Herbs of interest to the Brazilian Federal Government: female reproductive and developmental toxicity studies

Veríssimo, Luiz Fernando; Bacchi, André Demambre; Zaminelli, Tiago; Paula, Gustavo Henrique O. de; Moreira, Estefânia Gastaldello

doi:10.1590/s0102-695x2011005000146

Cited by 10 publications

(8 citation statements)

References 39 publications

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“…Because of this, some patients use affordable and cost effective alternative therapy for management of diabetes in the form of traditional medicines, which are both locally available and cheap 2, 4 . Several herbal remedies used in the management of diabetes have been reported to possess hypoglycemic effects [6][7][8][9][10][11] . Among these is Momordica charantia L Fam.…”

Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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Background: Momordica charantia L. is a medicinal plant commonly used in the management of diabetes mellitus. Objectives: We investigated the blood glucose lowering effect of the methanolic fruit extract of the Ugandan variety of M. charantia L. in alloxan-induced diabetic albino rats. Methods: 500g of M. charantia powder were macerated in methanol and the extract administered to two groups of alloxaninduced diabetic rats. The first group received 125mg/kg, the second 375mg/kg and a third group 7mg/ kg of metformin. A fourth group received 1ml normal saline. Fasting blood glucose (FBG) levels were measured at 0.5,1,2,3,5,8 and 12 hours and compared using one-way ANOVA. Results: There was an initial rise in FBG for 1 hour after administration of extracts followed by steep reductions. Significant reduction in FBG occurred at 2 hours for 125mg/kg of extract (-3.2%, 313±25.9 to 303±25.0mg/dL, p = 0.049), 375mg/ kg of extract (-3.9%, 356±19.7 to 342±20.3mg/dL, p = 0.001), and metformin (-2.6%, 344±21.7 to 335±21.1mg/dL, p = 0.003) when compared to normal saline. The maximum percentage reduction in FBG by both extracts occurred between 3 and 12 hours post dose. Conclusions: The methanolic fruit extract of M. charantia exhibits dose dependent hypoglycaemic activity in vivo.

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Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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“…The most common medicinal use of M. charantia is as an effective treatment of diabetes. Number of studies have been conducted to determine the safety profile of M. charantia in experimental animals; and all have found that M. charantia did not show any kind of toxicity when tested in non-pregnant animals [8, 51–56].…”

Section: Discussionmentioning

confidence: 99%

Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos

Farooq

Abutaha

Nasr

et al. 2019

BMC Complement Altern Med

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Background Bitter gourd ( Momordica charantia ) has attracted the focus of researchers owing to its excellent anti-diabetic action. The beneficial effect of Momordica charantia on heart has been reported by in vitro and in vivo studies. However the developmental toxicity or potential risk of M. charantia on fetus heart development is largely unknown. Hence this study was designed to find out the developmental toxicity of M. charantia using zebrafish ( Danio rerio ) embryos. Methods The crude extracts were prepared from fruit and seeds of M. charantia. The Zebrafish embryos were exposed to serial dilution of each of the crude extract. The biologically active fractions were fractionated by C18 column using high pressure liquid chromatography. Fourier-transform infrared spectroscopy and gas chromatography coupled with mass spectrophotometry was done to identify chemical constituents in fruit and seed extract of M. charantia . Results The seed extract of M. charantia was lethal with LD 50 values of 50 μg/ml to zebrafish embryos and multiple anomalies were observed in zebrafish embryos at sub-lethal concentration. However, the fruit extract was much safe and exposing the zebrafish embryos even to 200 μg/ml did not result any lethality. The fruit extract induced severe cardiac hypertrophy in treated embryos. The time window treatment showed that M. charantia perturbed the cardiac myoblast specification process in treated zebrafish embryos. The Fourier-transform infrared spectroscopy analyses revealed diverse chemical group in the active fruit fraction and five new type of compounds were identified in the crude seeds extract of M. charantia by gas chromatography and mass spectrophotometry. Conclusion The teratogenicity of seeds extract and cardiac toxicity by the fruit extract of M. charantia warned that the supplementation made from the fruit and seeds of M. charantia should be used with much care in pregnant diabetic patients to avoid possible damage to developing fetus. Electronic supplementary material The online version of this article (10.1186/s12906-019-2599-0) contains supplementary material, which is available to authorized users.

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“…As observed for many HMP used by the population, information regarding the safety of P. incarnata use during pregnancy and/or lactation is sparse (Veríssimo et al, 2011). To the best of our knowledge, only one study suggested, in vitro, an uterotonic action of this plant (Ruggy, Smith, 1940).…”

Section: Introductionmentioning

confidence: 99%

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams

Boll

Bortolasci

Zaminelli

et al. 2014

Braz. J. Pharm. Sci.

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Passiflora incarnata is marketed in many countries as a phytomedicine. Even though the directions of most marketed phytomedicines recommend them to be used under medical supervision, reproductive and developmental studies are sparse and not mandatory for regulatory purposes. In this study, a reproductive toxicity evaluation of P. incarnata was conducted in Wistar rats gavaged (30 or 300 mg/kg) during pregnancy and lactation. Moreover, considering that antioxidant properties have been attributed to flavonoids present in the genus Passiflora, it was also evaluated the antioxidant/pro-oxidant balance in the plasma of these dams and the antioxidant potential in an in vitro test. P. incarnata treatment did not influence dams´ body weight as well as reproductive (gestation length, post-implantation loss, litter size, litter weight) and hepatic (albumin, AST, ALT, GGT) parameters. The antioxidant property of P. incarnata was evidenced both in vivo (increase in the total antioxidant plasmatic potential) and in vitro (decrease in neutrophil-induced respiratory burst). The results from the present study indicate that under the experimental conditions evaluated, P. incarnata treatment during gestation and lactation presented antioxidant activity in the absence of maternal reproductive toxicity.Uniterms: Phytomedicines/evaluation. Passiflora incarnata/use in pregnancy. Passiflora incarnata/ use in lactation. Passiflora incarnata/reproductive toxicity/experimental study. Pregnancy/use of phytomedicines. Lactation/use of phytomedicines. Oxidative stress.Passiflora incarnata é comercializada em muitos países como fitoterápico. Embora a bula da maioria dos fitoterápicos recomende que eles sejam usados sob supervisão médica, estudos sobre a toxicidade reprodutiva e do desenvolvimento desses produtos são raros e não obrigatórios para fins regulatórios. Neste estudo, realizamos uma avaliação da toxicidade reprodutiva da P. incarnata, administrada a ratas Wistar (30 ou 300 mg/kg, gavagem) durante a gestação e a lactação. Além disso, considerando as propriedades antioxidantes que têm sido atribuídas aos flavonoides presentes no gênero Passiflora, também avaliou-se o equilíbrio antioxidante/pró-oxidante no plasma destas fêmeas e conduziu-se um teste in vitro para avaliar o potencial antioxidante. O tratamento com P. incarnata não influenciou o peso corporal das fêmeas, bem como indicadores de toxicidade reprodutiva (perdas pós-implantação, número de filhotes vivos e peso da ninhada) e os parâmetros de função hepática (albumina, AST, ALT, GGT). A propriedade antioxidante da P. incarnata foi evidenciada tanto in vivo (aumento do potencial antioxidante total plasmático) quanto in vitro (diminuição do burst respiratório em neutrófilos). Os resultados deste estudo indicam que, nas condições experimentais avaliadas, o tratamento com P. incarnata durante a gestação e lactação apresentou efeito antioxidante, na ausência de toxicidade reprodutiva materna.Unitermos: Fitoterápicos/avaliação. Passiflora incarnata/uso na gestação. Passiflora inca...

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Herbs of interest to the Brazilian Federal Government: female reproductive and developmental toxicity studies

Cited by 10 publications

References 39 publications

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Bitter gourd (Momordica charantia) possess developmental toxicity as revealed by screening the seeds and fruit extracts in zebrafish embryos

Passiflora incarnata treatment during gestation and lactation: toxicological and antioxidant evaluation in wistar dams

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