Herstellung von Chlorella-Farbmutanten mit Hilfe von radioaktiven Isotopen

Schwarze, P.; Frandsen, N. O.

doi:10.1007/bf00590757

Cited by 28 publications

(5 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 agar (2%) slant, Sabouraud glucose broth (SG) (glucose and enzymatic digest of casein) (DIFCO, Detroit, USA), ATCC 351 medium (Hutner's medium), ATCC 1900 medium (soil extract salts medium) and/or IAM A-7 medium (sodium acetate, beef extract, acid hydrolysate of casein and yeast extract), at 25°C in the dark. A yellow mutant (IAM C-425) and a white mutant (IAM C-539) of Chlorella vulgaris (Schwarze and Frandsen, 1960) were heterotrophically grown in IAM A-5 medium containing glucose and proteose peptone and nutrient agar (NA) (Nissui Pharmaceutical Co., Tokyo, Japan) at 20°C in the dark. Auxenochlorella protothecoides (ATCC 30407) was cultivated at 25°C in ATCC 847 medium and IAM A-4 medium in the continuous light at 3,000-5,000 lux and No.…”

mentioning

confidence: 99%

Distribution of norspermidine as a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium

Hamana

Aizaki

Arai

et al. 2004

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

mentioning

confidence: 99%

Distribution of norspermidine as a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium

Hamana

Aizaki

Arai

et al. 2004

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

“…Other genetic agents, and their mutagenic properties, began to be studied, namely chemical mutagens [ 73 ]. Reports of one of the first microalgal mutants refer back to 1960, when Schwarze and Frandsen obtained a colorless Chlorella mutant through exposure to radioactive isotopes [ 74 , 75 ]. EMS and nitrosoguanidine (MNNG) also began to be studied as mutagens applied to algae [ 76 , 77 ].…”

Section: Strategies For Microalgal Strain Improvementmentioning

confidence: 99%

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Trovão

Schüler

Machado³

et al. 2022

Marine Drugs

View full text Add to dashboard Cite

Microalgae have become a promising novel and sustainable feedstock for meeting the rising demand for food and feed. However, microalgae-based products are currently hindered by high production costs. One major reason for this is that commonly cultivated wildtype strains do not possess the robustness and productivity required for successful industrial production. Several strain improvement technologies have been developed towards creating more stress tolerant and productive strains. While classical methods of forward genetics have been extensively used to determine gene function of randomly generated mutants, reverse genetics has been explored to generate specific mutations and target phenotypes. Site-directed mutagenesis can be accomplished by employing different gene editing tools, which enable the generation of tailor-made genotypes. Nevertheless, strategies promoting the selection of randomly generated mutants avoid the introduction of foreign genetic material. In this paper, we review different microalgal strain improvement approaches and their applications, with a primary focus on random mutagenesis. Current challenges hampering strain improvement, selection, and commercialization will be discussed. The combination of these approaches with high-throughput technologies, such as fluorescence-activated cell sorting, as tools to select the most promising mutants, will also be discussed.

show abstract

“…A mutant of Chlorella kessleri, which was isolated by Schwarze and Frandsen (1960), can accumulate neither Chi nor carotenoid and, hence, becomes colorless in the light. Schmid and Schwarze (1969) showed that white cells of the mutant contained, phytoene, a precursor of carotenoid and a small amount of protoporphyrin-like pigments, suggesting defects in the biosynthetic pathways of both Chi and carotenoids.…”

mentioning

confidence: 99%

Presence of the Photoactive Reaction-Center Chlorophyll of PSI (P700) in Dark-Grown Cells of a Chlorophyll-Deficient Mutant of Chlorella kessleri

Ikegam

Kamiya

1998

Plant and Cell Physiology

View full text Add to dashboard Cite

Compositions of pigments and polypeptides of pale green membranes that had been isolated from dark-grown cells of a chlorophyll-deficient mutant of Chlorella kessleri were investigated. They contained Chi a in a level corresponding to about 1% of that present in the thylakoid membranes isolated from autotrophically grown wild-type cells and a trace amount of chlorophyllide a, but neither Chi b nor carotenoids. The polypeptide profile of the mutant membranes was similar to that of membranes isolated from wild-type cells that were grown in the dark. Neither the chlorophyll-binding subunits of PSI nor the apoproteins of LHCP were detected by SDS-PAGE and immunoblot analysis. However, the light-minus-dark difference spectrum of the mutant membranes revealed the presence of the reaction-center chlorophyll of PSI (P700) at a molar ratio of 190 chlorophyll (Chi a plus Chlide a) per P700. P700 was more stable than Chi a and Chlide a in the light so that prolonged illumination led to a decline in the Chl/P700 ratio to 24. The initial rate of P700 photooxidation in the mutant membranes was comparable to that in CP1 isolated from the dark-grown wild-type cells. Under illumination with strong light, the initial rate was decreased in parallel to the decrease in ChI/P700 ratio. The results suggest that most of Chi present in the mutant membranes can transfer excitation energy to P700.

show abstract

Herstellung von Chlorella-Farbmutanten mit Hilfe von radioaktiven Isotopen

Cited by 28 publications

References 2 publications

Distribution of norspermidine as a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium

Distribution of norspermidine as a cellular polyamine within micro green algae including non-photosynthetic achlorophyllous Polytoma, Polytomella, Prototheca and Helicosporidium

Random Mutagenesis as a Promising Tool for Microalgal Strain Improvement towards Industrial Production

Presence of the Photoactive Reaction-Center Chlorophyll of PSI (P700) in Dark-Grown Cells of a Chlorophyll-Deficient Mutant of Chlorella kessleri

Contact Info

Product

Resources

About