future studies are needed to examine which specific sequence polymorphisms between the virus strains have a contribution to host effects and to investigate these effects in natural conditions.…”

Section: Discussionmentioning

confidence: 98%

“…Heterobasidion species host members of Partitiviridae (14)(15)(16)(17)(18)(19)(20), Narnaviridae (21), and the unassigned taxon Heterobasidion RNA virus 6 (HetRV6) (22). Members of the virus family Partitiviridae have bipartite double-stranded RNA (dsRNA) genomes that infect plants, fungi, or protozoa (23).…”

mentioning

confidence: 99%

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Heterobasidion Partitivirus 13 Mediates Severe Growth Debilitation and Major Alterations in the Gene Expression of a Fungal Forest Pathogen

Vainio

Jurvansuu

Hyder

et al. 2018

J Virol

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The fungal genus includes some of the most devastating conifer pathogens in the boreal forest region. In this study, we showed that the alphapartitivirus Heterobasidion partitivirus 13 from (HetPV13-an1) is the main causal agent of severe phenotypic debilitation in the host fungus. Based on RNA sequencing using isogenic virus-infected and cured fungal strains, HetPV13-an1 affected the transcription of 683 genes, of which 60% were downregulated and 40% upregulated. Alterations observed in carbohydrate and amino acid metabolism suggest that the virus causes a state of starvation, which is compensated for by alternative synthesis routes. We used dual cultures to transmit HetPV13-an1 into new strains of and The three strains of that acquired the virus showed noticeable growth reduction on rich culturing medium, while only two of six isolates tested showed significant debilitation. Based on reverse transcription-quantitative PCR (RT-qPCR) analysis, the response toward HetPV13-an1 infection was somewhat different in and We assessed the effects of HetPV13-an1 on the wood colonization efficacy of in a field experiment where 46 Norway spruce trees were inoculated with isogenic strains with or without the virus. The virus-infected strain showed considerably less growth within living trees than the isolate without HetPV13-an1, indicating that the virus also causes growth debilitation in natural substrates. A biocontrol method restricting the spread of species would be highly beneficial to forestry, as these fungi are difficult to eradicate from diseased forest stands and cause approximate annual losses of €800 million in Europe. We used virus curing and reintroduction experiments and RNA sequencing to show that the alphapartitivirus HetPV13-an1 affects many basic cellular functions of the white rot wood decay fungus, which results in aberrant hyphal morphology and a low growth rate. Dual fungal cultures were used to introduce HetPV13-an1 into a new host species, , and field experiments confirmed the capability of the virus to reduce the growth of in living spruce wood. Taken together, our results suggest that HetPV13-an1 shows potential for the development of a future biocontrol agent against fungi.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 98%

mentioning

confidence: 99%

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Heterobasidion Partitivirus 13 Mediates Severe Growth Debilitation and Major Alterations in the Gene Expression of a Fungal Forest Pathogen

Vainio

Jurvansuu

Hyder

et al. 2018

J Virol

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Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland

et al. 2017

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We investigated the diversity and spatial distribution of viruses infecting strains of the root rot fungus Heterobasidion annosum collected from pine stumps at a heavily infected forest site. Four different partitiviruses were detected in 14 H. annosum isolates at the study site, constituting approximately 29% of all Heterobasidion isolates investigated (N = 48). Two of the viruses detected were new partitiviruses designated here as Heterobasidion partitivirus 16 (HetPV16) and HetPV20, and two were previously known partitiviruses: HetPV7 and HetPV13. The two new partitiviruses found, HetPV16-an1 and HetPV20-an1, shared ~70% RdRp nucleotide sequence identity with the alphapartitivirus Rosellinia necatrix partitivirus 2, and less than 40% identity with known viruses of Heterobasidion spp. HetPV7-an1 was closely similar to HetPV7-pa1 isolated earlier from Heterobasidion parviporum, supporting the view of conspecific virus pools in different Heterobasidion species. Three fungal isolates were found to be co-infected with two different partitivirus strains (HetPV7-an1 and HetPV13-an2 or HetPV16-an1 and HetPV20-an1). Different isolates representing each host clone had variable virus compositions, and virus strains occurring in more than one host clone showed minor sequence variations between clones.

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Erratum to: Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland

et al. 2017

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Heterobasidion wood decay fungi host diverse and globally distributed viruses related to Helicobasidium mompa partitivirus V70

Cited by 24 publications

References 36 publications

Heterobasidion Partitivirus 13 Mediates Severe Growth Debilitation and Major Alterations in the Gene Expression of a Fungal Forest Pathogen

Heterobasidion Partitivirus 13 Mediates Severe Growth Debilitation and Major Alterations in the Gene Expression of a Fungal Forest Pathogen

Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland

Erratum to: Distribution of Viruses Inhabiting Heterobasidion annosum in a Pine-Dominated Forest Plot in Southern Finland

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