Herstellung von polyzyklischen Diterpenen

Identification of bacteria by poly-aromatic hydrocarbon biosensors

“…6 pathogenic bacterial species Escherichia coli CN13 (a nalidixic acid-resistant strain) (EC), (ATCC 700609), Bacillus subtilis (non-sporulating strain) (BS) (Difco, USA), Deinococcus radiodurans (DR) (ATCC 13939), Enterobacter cancerogenus (ENC) (isolated from drinking water and identified by Biolog System, USA), Citrobacter freundii (CF) (isolated from drinking water and identified by Biolog System, USA) were plated on nutrient agar dishes and incubated at 37 °C for 24 h. After incubation, the bacteria colonies were removed by a spatula and suspended into a small 1 mL saline solution (0.9% NaCl). Prior to fluorescence measurements, normalization of cells quantities was done by dilution of the suspension with saline followed by spectrophotometric measurements (UV-Visible spectrometer HP 8453) to obtain OD600nm = 0.05 according to Janke et al [60] Incubation of PAHs with bacteria 9-Antracene carboxylic acid (9ACA), Pyrene, Perylene, Pentacene, and Chrysene (Merck) were first dissolved in Acetone to form a concentration of 5 mM. 1 mL of each solution was diluted by 9 mL of saline solution (0.9% NaCl).…”