Heroin Use Promotes HCV Infection and Dysregulates HCV-Related Circulating microRNAs

Zhou, Yu; Lee, Sun; Wang, Xu; Zhou, Li; Li, Jieliang; Liu, Man‐Qing; Wang, Fang; Peng, Jinsong; Gui, Xien; Zhao, Huaqing; Reichenbach, Nancy L.; Zhou, Dun-Jin; Ho, Wen‐Zhe

doi:10.1007/s11481-014-9577-6

Cited by 27 publications

(17 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The miRNAs with the lowest expression, including hsa-miR-188-3p, hsa-miR-211-5p, hiv1-miR-TAR-3p, hsa-miR-4432 and hsa-miR-4498, were demonstrated to participate in immune regulation. For instance, hsa-miR-188-3p participates in the growth of T cells (29), hsa-miR-211-5p is dysregulated in the plasma after hepatitis C virus infection (30), and hiv1-miR-TAR-3p expression is altered following human immunodeficiency virus-1 infection (31). Furthermore, hsa-miR-4432 is associated with the pathogenesis of chronic lymphocytic leukemia, which also represents immune dysfunction (32).…”

Section: Discussionmentioning

confidence: 99%

Differential microRNA expression profile in the plasma of preeclampsia and normal pregnancies

2019

View full text Add to dashboard Cite

Preeclampsia is a common disease in pregnant women that can only be diagnosed from 20 weeks after fertilization. Developing early diagnosis markers is urgent and would be helpful in selecting appropriate treatment strategies. The present study aimed to identify the differential expression profiles of microRNAs in the plasma between patients with preeclampsia and normal pregnancies using microarray methods. Using quantitative polymerase chain reaction (qPCR), the differentially expressed microRNAs (miRNAs or miRs) identified from the microarray analysis were validated. A total of 3 miRNAs, including hsa-miR-1304-5p, hsa-miR-320a and hsa-miR-5002-5p, were upregulated in the plasma of patients with preeclampsia pregnancies. Examination of the functions of these miRNAs demonstrated that they were involved in cell proliferation, indicating that preeclampsia affected this pathway. In addition, 26 downregulated miRNAs were identified by microarray methods. The functions of these miRNAs included immune regulation, vascular development, cancer pathology and pathology of other disease (tuberculosis, oligozoospermia, psoriasis and Alzheimer's disease). Using qPCR, the most differentially expressed miRNAs were confirmed to be hsa-miR-1304-5p, hsa-miR-320a and hsa-miR-5002-5p, which were upregulated, as well as hsa-miR-188-3p, hsa-miR-211-5p, hiv1-miR-TAR-3p, hsa-miR-4432 and hsa-miR-4498 that were significantly downregulated in the plasma of preeclampsia patients. The present findings may be useful in the development of early diagnosis markers and treatment targets for preeclampsia.

show abstract

Section: Discussionmentioning

confidence: 99%

Differential microRNA expression profile in the plasma of preeclampsia and normal pregnancies

2019

View full text Add to dashboard Cite

show abstract

“…While the rate of increase in overdoses has slowed somewhat since 2006 (Chen et al, 2014), there is now concern that these decelerating rates of opioid-related deaths may be related to recent increases in heroin use, as individuals dependent on prescription opioids may be transitioning to heroin, which tends to be less expensive and more freely available (Cicero et al, 2014; Kanouse and Compton, 2015; Mateu-Gelabert et al, 2015). Heroin is among the most dangerous illicit drugs (Nutt et al, 2007; Gable, 2004) and use is associated with high rates of dependence, overdose, death, transmission of pathogens such as HIV and HCV, and social marginalization (Demaret et al, 2013; Hser et al, 2015; Hosztafi, 2011; Brown, 2015; Zhou et al, 2015). Heroin overdose deaths have increased since 2002, rising from 0.7 deaths per 100,000 to 2.7 deaths per 100,000 in 2013 (Jones et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Nonmedical opioid use and heroin use in a nationally representative sample of us high school seniors

Palamar

Shearston

Dawson

et al. 2016

Drug and Alcohol Dependence

View full text Add to dashboard Cite

Background Nonmedical use of opioids has become increasingly problematic in recent years with increases in overdoses, treatment admissions, and deaths. Use also appears to be contributing to heroin initiation, which has increased in recent years. Further research is needed to examine which adolescents are at highest risk for nonmedical use of opioids and heroin and to explore potential links between nonmedical opioid use and heroin use. Methods Data were analyzed from a nationally representative sample of American high school seniors in the Monitoring the Future study (2009–2013, Weighted N = 67,822). We examined associations between frequency and recency of nonmedical use of opioids and heroin. Sociodemographic correlates of use of each drug were also examined. Results 12.4% of students reported lifetime nonmedical opioid use and 1.2% reported lifetime heroin use. As frequency of lifetime nonmedical opioid use increased, so too did the odds for reporting heroin use, with over three-quarters (77.3%) of heroin users reporting lifetime nonmedical opioid use. Recent (30-day) nonmedical opioid use was a robust risk factor for heroin use and almost a quarter (23.2%) of students who reported using opioids ≥40 times reported lifetime heroin use. Black and Hispanic students were less likely to report nonmedical opioid or heroin use than white students, but they were more likely to report heroin use in absence of nonmedical opioid use. Discussion Recent and frequent nonmedical opioid use are risk factors for heroin use among adolescents. Prevention needs to be targeted to those at highest risk.

show abstract

“…Opiate use is associated with the worst health outcomes in HIV-infected individuals, as they adversely impact the human immune system. Clinical and epidemiological evidence from pre-AIDS studies indicate that opiates (particular heroin) play a cofactor role in the pathogenesis of HIV infection ( Donahoe et al, 1993 ; Ronald et al, 1994 ; Specter, 1994 ; Meijerink et al, 2015 ; Zhou et al, 2015 ). In vitro studies from different laboratories also provide direct evidence that opiates (morphine) facilitate HIV infection of the target cells.…”

Section: Introductionmentioning

confidence: 99%

Heroin inhibits HIV-restriction miRNAs and enhances HIV infection of macrophages

Wang

et al. 2015

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

Although opioids have been extensively studied for their impact on the immune system, limited information is available about the specific actions of opioids on intracellular antiviral innate immunity against HIV infection. Thus, we investigated whether heroin, one of the most abused drugs, inhibits the expression of intracellular HIV restriction microRNA (miRNA) and facilitates HIV replication in macrophages. Heroin treatment of macrophages enhanced HIV replication, which was associated with the downregulation of several HIV restriction miRNAs. These heroin-mediated actions on the miRNAs and HIV could be antagonized by naltrexone, an opioid receptor antagonist. Furthermore, the in vitro negative impact of heroin on HIV-associated miRNAs was confirmed by the in vivo observation that heroin addicts had significantly lower levels of macrophage-derived HIV restriction miRNAs than those in the control subjects. These in vitro and in vivo findings indicate that heroin use compromises intracellular anti-HIV innate immunity, providing a favorable microenvironment for HIV survival in the target cells.

show abstract

Heroin Use Promotes HCV Infection and Dysregulates HCV-Related Circulating microRNAs

Cited by 27 publications

References 57 publications

Differential microRNA expression profile in the plasma of preeclampsia and normal pregnancies

Differential microRNA expression profile in the plasma of preeclampsia and normal pregnancies

Nonmedical opioid use and heroin use in a nationally representative sample of us high school seniors

Heroin inhibits HIV-restriction miRNAs and enhances HIV infection of macrophages

Contact Info

Product

Resources

About