Heritable nanosilver resistance in priority pathogen: a unique genetic adaptation and comparison with ionic silver and antibiotics

Valentin, Elizabeth; Bottomley, Amy L.; Chilambi, Gayatri Shankar; Harry, Elizabeth J.; Amal, Rose; Sotiriou, Georgios A.; Rice, Scott A.; Gunawan, Cindy

doi:10.1039/c9nr08424j

Cited by 33 publications

(42 citation statements)

References 59 publications

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“…Over several years, a growing number of studies have been published describing the phenomenon of resistance in bacterial species in response to different forms of silver agents, including NAg. Silver resistance has been reported in A. baumannii and many other important pathogenic bacteria (Gupta et al, 1999;Gunawan et al, 2013;Muller and Merrett, 2014;Panáček et al, 2018;Hosny et al, 2019;Valentin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

et al. 2021

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The misuse of antibiotics combined with a lack of newly developed ones is the main contributors to the current antibiotic resistance crisis. There is a dire need for new and alternative antibacterial options and nanotechnology could be a solution. Metal-based nanoparticles, particularly silver nanoparticles (NAg), have garnered widespread popularity due to their unique physicochemical properties and broad-spectrum antibacterial activity. Consequently, NAg has seen extensive incorporation in many types of products across the healthcare and consumer market. Despite clear evidence of the strong antibacterial efficacy of NAg, studies have raised concerns over the development of silver-resistant bacteria. Resistance to cationic silver (Ag+) has been recognised for many years, but it has recently been found that bacterial resistance to NAg is also possible. It is also understood that exposure of bacteria to toxic heavy metals like silver can induce the emergence of antibiotic resistance through the process of co-selection. Acinetobacter baumannii is a Gram-negative coccobacillus and opportunistic nosocomial bacterial pathogen. It was recently listed as the “number one” critical level priority pathogen because of the significant rise of antibiotic resistance in this species. NAg has proven bactericidal activity towards A. baumannii, even against strains that display multi-drug resistance. However, despite ample evidence of heavy metal (including silver; Ag+) resistance in this bacterium, combined with reports of heavy metal-driven co-selection of antibiotic resistance, little research has been dedicated to assessing the potential for NAg resistance development in A. baumannii. This is worrisome, as the increasingly indiscriminate use of NAg could promote the development of silver resistance in this species, like what has occurred with antibiotics.

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Section: Introductionmentioning

confidence: 99%

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

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“…The upregulation of efflux pumps that can extrude metal ions and antibiotics represents the most conspicuous example of this [71]. The increased abundance of efflux pump genes in soil and water environments under NP exposure has been proven by several studies [14,15,35,56,61,62]. As stated above, some efflux pumps of the RND family are known to export antibiotics in P. aeruginosa [83].…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 92%

“…Indeed, overexpression of genes encoding ROS scavenging systems have been demonstrated in E. coli, B. subtilis, and P. aeruginosa PAO1 exposed to Ag-NPs, Al2O3-NPs, and Cd-QDs, respectively [56,57,64], and have been correlated with a better capacity to tolerate prolonged exposure to Ag-NPs [52]. Furthermore, recent work carried out by Valentin and collaborators [14] reported that Ag-NP resistance evolved in S. aureus was associated with mutations in genes involved in nucleotide synthesis and oxidative stress defenses.…”

Section: Bacterial Resistance Mechanisms Towards Metallic Nanoparticlesmentioning

confidence: 97%

“…Furthermore, the non-specific mode of action of NPs toward multiple cellular components suggested that development of resistance is less likely to occur, as bacteria would have to acquire multiple mutations [41,43,45]. In line with this hypothesis, Valentin and coworkers recently showed that Staphylococcus aureus more readily developed resistance to ciprofloxacin than to 2-nm Ag-NPs [14]. However, over the last years, a growing body of research has evidenced that bacteria can indeed evolve defense strategies to cope with metallic NPs ( Figure 1B).…”

Section: Bacterial Resistance Mechanisms Towards Metallic Nanoparticlesmentioning

confidence: 99%

“…Because NPs display multiple antibacterial mechanisms, it has been thought that bacteria are unlikely to develop resistance against these nanomaterials. However, recent studies have shown that bacteria can in fact tolerate increasing concentrations of copper and silver NPs [12][13][14][15]. Hence, concerns are rising about whether bacteria may develop resistance towards the widely commercialized NP-based antimicrobial materials.…”

Section: Introductionmentioning

confidence: 99%

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Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

et al. 2021

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The rapid spread of antibiotic resistances among bacteria demands novel strategies for infection control, and metallic nanoparticles appear as promising tools because of their unique size and tunable properties that allow their antibacterial effects to be maximized. Furthermore, their diverse mechanisms of action towards multiple cell components have suggested that bacteria could not easily develop resistance against nanoparticles. However, research published over the last decade has proven that bacteria can indeed evolve stable resistance mechanisms upon continuous exposure to metallic nanoparticles. In this review, we summarize the currently known individual and collective strategies employed by bacteria to cope with metallic nanoparticles. Importantly, we also discuss the adverse side effects that bacterial exposure to nanoparticles may have on antibiotic resistance dissemination and that might constitute a challenge for the implementation of nanoparticles as antibacterial agents. Overall, studies discussed in this review point out that careful management of these very promising antimicrobials is necessary to preserve their efficacy for infection control.

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Nanostructured Ag‐Bioglass Implant Coatings with Antibacterial and Osteogenic Activity

Geissel

Platania

DeBerardinis

et al. 2022

Adv Materials Inter

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Bone implant failure due to aseptic loosening and biofilm infections is an increasing healthcare problem. Implants may be coated with nanoparticles to avoid bacterial colonization and promote osseointegration. However, these nanocoatings often require long, expensive, and complex manufacturing routes with limited clinical translation potential. Here, a multifunctional nanoparticle coating consisting of silver (Ag) and bioglass (BG) is investigated to overcome current limitations by providing synchronously antibacterial and osteogenic effect. Flame spray pyrolysis (FSP) is exploited as a scalable and reproducible process to synthesize large quantities of nanoparticles and deposit them on titanium (Ti) substrates. The deposited nanocoatings show a homogeneous morphology and biomineralize after soaking in simulated body fluid (SBF), while their adhesion on Ti substrates is promoted by in situ flame annealing. The Ag+ ion release from Ag containing BG samples inhibits Staphylococcus aureus biofilm formation up to 3 log units, while the osteogenic responses of pre‐osteoblastic cells directly grown on AgBG samples show similar levels of alkaline phosphatase activity, calcium and collagen production when compared to pure Ti. The inexpensively synthesized multifunctional AgBG nanostructured implant coatings exert a high bioactivity and antibacterial response while maintaining high biocompatibility. The insights of this study can direct the development of multifunctional implant coatings.

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Heritable nanosilver resistance in priority pathogen: a unique genetic adaptation and comparison with ionic silver and antibiotics

Abstract: A priority pathogen is capable to evolve stable resistance characteristics that still manifest after discontinuation of the nanosilver exposure.

Cited by 33 publications

References 59 publications

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

Emerging Concern for Silver Nanoparticle Resistance in Acinetobacter baumannii and Other Bacteria

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

Nanostructured Ag‐Bioglass Implant Coatings with Antibacterial and Osteogenic Activity

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