Hesperidin-mediated expression of Nrf2 and upregulation of antioxidant status in senescent rat heart

Elavarasan, Jayasudha; Velusamy, Prema; Ganesan, Thellamudhu; Ramakrishnan, Suresh Krishna; Rajasekaran, Devaraja; Kalaiselvi, Periandavan

doi:10.1111/j.2042-7158.2012.01512.x

Cited by 103 publications

(64 citation statements)

References 58 publications

(87 reference statements)

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“…This result suggests that flavanones of the Citrus genus could have an important impact on the improvement of endogenous anti-oxidant defences in dyslipidaemia. Very recently, hesperidin has been demonstrated to elevate anti-oxidant defences through increasing Nrf2 expression, suggesting an anti-ageing effect of this flavonoid on senescent rat hearts [34]. Similar results emerged from an in vitro study on H9c2 cells in which naringenin significantly reduced the production of beta-galactosidase, a typical marker of senescence, after doxorubicin treatment [49].…”

Section: Putative Mechanisms Of Action Responsible For the Cardiovmentioning

confidence: 67%

Nutraceutical Value of Citrus Flavanones and Their Implications in Cardiovascular Disease

2017

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Background- Cardiovascular diseases, including myocardial infarction, dyslipidaemia and coronary artery pathology, are a major cause of illness and death in Western countries. Therefore, identifying effective therapeutic approaches and their cellular signalling pathways is a challenging goal for medicine. In this regard, several epidemiological studies demonstrate a relationship between the intake of flavonoid-rich foods and the reduction of cardiovascular risk factors and mortality. In particular, flavonoids present in citrus fruits, such as oranges, bergamots, lemons and grapefruit (95% from flavanones), are emerging for their considerable nutraceutical value. Methods- In this review an examination of literature was performed while considering both epidemiological, clinical and pre-clinical evidence supporting the beneficial role of the flavanone class. We evaluated studies in which citrus fruit juices or single flavanone administration and cardiovascular risk factors were analysed; to identify these studies, an electronic search was conducted in PUBMED for papers fulfilling these criteria and written in English. Results- In addition to epidemiological evidence and clinical studies demonstrating that fruits in the Citrus genus significantly reduce the incidence of cardiovascular disease risk, pre-clinical investigations highlight cellular and subcellular targets that are responsible for these beneficial effects. There has been special attention on evaluating intracellular pathways involved in direct cardiovascular and cardiometabolic effects mediated by naringenin, hesperetin and eriodictyol or their glycosylated derivatives. Conclusions- Although some mechanisms of action remain unclear and bioavailability problems remain to be solved, the current evidence supports the use of a nutraceutical approach with citrus fruits to prevent and cure several aspects of cardiovascular disease.

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Section: Putative Mechanisms Of Action Responsible For the Cardiovmentioning

confidence: 67%

Nutraceutical Value of Citrus Flavanones and Their Implications in Cardiovascular Disease

2017

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“…Some authors have found an increase of catalase expression and/or activity in cells in the presence of flavonoids [17][18][19][20][21]. This effect probably results from flavonoidinduced activation of redox-sensitive Nrf2 signaling pathway, which regulates expression of phase II detoxifying and antioxidant enzymes [21]. Others have observed no effect or even a decrease of catalase expression and activity in cells after flavonoid treatment/digestion [22,23].…”

Section: Introductionmentioning

confidence: 95%

“…The influence of flavonoids on catalase activity has been reported in some papers, the results are, however, contradictory. Some authors have found an increase of catalase expression and/or activity in cells in the presence of flavonoids [17][18][19][20][21]. This effect probably results from flavonoidinduced activation of redox-sensitive Nrf2 signaling pathway, which regulates expression of phase II detoxifying and antioxidant enzymes [21].…”

Section: Introductionmentioning

confidence: 98%

Catalase is inhibited by flavonoids

Krych

Gębicka

2013

International Journal of Biological Macromolecules

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“…This hypothesis has been verified by culturing mouse lenses with and without sulforaphane and then measuring the activity of TrxR, a very prominent member of the NADPH-disulfide oxido-reductase class of enzymes, including glutathione reductase. However, we first selected TrxR for this study in view of its activity with a wider spectrum of substrates, such as ubiquinone, lipoic acid and its amide, lipid hydro peroxide, vitamin K3, p53, hydrogen peroxide, dehydroascorbate, ascorbyl radicals, and peroxides, in addition to the protein disulfides 43–49. On the other hand, glutathione reductase is active only with glutathione disulfide (GSSG), without any effect on dehydroascorbate, peroxides, and other substrates referred to above.…”

Section: Discussionmentioning

confidence: 99%

Sulforaphane-induced transcription of thioredoxin reductase in lens: possible significance against cataract formation

Varma¹,

Chandrasekaran²,

Ковтун³

2013

OPTH

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PurposeSulforaphane is a phytochemically derived organic isothiocyanate 1-isothiocyanato-4-methylsulfinyl-butane present naturally in crucifers, including broccoli and cauliflower. Biochemically, it has been reported to induce the transcription of several antioxidant enzymes. Since such enzymes have been implicated in preventing cataract formation triggered by the intraocular generation of oxy-radical species, the purpose of this investigation was to examine whether it could induce the formation of antioxidant enzymes in the eye lens. Thioredoxin reductase (TrxR) was used as the target of such induction.MethodsMice lenses were cultured for an overnight period of 17 hours in medium 199 fortified with 10% fetal calf serum. Incubation was conducted in the absence and presence of sulforaphane (5 μM). Subsequently, the lenses were homogenized in phosphate-buffered saline (PBS), followed by centrifugation. TrxR activity was determined in the supernatant by measuring the nicotinamide adenine dinucleotide phosphate (reduced) (NADPH)-dependent reduction of 5,5′-dithiobis-2-nitrobenzoic acid (DTNB). Non-specific reduction of DTNB was corrected for by conducting parallel determinations in the presence of aurothiomalate. The reduction of DTNB was followed spectrophotometrically at 410 nm.ResultsThe activity of TrxR in the lenses incubated with sulforaphane was found to be elevated to 18 times of that observed in lenses incubated without sulforaphane. It was also noticeably higher in the lenses incubated without sulforaphane than in the un-incubated fresh lenses. However, this increase was much lower than that observed for lenses incubated with sulforaphane.ConclusionSulforaphane has been found to enhance TrxR activity in the mouse lens in culture. In view of the protective effect of the antioxidant enzymes and certain nutrients against cataract formation, the findings suggest that it would, by virtue of its ability to enhance the activity of such enzymes, prevent the tissue against oxidative stress that leads to cataract formation. Additional studies with the activities of other antioxidant enzymes such as quinone oxidoreductase and the levels of Nrf2 are in progress.

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Hesperidin-mediated expression of Nrf2 and upregulation of antioxidant status in senescent rat heart

Abstract: Hesperidin could be useful in protecting cardiomyocytes against age-related increase in oxidative stress mediated by Nrf2 upregulation.

Cited by 103 publications

References 58 publications

Nutraceutical Value of Citrus Flavanones and Their Implications in Cardiovascular Disease

Nutraceutical Value of Citrus Flavanones and Their Implications in Cardiovascular Disease

Catalase is inhibited by flavonoids

Sulforaphane-induced transcription of thioredoxin reductase in lens: possible significance against cataract formation

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