Hetero-fertilization together with failed egg–sperm cell fusion supports single fertilization involved in in vivo haploid induction in maize

Tian, Xiaolong; Qin, Yuanxin; Chen, Baojian; Liu, Chenxu; Wang, Lele; Li, Xingli; Dong, Xin; Liu, Liwei; Chen, Shaojiang

doi:10.1093/jxb/ery177

Cited by 33 publications

(30 citation statements)

References 36 publications

(76 reference statements)

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“…In conclusion, it is still unknown how haploid induction is mechanistically triggered upon pollination by Stock6-derived lines and mtl/pla1/nld mutants. Investigations to study both uniparental chromosome elimination (Li et al 2009 , 2017 ; Zhang et al 2008 ; Zhao et al 2013 ) and single fertilization (Tian et al 2018 ) are underway.…”

Section: Generation Of Haploids Via Intraspecific Hybridizationmentioning

confidence: 99%

State-of-the-art and novel developments of in vivo haploid technologies

et al. 2018

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The ability to generate (doubled) haploid plants significantly accelerates the crop breeding process. Haploids have been induced mainly through the generation of plants from cultivated gametophic (haploid) cells and tissues, i.e., in vitro haploid technologies, or through the selective loss of a parental chromosome set upon inter- or intraspecific hybridization. Here, we focus our review on the mechanisms responsible for the in vivo formation of haploids in the context of inter- and intraspecific hybridization. The application of a modified CENH3 for uniparental genome elimination, the IG1 system used for paternal as well as the BBM-like and the patatin-like phospholipase essential for maternal haploidy induction are discussed in detail.

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Section: Generation Of Haploids Via Intraspecific Hybridizationmentioning

confidence: 99%

State-of-the-art and novel developments of in vivo haploid technologies

et al. 2018

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“…According to the current study, the relationship between haploid induction and kernel abortion suggest that this kind of kernel defective is not only coming from the process of double fertilization, but may also resulting from the mechanism of haploid induction. Tian et al [1] observed ovules with an unfertilized egg cell and a fertilized central cell (endosperm) during the process of the haploid induction crosses, which provided direct evidence for the singlefertilization hypothesis and identifying the origins of defective kernels produced in vivo, it was inferred single fertilization and chromosome elimination may lead to formation of defective kernels caused by the inducer pollen. The majority of previous studies have mainly focused on the haploid induction rate improvement but ignored simultaneous effects occurring by haploid induction.…”

Section: Relationship Between Kernel Abortion and Inducibilitymentioning

confidence: 99%

“…Thus there is no or little starch accumulated in the endosperm, resulting in formation of membranoid substance or yellow granule, which is called abortion tablet. If flow interruption happens15-25 days after double fertilization, grain filling starts but stops halfway, leading to limited starch accumulation in the endosperm, and shrunken grains, causing a diapause tablet [1]. In order to determine, how these kernel defects happen, several mutants were studied and causative genes cloned, one example is defective kernel1 (dek1), required for aleurone cell development in the endosperm of maize grains, which encodes a membrane protein of the calpain gene superfamily [2,3,4].…”

Section: Introductionmentioning

confidence: 99%

“…The major gene responsible for haploid induction rate has been cloned by three independent groups and has been named matrilineal (MTL), ZmPHOSPHOLIPASE, A1, and NOT LIKE DAD (NLD), respectively [11,12,13]. Up to now, there are two ways both contributed to in vivo induced haploid [1]. Single fertilization as one possible mechanism [14,15] is defined as one of the two sperm cells failing to fuse with an egg cell but instead triggering haploid embryogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.)

Ren

et al. 2020

PLoS ONE

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Kernel abortion is common phenomenon in vivo haploid induction and closely linked with haploid induction rate, but little information of kernel abortion is available and its genetic basis still unclear. We used two mapping populations including 186 and 263 F 2.3 family lines to analyze the different degree of kernel abortion and identify quantitative trait loci (QTL) responsible for kernel abortion during haploid induction. In total 62 putative QTL, accounting for 3.27-14.70% of the phenotypic variation in kernel abortion traits, were detected across all 10 chromosomes. Ten QTL with over 10% contribution to phenotypic variation were affecting the fifth level of endosperm abortion (EnA5 th), endosperm abortion (EnA) and total abortion (TA). Co-localization among kernel abortion traits QTL was observed in both populations and among different kernel abortion types. Five overlaps were indentified in the QTL for kernel abortion traits and HIR traits. Maize chromosome bins 3.

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“…Recently, major breakthroughs have been made by cloning the large effect QTLs for haploid induction, i.e., qhir1 (Kelliher et al 2017;Gilles et al 2017;Liu et al 2017) and qhir8 (Zhong et al 2019). These cloned genes and associated molecular evidence allowed researchers to re-evaluate the two competing hypotheses explaining the maternal haploid induction: (1) regular double fertilization followed by male chromosome elimination and (2) impaired double fertilization or single fertilization (Li et al 2009a;Tian et al 2018). It eventually led to a unified hypothesis that both fertilization defects and chromosome elimination could be involved in the maternal haploid induction (Chaikam et al 2019b;Jacquier et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Genomic selection to optimize doubled haploid-based hybrid breeding in maize

Cheng

Guo

et al. 2020

Preprint

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Crop improvement, as a long-term endeavor, requires continuous innovations in technique from multiple perspectives. Doubled haploid (DH) technology for pure inbred production, which shaves years off of the conventional selfing approach, has been widely used for breeding. However, the final success rate of in vivo maternal DH production is determined by four factors: haploids induction, haploids identification, chromosome doubling, and successful selfing of the fertile haploid plants to produce DH seeds. Traits in each of these steps, if they can be accurately predicted using genomic selection methods, will help adjust the DH production protocol and simplify the logistics and save costs. Here, a hybrid population (N=158) was generated based on an incomplete half diallel design using 27 elite inbred lines. These hybrids were induced to create F1-derived haploid families. The hybrid materials, as well as the 27 inbreds, the inbred-derived haploids (N=200), and the F1-derived haploids (N=5,000) were planted in the field to collect four DH-production traits, three yield-related traits, and three developmental traits. Quantitative genetics analysis suggested that in both diploids and haploid families, most of the developmental traits showed high heritability, while the DH-production and developmental traits exhibited intermediate levels of heritability. By employing different genomic selection models, our results showed that the prediction accuracy ranged from 0.52 to 0.59 for the DH-production traits, 0.50 to 0.68 for the yield-related traits, and 0.44 to 0.87 for the developmental traits. Further analysis using index selection achieved the highest prediction accuracy when considering both DH production efficiency and the agronomic trait performance. Furthermore, the long-term responses through simulation confirmed that index selection would increase the genetic gain for targeted agronomic traits while maintaining the DH production efficiency. Therefore, our study provides an optimization strategy to integrate GS technology for DH-based hybrid breeding.

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Hetero-fertilization together with failed egg–sperm cell fusion supports single fertilization involved in in vivo haploid induction in maize

Abstract: Hetero-fertilization and failed egg–sperm cell fusion indicate that single fertilization plays a key role in in vivo haploid induction in maize.

Cited by 33 publications

References 36 publications

State-of-the-art and novel developments of in vivo haploid technologies

State-of-the-art and novel developments of in vivo haploid technologies

Mapping of QTL for kernel abortion caused by in vivo haploid induction in maize (Zea mays L.)

Genomic selection to optimize doubled haploid-based hybrid breeding in maize

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