Herpes Simplex Keratitis in South India Clinico-Virological Correlation

Pramod, N P; Rajendran, P.; Kannan, Kalaivani; Thyagarajan, Sadras Panchatcharam

doi:10.1016/s0021-5155(99)00026-x

Cited by 21 publications

(13 citation statements)

References 12 publications

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“…There was only one sample in the control group that was considered positive by IFA but there were six cases that were negative in the test group (table 1). In our hands IFA showed better sensitivity than an earlier reported study 10 ; however, we are wary of diagnosis based on a single test and use PCR to confirm or strengthen the diagnosis. False positivity is known to be a common problem with PCR based tests 17 and during routine use, one is likely to face the problem of amplicon contamination.…”

Section: Discussioncontrasting

confidence: 62%

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Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings

Syed

2004

British Journal of Ophthalmology

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Aims: To evaluate three tests used routinely for the diagnosis of herpes simplex virus (HSV) keratitis. Methods: Corneal scrapings from 28 patients with clinically typical dendritic corneal ulcer suggestive of HSV keratitis, and 30 patients with clinically non-viral corneal ulcers, were tested by (i) Giemsa stain for multinucleated giant cells, (ii) immunofluorescence assay (IFA) for HSV-1 antigen, and (iii) polymerase chain reaction (PCR) for HSV-1 DNA, by investigators masked to clinical diagnosis. The control subjects were also investigated by smears and cultures for bacteria, fungus, and Acanthamoeba. Results: The specificity and positive predictive values of all three tests for the diagnosis of HSV keratitis were between 95-100%. The sensitivity of IFA and PCR was 78.6% and 81.2%, respectively, and the difference was not significant; however, their sensitivity and negative predictive value were significantly higher than Giemsa stain. Conclusions: While a combination of IFA and PCR constitute the choice of tests in clinically suspected cases of HSV keratitis, multinucleated giant cells in Giemsa stain can preempt testing by IFA and PCR in otherwise atypical cases of HSV keratitis.

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Section: Discussioncontrasting

confidence: 62%

“…10 The study included 234 out of 300 patients having clinical features suggestive of viral aetiology. Despite using procedures of viral culture and IFA the authors achieved an overall confirmed laboratory diagnosis in only 40% of the clinically suspected patients.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings

Syed

2004

British Journal of Ophthalmology

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“…Tear samples were used for detecting secretory IgA and/or HSV DNA. We have previously [6, 7, 8]reported the details of the prevalence study based on HSV isolation, antigen detection and tear secretory IgA ELISA. Polymerase chain reaction for HSV DNA detection was standardized using a primer sequence against the HSV thymidine kinase gene and the assay was carried out as per the method of Laycock et al [9].…”

Section: Methodsmentioning

confidence: 99%

Influence of Human Immunodeficiency Virus Status on the Clinical History of Herpes simplex Keratitis

et al. 2000

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Human immunodeficiency virus (HIV) infection is associated with a wide spectrum of systemic and ocular infectious diseases. Little is known about its association with herpes simplex keratitis (HSK) in this geographical region (South India). A retrospective study was undertaken to analyze this association in a cohort of 30 virologically proven recurrent HSK cases. Laboratory methods included herpes simplex virus (HSV) isolation, HSV antigen detection and tear secretory IgA or HSV DNA detection while commercial ELISA kits detected HIV infection. The rationale behind the HIV screening was to assess the role of HIV with increased HSK recurrences. Confirmed HIV seropositivity was 16.7% in recurrent HSK cases as against 3.3% in the matched first-episode HSK cases (p < 0.05, Fisher’s exact test). Our observations on the features of herpetic keratitis in HIV-proven patients, though based on a small number of cases, raise the question whether the immunological abnormalities associated with HIV/AIDS may affect the clinical course of HSK.

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“…It reaches the maximal concentration in the early stage after onset and could be demonstrated during the first 10 days. 8,9 The aims of our research were to analyze the HSVspecific tear sIgA and its correlation with specific serum antibodies and to evaluate the possible value of specific tear sIgA for prediction of stromal HSK recurrence.…”

mentioning

confidence: 99%

Tear HSV-Specific Secretory IgA as a Potential Indicator for Recurrent Stromal Herpes Simplex Keratitis

Huang

Wang

Zhang

2013

Cornea

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Herpes Simplex Keratitis in South India Clinico-Virological Correlation

Cited by 21 publications

References 12 publications

Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings

Diagnosis of herpes simplex virus-1 keratitis using Giemsa stain, immunofluorescence assay, and polymerase chain reaction assay on corneal scrapings

Influence of Human Immunodeficiency Virus Status on the Clinical History of Herpes simplex Keratitis

Tear HSV-Specific Secretory IgA as a Potential Indicator for Recurrent Stromal Herpes Simplex Keratitis

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