Herpes simplex virus type‐2 in Egyptian patients with bladder cancer or cystitis

Abstract

The present study was designed to investigate the prevalence of herpes simplex virus type-2 (HSV-2) in Egyptian patients with bladder cancer or cystitis and to evaluate the performance of different diagnostic HSV-2 assays. The study included 50 patients: 27 with bladder cancer (group I), 23 with cystitis (group II) and 20 subjects as controls (group III). HSV-2 DNA was detected using polymerase chain reaction (PCR) on bladder tissue and buffy coat cells (BCC). Electron microscopic studies (EMS) on BCC and ELISAs for IgM, IgG and specific glycoprotein G-2 (gG-2) IgG were performed. HSV-2 DNA was detected by PCR on bladder tissue biopsies in 29.6% and 21.7% of group I and II respectively and it was also detected by PCR on BCC in 22.2% and 21.7% of group I and II respectively. EMS revealed HSV like particles in 16.6% of cases. IgG, specific gG-2 IgG and IgM were detected in 30%, 16% and 6% of cases respectively. The different assays were evaluated in relation to PCR on bladder tissue biopsies. The gG-2-based ELISA and EMS on BCC were found to be highly specific (97.3% and 100% respectively), with similar low sensitivity of approximately 54%. PCR on BCC was the most sensitive assay. The association of HSV-2 with bladder cancer is suggested especially in schistosomal patients.

Entwicklung eines plattenepithelial-differenzierten Harnblasenkarzinoms bei unbehandelten Condylomen der Harnblase

“…Humane Papillom-Viren (HPV) zählen zu den am häufigsten sexuell übertragenen Erregern und sind für das unterschiedliche karzinogene Potenzial der einzelnen genetischen Subtypen (low risk-Genotypen 6 & 11, high risk-Genotypen 16 & 18 uvm.) wohl bekannt 3 4 6 . So ist HPV nachweislich mitursächlich für die Entstehung von Karzinomen an Zervix, Vulva, Oropharynx, Penis und Ösophagus 2 5 .…”

“…Der intravesikale Nachweis von HPV-Subtypen 16 & 18 konnte in Patienten mit chronischen oder rezidivierenden Zystitiden erfolgen 2 6 .…”

Etiological factors of voiding disorders in females

“…Однако вирусные агенты также вызывают развитие рецидивирующих ИНМП. По данным различных исследований, значительная часть расстройств мочеиспускания связана с вирусами, в частности с вирусами простого герпеса (ВПГ) 1 и 2-го типов, вирусом Эпштейна-Барр (ЭБВ), цитомегаловирусом и вирусом папилломы человека (ВПЧ) [7][8][9]. В зарубежной литературе имеются данные о взаимосвязи различных расстройств мочеиспускания с вирусом Varicella Zoster [10,11].…”