Heregulin-β1 promotes metastasis of breast cancer cell line SKBR3 through upregulation of Snail and induction of epithelial-mesenchymal transition

Cheng, Luyang; Zha, Zhao; Liu, Bo; Liu, Jing; Yao, Xuebiao

doi:10.1016/j.canlet.2009.02.007

Cited by 18 publications

(29 citation statements)

References 36 publications

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“…HRG has been shown to play a relevant role for the aggressiveness and metastatic capacity of breast cancer cells (27,28) and is associated with gefitinib resistance (35). Our study revealed that HRG regulates CXCR4 expression.…”

Section: Discussionmentioning

confidence: 51%

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An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

Park

Kook

Lee

et al. 2013

Journal of Biological Chemistry

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Background: CXCR4/CXCL12 axis provides directional cues for breast cancer cells to metastasize to specific organs. Results: LIP regulates the expression of CXCR4, promoting CXCR4/CXCL12-mediated breast cancer cell migration. Conclusion: LIP is a previously unrecognized transcriptional regulator of CXCR4. Significance: Our results revealed a potential link between heregulin, LIP, and CXCR4 and may have important therapeutic implications for metastatic breast cancer.

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Section: Discussionmentioning

confidence: 51%

“…Cancer Cells-HRG promotes metastatic migration of breast cancer cells (27,28), and blockage of HRG expression inhibits metastasis of breast cancer (18). Interestingly, HRG treatment increased the levels of LIP but not the other C/EBP␤ isoforms (Fig.…”

Section: Heregulin Up-regulates the Expression Of Cxcr4 In Breastmentioning

confidence: 99%

An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

Park

Kook

Lee

et al. 2013

Journal of Biological Chemistry

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“…On the other hand, aberrant expression and activation of the ErbB system (e.g., phosphorylated EGFR, ErbB2 and/ or ErbB3) and overexpressed ErbB ligands (e.g., TGF-α and amphiregulin) are linked to endocrine therapy resistance in both breast cancer cells and breast tumors (65)(66)(67). Previous studies have suggested that HRGs are sufficient for breast cancer initiation and progression by promoting metastasis and angiogenesis; even in the absence of estrogen stimulation and the independence of ErbB2 overexpression (21,22,32,(67)(68)(69). The blockage of HRG expression suppresses the aggressive phenotype by inhibiting tumor growth and metastasis (32).…”

Section: Discussionmentioning

confidence: 99%

“…Since GPR30 and HRG-β1 signaling has been reported to play a role in tumor metastasis (8,22,(30)(31)(32), we investigated whether GPR30 plays a role in the migration and invasion induced by HRG-β1. The migration of MCF-7 cells was analyzed with a Boyden chamber migration assay.…”

Section: Elevation Of Gpr30 Promotes the Migration And Invasion Inducmentioning

confidence: 99%

“…The binding of HRGs to the extracellular domain of the receptor tyrosine kinases, ErbB3 and ErbB4, leads to the formation of ErbB homo-or heterodimers, which in turn activates intracellular signaling pathways and drives cell proliferation, adhesion, differentiation and migration (17). In breast cancer, it has been reported that HRGs contribute to the acquisition of anti-hormonal drug resistance characteristic (18,19) and distant metastasis (20)(21)(22).…”

Section: Introductionmentioning

confidence: 99%

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Regulation of HRG-β1-induced proliferation, migration and invasion of MCF-7 cells by upregulation of GPR30 expression

Zhang

2012

Mol Med Report

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Abstract. The cooperation and communication between different cell signaling transduction pathways are considered critical in the development of various types of cancer as well as drug resistance. There is evidence of crosstalk between the G protein-coupled receptor 30 (GPR30), the newly discovered estrogen receptor (ER), and the ErbB family. Heregulin (HRG)-β1, the ligand for ErbB3 and ErbB4, upregulates GPR30 expression in MCF-7, T-47D and BT-474 breast cancer cell lines that express ERα. In the present study, recombinant human HRG-β1 was used to investigate the upregulation of GPR30 expression by HRGs in MCF-7 breast cancer cells which were ERα-positive. In MCF-7 cells, the ErbB2 inhibitor, AG825, the MAPK inhibitor, PD98059, and the MEK1/2 inhibitor, U0126, blocked the HRG-β1-induced GPR30 expression. 17-β-estradiol (E2) boosted the HRG-β1-induced proliferation, migration and invasion of MCF-7 cells. Similar to E2, the specific GPR30 agonist, G-1, promoted HRG-β1-induced migration and invasion, but inhibited growth. Using the specific GPR30 antagonist, G-15, or the small interfering RNA for GPR30, the functions of GPR30 after treatment with HRG-β1 were further investegated. The results from our study indicate that the interruption between GPR30 signaling and the ErbB family system may serve as a promising therapeutic strategy for breast cancer.

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Targeted Inhibition of Snail Activity in Breast Cancer Cells by Using a Co^III‐Ebox Conjugate

et al. 2015

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The transition from a non-invasive to an invasive phenotype is an essential step in tumor metastasis. The Snail family of transcription factors (TFs) is known to play a significant role in this transition. These TFs are zinc fingers that bind to the CAGGTG Ebox consensus sequence. CoIII-Ebox is a cobalt(III) complex attached to an Ebox oligonucleotide that confers specificity towards Snail TFs. CoIII-Ebox has been shown to inhibit Snail-mediated embryonic neural crest development in Xenopus laevis, but its efficacy in inhibiting Snail-induced cancer cell invasiveness has not been explored. Here, we describe the efficacy of CoIII-Ebox in inhibiting the invasive aspects of heregulin β1(HRG)-treated breast cancer cells. CoIII-Ebox was found to inhibit the capacity of Snail to repress target genes after HRG induction. Snail inhibition by CoIII-Ebox reduced the invasive propensity of cells in 2D and 3D, thereby demonstrating promise in inhibiting metastasis.

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Heregulin-β1 promotes metastasis of breast cancer cell line SKBR3 through upregulation of Snail and induction of epithelial-mesenchymal transition

Cited by 18 publications

References 36 publications

An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

Regulation of HRG-β1-induced proliferation, migration and invasion of MCF-7 cells by upregulation of GPR30 expression

Targeted Inhibition of Snail Activity in Breast Cancer Cells by Using a Co^III‐Ebox Conjugate

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Heregulin-β1 promotes metastasis of breast cancer cell line SKBR3 through upregulation of Snail and induction of epithelial-mesenchymal transition

Cited by 18 publications

References 36 publications

An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

An Isoform of C/EBPβ, LIP, Regulates Expression of the Chemokine Receptor CXCR4 and Modulates Breast Cancer Cell Migration

Regulation of HRG-β1-induced proliferation, migration and invasion of MCF-7 cells by upregulation of GPR30 expression

Targeted Inhibition of Snail Activity in Breast Cancer Cells by Using a CoIII‐Ebox Conjugate

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Targeted Inhibition of Snail Activity in Breast Cancer Cells by Using a Co^III‐Ebox Conjugate