Hessian fly resistance gene H13 is mapped to a distal cluster of resistance genes in chromosome 6DS of wheat

Abstract

H13 is inherited as a major dominant resistance gene in wheat. It was previously mapped to chromosome 6DL and expresses a high level of antibiosis against Hessian fly (Hf) [Mayetiola destructor (Say)] larvae. The objective of this study was to identify tightly linked molecular markers for marker-assisted selection in wheat breeding and as a starting point toward the map-based cloning of H13. Fifty-two chromosome 6D-specific microsatellite (simple sequence repeat) markers were tested for linkage to H13 using near-isogenic lines Molly (PI 562619) and Newton-207, and a segregating population consisting of 192 F(2:3) families derived from the cross PI 372129 (Dn4) x Molly (H13). Marker Xcfd132 co-segregated with H13, and several other markers were tightly linked to H13 in the distal region of wheat chromosome 6DS. Deletion analysis assigned H13 to a small region closely proximal to the breakpoint of del6DS-6 (FL 0.99). Further evaluation and comparison of the H13-linked markers revealed that the same chromosome region may also contain H23 in KS89WGRC03, an unnamed H gene (H(WGRC4)) in KS89WGRC04, the wheat curl mite resistance gene Cmc4, and a defense response gene Ppo for polyphenol oxidase. Thus, these genes comprise a cluster of arthropod resistance genes. Marker analysis also revealed that a very small intercalary chromosomal segment carrying H13 was transferred from the H13 donor parent to the wheat line Molly.

Identification of a novel gene, H34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers

“…In this study, we used a RIL population instead of F 2 as reported in most previous studies (Dweikat et al 2002;Martín-Sánchez et al 2003;Kong et al 2005Kong et al , 2008Liu et al 2005bLiu et al , 2005cYu et al 2009) to improve phenotyping accuracy. RILs have a high recombination frequency resulting from multiple meiotic events that occurred during repeated selfing (Jansen 2003), and a high level of homozygosity that enables replicated phenotyping across different environments.…”

“…Molecular markers closely linked to these genes are essential for such gene pyramiding, however, many earlier reported genes were located to chromosomes using monosomic analysis (Gallun and Patterson 1977;Ohm et al 1995). Some were mapped using DNA markers, but the mapping populations used were mainly F 2 generations (Williams et al 2003;Liu et al 2005a;Liu et al 2005c;Wang et al 2006;Kong et al 2008;Yu et al 2009;Miranda et al 2010). Because only a single plant was phenotyped without replication, escape of infestation may cause significant errors in phenotypic data.…”

Reactive Oxygen Species Are Involved in Plant Defense against a Gall Midge

“…Newton is the susceptible recurrent parent, whereas Iris and Molly carry R genes H9 and H13, respectively (Patterson et al, 1994). H9 and H13, located on different chromosomes (Liu et al, 2005a(Liu et al, , 2005b, are both major dominant genes that have a gene-for-gene interaction with Hessian fly avirulence. Rice is a nonhost of Hessian fly (Chen et al, 2009b).…”

Translocações cromossômicas entre trigo e centeio: uma alternativa ao melhoramento

“…Já o cromossomo 2R do centeio apresenta genes de resistência ao oídio e à ferrugem da folha e do colmo (BRUNELL et al, 1999), ao ataque da mosca Hessian (LIU et al, 2005;SARDESAI et al, 2005;ZHAO et al, 2006) e ao nanismo amarelo da cevada (barley yellow dwarf virus -BYDV) (NKONGOLO & COMEAU, 1998).…”

“…Vários genes de resistência à mosca Hessian (Mayetiola destrutor) foram mapeados no cromossomo do centeio 2RL, dentre eles H13, (LIU et al, 2005), H32 (SARDESAI et al, 2005) e H22 (ZHAO et al, 2006). HATCHETT et al (1993), por exemplo, estudaram o efeito da translocação 2BS.2RL na cultivar 'Hamlet' e verificaram que esta confere resistência ao biotipo L da mosca Hessian, o mais virulento dentre eles.…”