Hericium caput-medusae (Bull.:Fr.) Pers. polysaccharide enhance innate immune response, immune-related genes expression and disease resistance against Aeromonas hydrophila in grass carp (Ctenopharyngodon idella)

Gou, Changlong; Wang, Jiazhen; Wang, Yuqiong; Dong, Wenlong; Shan, Xiaofeng; Lou, Yujie; Gao, Yunhang

doi:10.1016/j.fsi.2017.11.027

Cited by 64 publications

(19 citation statements)

References 46 publications

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“…The immunity of fish largely depends on humoral components, such as lysozyme, C3 and C4. Lysozyme is a significant component of the innate immune system extensively distributed in body fluids, which is a reliable indicator reflecting a fish's health status (Gobi, Vaseeharan, & Rekha, ; Gou et al, ). It can dissolve bacteria, activate the complement system and serve a defensive function of destroying and removing foreign materials (Bergljót, ).…”

Section: Discussionmentioning

confidence: 99%

“…The serum lysozyme activity (LZM), complement 3 (C3) and complement 4 (C4) levels were determined using the turbidimetric and immune turbidimetric methods (Nanjing Jiancheng Bioengineering Institute). The LZM activity was determined by adding serum to a suspension of Micrococcus lysodeikticus , and the absorbance at 520 nm was determined at 0.5 and 4.5 min (Gou et al, ). C3 and C4 activity assays were performed according to the method of Vibertigc (), including an increase in turbidity following an immune response and an increase in antibody.…”

Section: Methodsmentioning

confidence: 99%

“…The PCR reaction system included 10 μl of SYBR PCR Mix (2×), 1 μl of PCR forward primer (10 μM), 1 μl of PCR reverse primer (10 μM), 1 μl of cDNA and 7 μl of DEPC‐treated water. The reaction conditions were as follows: 95℃ for 30 s, followed by 40 cycles of 95℃ for 5 s, annealing for 30 s and 72℃ for 30 s. According to Gou's method (), the relative gene expression results were determined taking β‐actin as an internal referenced and calculated using the

2^{- normalΔ normalΔ C_{t}}

method.…”

Section: Methodsmentioning

confidence: 99%

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Effects of dietary astaxanthin on growth, blood biochemistry, antioxidant, immune and inflammatory response in lipopolysaccharide‐challengedChanna argus

Zhu

Liu

et al. 2020

Aquac Res

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The present study investigated the effects of dietary astaxanthin on the growth, blood biochemical, antioxidant, immune and inflammatory response in lipopolysaccharide‐challenged Channa argus. A total of 0, 50, 100 and 200 mg/kg of astaxanthin were added to the basal die for 56 days. After the feeding experiment, each group was subjected to a lipopolysaccharide challenge (except for the Control group). The results showed that adding astaxanthin to the diet can significantly increase the weight gain and specific growth rate and decrease the feed conversion ratio of C. argus; the highest weight gain, specific growth rate and minimum feed conversion ratio occurred in the 100 mg/kg group. Furthermore, dietary astaxanthin supplementation can alleviate the negative effects of lipopolysaccharides by increasing the levels of alkaline phosphatase, lysozyme, complement 3, complement 4, total serum protein, albumin, globulin, superoxide dismutase, catalase and glutathione peroxidase and decrease the serum cortisol, aspartate aminotransferase, alanine aminotransferase, glutathione peroxidase, and malondialdehyde. Dietary astaxanthin supplementation also can decrease the relative expression of inflammatory genes (nuclear factor κB, interleukin‐1, interleukin‐8 and tumour necrosis factor‐α) in the liver, spleen, kidney and intestine. To summarise, dietary astaxanthin addition can improve the growth performance and attenuate the negative effects of lipopolysaccharide challenge in C. argus. The optimal amount of astaxanthin is 100 mg/kg.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

2^{- normalΔ normalΔ C_{t}}

method.…”

Section: Methodsmentioning

confidence: 99%

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Effects of dietary astaxanthin on growth, blood biochemistry, antioxidant, immune and inflammatory response in lipopolysaccharide‐challengedChanna argus

Zhu

Liu

et al. 2020

Aquac Res

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“…For instance, the addition of Ganoderma lucidum polysaccharide to the basic diet significantly improved the survival and growth rates and the digestive enzyme activities of Macrobrachium rosenbergii (12). Both Ficus carica polysaccharide and Hericium caput-medusae polysaccharide stimulated the immune response in grass carp, and upregulated the immune-related genes, thereby enhancing the host's disease resistance ability (3,13). Polysaccharides from Padina gymnospora and Astragalus sp.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Oral Rehmannia glutinosa Polysaccharide Administration on Immune Responses, Antioxidant Activity and Resistance Against Aeromonas hydrophila in the Common Carp, Cyprinus carpio L

et al. 2020

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The effects of the oral administration of Rehmannia glutinosa polysaccharide (RGP-1) on the immunoregulatory properties, antioxidant activity, and resistance against Aeromonas hydrophila in Cyprinus carpio L. were investigated. The purified RGP-1 (250, 500, and 1,000 µg/mL) was co-cultured with the head kidney cells of the common carp. The proliferation and phagocytosis activities of the head kidney cells, and the concentration of nitric oxide (NO) and cytokines in the culture medium were determined. Next, 300 common carps (47.66 ± 0.43 g) were randomly divided into five groups; the two control groups (negative and positive) were administered sterile PBS and the three treatment groups were administered different concentrations of RGP-1 (250, 500, and 1,000 µg/mL) for seven days. Subsequently, the positive and treatment groups were infected with A. hydrophila, and the negative group was administered sterile PBS for 24 h. The concentration of NO, cytokines, lysozyme (LZM), and alkaline phosphatase (AKP) in serum, the total antioxidant capacity (T-AOC), the levels of malonaldehyde (MDA) and glutathione (GSH), and the total activities of superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) in the hepatopancreas of the common carp were tested. We observed that RGP-1 could significantly enhance the proliferation and phagocytosis activities (P < 0.05), besides inducing the production of NO, pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12) and anti-inflammatory cytokines (IL-10, TGF-β) (P < 0.05) in vitro. The in vivo experimental results revealed that RGP-1 significantly enhanced NO production, protein levels of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12), LZM and AKP activities, and the antioxidant content (T-AOC, SOD, CAT, GSH, GSH-Px, and MDA) compared to that observed in the negative group prior to A. hydrophila infection (P < 0.05). NO, pro-inflammatory cytokines, LZM Feng et al. RGP-1 as Immunostimulant in Commom Carp and AKP activities were significantly lower than that in the positive group after infection (P < 0.05). However, whether infected or not, the expression of anti-inflammatory cytokines (IL-10, TGF-β) increased significantly in the RGP-1-treated groups (P < 0.05). Therefore, the results suggested that RGP-1 could enhance the non-specific immunity, antioxidant activity and anti-A. hydrophila activity of the common carp, and could be used as a safe and effective feed additive in aquaculture.

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“…chicken-type (c-Lyz) and goose-type (g-Lyz) (Callewaert & Michiels, 2010;Callewaert et al, 2012;Zhang et al, 2018). The c-and g-type of lysozymes have been demonstrated to play important roles in the immune defense in many fish species, especially in protection against pathogenic bacterial infection (Liu et al, 2016;Wang et al, 2016;Di Falco et al, 2017;Gou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish

Nasrullah¹,

Nababan²,

Yanti³

et al. 2019

Jurnal Akuakultur Indonesia

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Lysozymes play an important role in the first line of defense in fish and potentially used as an immunity status biomarker and immune responses evaluation in fish, which often found in two types, i.e. chicken-type and goose-type (c- and g-type, respectively). To recent, the information related to the sequences and the expression analysis of the c- and g-type lysozyme genes in African catfish is still limited. In the present study, we report a partial cloning and mRNA expression analysis of c-type and g-type lysozymes in African catfish Clarias gariepinus. We have successfully cloned and partially identify the c-type, and g-type lysozyme genes of C. gariepinus, which consist of 594 and 560 of coding sequences, respectively. Catalytic and other conserved residues were identified by multiple sequences alignment and they showed high similarity with other teleost fish species. mRNA levels of the genes were analyzed by using qPCR method and their expressions in the spleen, liver, and head kidney were rapidly modulated after Aeromonas hydrophila injection, with different patterns were observed in each organ. These results confirmed that c- and g-type lysozymes played an important role in non-specific immunity against A. hydrophila infection. This study provided valuable information that can be used to understand the African catfish immune systems for better disease and stress management in C. gariepinus culture.Keywords: lysozymes, gene identification, gene expression, bacterial infection, African catfish ABSTRAKLisozim berperan dalam sistem pertahanan dini pada ikan dan sangat potensial digunakan sebagai marka status imunitas dalam evaluasi respons imun. Lisozim umum ditemukan dalam dua tipe pada ikan: tipe-ayam (tipe-c) dan tipe-angsa (tipe-g). Informasi terkait sekuens gen dan ekspresi gen kedua tipe lisozim pada ikan lele dumbo sangat terbatas. Pada penelitian ini, kami melaporkan kloning gen secara parsial, dan analisis ekspresi gen dari kedua tipe lisozim pada ikan lele dumbo C. gariepinus. Sekuens parsial gen lisozim tipe-c dan tipe-g yang berhasil diidentifikasi adalah sepanjang 594 dan 560 pasang basa. Situs katalitik dan residu khas memiliki tingkat kesamaan yang tinggi dengan spesies ikan yang lain. Analisis mRNA dilakukan dengan metode quantitative PCR (qPCR). Ekspresi kedua gen di jaringan ginjal depan, limpa, dan hati dengan cepat terinduksi pasca infeksi bakteri A. hydrophila dengan pola yang berbeda. Hasil ini menunjukkan bahwa lisozim tipe-c dan tipe-g memiliki peran yang sangat penting dalam sistem imun ikan lele dumbo terhadap infeksi A. hydrophila. Penelitian ini menghasilkan informasi penting yang dapat digunakan untuk mempelajari sistem imun ikan lele dumbo dan sebagai acuan dalam penanganan dan manajemen penyakit pada budidaya ikan lele dumbo.Kata kunci: lisozim, identifikasi gen, ekspresi gen, infeksi bakteri, ikan lele dumbo

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Hericium caput-medusae (Bull.:Fr.) Pers. polysaccharide enhance innate immune response, immune-related genes expression and disease resistance against Aeromonas hydrophila in grass carp (Ctenopharyngodon idella)

Cited by 64 publications

References 46 publications

Effects of dietary astaxanthin on growth, blood biochemistry, antioxidant, immune and inflammatory response in lipopolysaccharide‐challengedChanna argus

Effects of dietary astaxanthin on growth, blood biochemistry, antioxidant, immune and inflammatory response in lipopolysaccharide‐challengedChanna argus

The Effects of Oral Rehmannia glutinosa Polysaccharide Administration on Immune Responses, Antioxidant Activity and Resistance Against Aeromonas hydrophila in the Common Carp, Cyprinus carpio L

Identification and expression analysis of c-type and g-type lysozymes genes after Aeromonas hydrophila infection in African catfish

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