Heritable Variation in Pea for Resistance Against a Root Rot Complex and Its Characterization by Amplicon Sequencing

Wille, Lukas; Messmer, Monika; Bodenhausen, Natacha; Studer, Bruno; Hohmann, Pierre

doi:10.3389/fpls.2020.542153

Cited by 14 publications

(22 citation statements)

References 79 publications

(89 reference statements)

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“…Similarly, it has been shown that arbuscular mycorrhizal fungi (AMF) increase the resistance of pea against Aphanomyces root rot (Thygesen et al, 2004). Recently, both microbial taxa were found within a diverse fungal community that included several known pea pathogens (Wille et al, 2020). In line with that, the study of Xu et al (2012a) has previously shown that the health status of pea is mainly related to the fungal community present in diseased roots but barely reflected by the fungal community in the soil.…”

Section: Introductionmentioning

confidence: 76%

“…The experiment involved eight peas (P. sativum L.) genotypes that were selected based on a previous study on root rot resistance. These genotypes showed contrasting levels of resistance to a PRRC present in naturally infested field soil (Kirchlindach) (Wille et al, 2020). The present selection includes four varieties and four genebank accessions from the USDA-ARS GRIN Pea Core Collection (Supplementary Table 1).…”

Section: Plant Growth and Phenotypingmentioning

confidence: 99%

“…The plants were removed from the pots 29 days after sowing, and roots were washed under running tap water. A root rot index [RRI; 1 = healthy; 6 = complete root rot, plant dead (Wille et al, 2020)] was attributed to individual plants. Roots were separated from shoots with clean scissors, and kept on ice before storage at -20 • C. Shoots were dried at 105 • C until constant weight before recording dry weight.…”

Section: Plant Growth and Phenotypingmentioning

confidence: 99%

“…Previously published qPCR assays were used to quantify ten microbial taxa in the roots of plants grown in the nonsterile treatment (Supplementary Table 3). Microbial taxa were selected based on information from previous studies, including a characterisation of the fungal community of diseased pea roots (Wille et al, 2020). As a control, roots of pea genotypes C1 and C2 grown in the sterilised soil were also analysed.…”

Section: Quantification Of Microbial Taxa In Diseased Pea Rootsmentioning

confidence: 99%

“…In each soil, F. solani and F. oxysporum consistently showed high 5] of the 10 microbial taxa are given, with pathogens extending above of the 0-scale bar, beneficial taxa below (AMF quantifications were square-root transformed for this presentation). Pea genotypes are ordered based on relative shoot dry weight (high to low) in the initial resistance screening (Wille et al, 2020). connectivity (no.…”

Section: Quantification and Composition Of Key Microbial Taxamentioning

confidence: 99%

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Untangling the Pea Root Rot Complex Reveals Microbial Markers for Plant Health

et al. 2021

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Plant health is recognised as a key element to ensure global food security. While plant breeding has substantially improved crop resistance against individual pathogens, it showed limited success for diseases caused by the interaction of multiple pathogens such as root rot in pea (Pisum sativum L.). To untangle the causal agents of the pea root rot complex and determine the role of the plant genotype in shaping its own detrimental or beneficial microbiome, fungal and oomycete root rot pathogens, as well as previously identified beneficials, i.e., arbuscular mycorrhizal fungi (AMF) and Clonostachys rosea, were qPCR quantified in diseased roots of eight differently resistant pea genotypes grown in four agricultural soils under controlled conditions. We found that soil and pea genotype significantly determined the microbial compositions in diseased pea roots. Despite significant genotype x soil interactions and distinct soil-dependent pathogen complexes, our data revealed key microbial taxa that were associated with plant fitness. Our study indicates the potential of fungal and oomycete markers for plant health and serves as a precedent for other complex plant pathosystems. Such microbial markers can be used to complement plant phenotype- and genotype-based selection strategies to improve disease resistance in one of the world’s most important pulse crops of the world.

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Section: Introductionmentioning

confidence: 76%

Section: Plant Growth and Phenotypingmentioning

confidence: 99%

Section: Plant Growth and Phenotypingmentioning

confidence: 99%

Section: Quantification Of Microbial Taxa In Diseased Pea Rootsmentioning

confidence: 99%

Section: Quantification and Composition Of Key Microbial Taxamentioning

confidence: 99%

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Untangling the Pea Root Rot Complex Reveals Microbial Markers for Plant Health

et al. 2021

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Evaluation of Pea Accessions Differing in Flower and Seed Coat Pigmentation for Resistance to Fusarium avenaceum Root Rot

Awodele,

Gali,

Sivachandra Kumar

et al. 2024

Legume Science

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Pea production across the world is significantly limited by root rot disease, which is caused by many fungal and oomycetes pathogens. In Canada, Fusarium avenaceum is the most devastating pathogen of the Fusarium root rot complex of pea. Host genetic resistance is the most effective control method for this disease. Evaluation of global pea accessions and Canadian varieties for F. avenaceum root rot resistance has not been reported to date. This study evaluated 20 pea accessions of different market classes with pigmented or nonpigmented seed coats and flowers for F. avenaceum resistance under controlled conditions. The pea accessions CDC Acer, CDC Vienna, PBA OURA, Morgan, CDC Blazer, CDC Dakota, and PI 280609, which have pigmented flowers and seed coats, were identified as resistant or partially resistant to F. avenaceum. This was based on their root rot severity scores and ability to tolerate F. avenaceum infection without significant (p > 0.05) reductions in plant height, shoot dry weight, and root dry weight. Among the varieties with nonpigmented flowers and seed coats, only Cameor showed partial resistance to F. avenaceum when challenged with reduced conidial concentration. Root dry weight (R = −0.86), plant height (R = −0.82), and shoot dry weight (R = −0.78) had a strong negative correlation (p < 0.001) with disease severity, suggesting that F. avenaceum root rot can negatively impact the growth and development of pea seedlings. F. avenaceum resistance identified in this study can be utilized to study the molecular basis of the resistance and develop disease‐resistant varieties. While our findings suggest a relationship between pigmentation and F. avenaceum resistance, future research with a larger, more diverse panel is warranted to validate these initial results.

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