Hesperidin protects against IL‑1β‑induced inflammation in human osteoarthritis chondrocytes

Abstract

Hesperidin is a vitamin P flavonoid compound primarily present in citrus fruits, which possesses an anti-inflammatory effect. The functional role of hesperidin in interleukin (IL)-1β-stimulated human osteoarthritis (OA) chondrocytes is still unknown. In the present study, anti-inflammatory effects of hesperidin in IL-1β-stimulated chondrocytes were investigated. The results demonstrated that hesperidin treatment markedly decreased nitric oxide and prostaglandin E2 production and markedly downregulated inducible nitric oxide synthase and cyclooxygenase-2 expression in IL-1β-stimulated OA chondrocytes. In addition, hesperidin markedly reduced IL-1β-induced matrix metalloproteinase (MMP)-3 and MMP-13 expression in human OA chondrocytes. Furthermore, hesperidin markedly decreased the activation of nuclear factor (NF)-κB in human OA chondrocytes. In conclusion, it was revealed for the first time that hesperidin inhibited inflammatory responses in IL-1β-stimulated human chondrocytes, potentially through inhibiting the activation of the NF-κB signaling pathway. These data suggest that hesperidin may be a potential agent for the treatment of OA.

Physicochemical characterization of ‘Cerrado’ cashew (Anacardium othonianum Rizzini) fruits and pseudofruits

“…Hesperidin possesses vitamin‐like activity and can decrease capillary permeability (vitamin P), leakiness and fragility . It also showed anti‐oxidant, anti‐inflammatory, anti‐carcinogenic and anti‐allergic properties …”

“…32 It also showed anti-oxidant, anti-inflammatory, anti-carcinogenic and anti-allergic properties. [32][33][34] wileyonlinelibrary.com/jsfa The third and fourth most common compounds in the accessions studied were epicatechin (34% of accessions) and kaempferol 3-O-glucoside (30% of accessions). High levels of epicatechin were found in accession #115 (0.055 g kg −1 ) whereas kaempferol-3-O-glucoside was found in accessions #4, #24 #56, #102 #114 and #146 (0.013 to 0.034 g kg −1 ).…”

Transient receptor potential ankyrin 1 (trpa1) mediates il-1β-induced apoptosis in rat chondrocytes via calcium overload and mitochondrial dysfunction

“…It is noteworthy that TRPA1 can be activated by not only numerous exogenous pungent compounds but also agents formed endogenously in inflammatory reactions, such as nitric oxide [31], prostaglandins [32], and reactive oxygen species [33]. Moreover, in OA chondrocytes, the IL-1β has the ability to stimulate the production of mediators including nitric oxide [34], prostaglandin [35], and reactive oxygen species [36], which are involved in TRPA1 channel activation. Therefore, in these inflammatory cells, IL-1β maybe firstly upregulated the gene and protein expression of TRPA1, and then the increased expression of TRPA1 channel was activated by the endogenous agonists formed in inflammatory reactions, such as nitric oxide, prostaglandins, or reactive oxygen species.…”

Is hesperidin essential for prophylaxis and treatment of COVID-19 Infection?

“…Hesperidin with its high anti-inflammatory activity inhibited the secretion of pro-inflammatory cytokines such as IFN-γ and IL-2 [17]. Besides, hesperidin inhibited IL-1β-stimulated inflammatory responses by inhibiting the activation of the NF-κB signaling cascade [18]. It also played a major rule in suppressing the release of inflammatory markers such as (TNFα and IL-6) in type 2 diabetic patients [19].…”