Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling

Arimura, Gen Ichiro; Garms, Stefan; Maffei, Massimo E.; Bossi, Simone; Schulze, B.; Leitner, Margit; Mithöfer, Axel; Boland, Wilhelm

doi:10.1007/s00425-007-0631-y

Cited by 132 publications

(134 citation statements)

References 47 publications

(61 reference statements)

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“…Induced biosynthesis of terpenoid volatiles in Arabidopsis and other plant species is dependent on JA signaling (Ament et al, 2006;Arimura et al, 2008;Attaran et al, 2008;Herde et al, 2008). By determining pathogen-induced MeSA emission from different Arabidopsis JA pathway mutants, we tested whether P. syringae-induced MeSA production would also require JA biosynthesis or associated downstream signaling events.…”

Section: Ja Signaling Regulates Mesa Production But Not Sarmentioning

confidence: 99%

Methyl Salicylate Production and Jasmonate Signaling Are Not Essential for Systemic Acquired Resistance inArabidopsis

et al. 2009

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Systemic acquired resistance (SAR) develops in response to local microbial leaf inoculation and renders the whole plant more resistant to subsequent pathogen infection. Accumulation of salicylic acid (SA) in noninfected plant parts is required for SAR, and methyl salicylate (MeSA) and jasmonate (JA) are proposed to have critical roles during SAR long-distance signaling from inoculated to distant leaves. Here, we address the significance of MeSA and JA during SAR development in Arabidopsis thaliana. MeSA production increases in leaves inoculated with the SAR-inducing bacterial pathogen Pseudomonas syringae; however, most MeSA is emitted into the atmosphere, and only small amounts are retained. We show that in several Arabidopsis defense mutants, the abilities to produce MeSA and to establish SAR do not coincide. T-DNA insertion lines defective in expression of a pathogen-responsive SA methyltransferase gene are completely devoid of induced MeSA production but increase systemic SA levels and develop SAR upon local P. syringae inoculation. Therefore, MeSA is dispensable for SAR in Arabidopsis, and SA accumulation in distant leaves appears to occur by de novo synthesis via isochorismate synthase. We show that MeSA production induced by P. syringae depends on the JA pathway but that JA biosynthesis or downstream signaling is not required for SAR. In compatible interactions, MeSA production depends on the P. syringae virulence factor coronatine, suggesting that the phytopathogen uses coronatine-mediated volatilization of MeSA from leaves to attenuate the SA-based defense pathway.

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Section: Ja Signaling Regulates Mesa Production But Not Sarmentioning

confidence: 99%

Methyl Salicylate Production and Jasmonate Signaling Are Not Essential for Systemic Acquired Resistance inArabidopsis

et al. 2009

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“…Jasmonates are key regulators of plant responses to damage, necrotrophic pathogens, and insect herbivory, whereas E modulates the magnitude of direct and indirect plant defenses produced in response to jasmonates (16,(22)(23)(24)(25)(26)(27)(28). SA is involved in systemic acquired resistance to biotrophic pathogens; however, its role in herbivore-induced responses is less clear (24,29).…”

mentioning

confidence: 99%

Phytohormone-based activity mapping of insect herbivore-produced elicitors

Schmelz

Engelberth

Alborn

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

229

225

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In response to insect attack, many plants exhibit dynamic biochemical changes, resulting in the induced production of direct and indirect defenses. Elicitors present in herbivore oral secretions are believed to positively regulate many inducible plant defenses; however, little is known about the specificity of elicitor recognition in plants. To investigate the phylogenic distribution of elicitor activity, we tested representatives from three different elicitor classes on the time course of defense-related phytohormone production, including ethylene (E), jasmonic acid (JA), and salicylic acid, in a range of plant species spanning angiosperm diversity. All families examined responded to at least one elicitor class with significant increases in E and JA production within 1 to 2 h after treatment, yet elicitation activity among species was highly idiosyncratic. The fatty-acid amino acid conjugate volicitin exhibited the widest range of phytohormone and volatile inducing activity, which spanned maize (Zea mays), soybean (Glycine max), and eggplant (Solanum melongena). In contrast, the activity of inceptinrelated peptides, originally described in cowpea (Vigna unguiculata), was limited even within the Fabaceae. Similarly, caeliferin A16:0, a disulfooxy fatty acid from grasshoppers, was the only elicitor with demonstrable activity in Arabidopsis thaliana. Although precise mechanisms remain unknown, the unpredictable nature of elicitor activity between plant species supports the existence of specific receptor-ligand interactions mediating recognition. Despite the lack of an ideal plant model for studying the action of numerous elicitors, E and JA exist as highly conserved and readily quantifiable markers for future discoveries in this field.ethylene ͉ insect elicitor ͉ jasmonic acid ͉ plant defense ͉ volatile organic compound

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“…Three putative sesquiterpenoid synthases, TPS1, TPS3 and TPS5, were therefore subcloned into the pHis8-3 expression vector and the recombinant vectors were transformed into Escherichia coli BL21-Codon Plus (DE3). [9] Interestingly, the crude cell lysates of all three enzymes converted geranyl-(GDP), farnesyl-(FDP) and geranylgeranyl diphosphate (GGDP) into terpenoids, albeit with diff erent efficiency. TPS1 generated (E)-β-caryophyllene and α-humulene from FDP and traces of myrcene, terpinolene and limonene from GDP.…”

mentioning

confidence: 99%

“…TPS5 generated 28 different products from FDP but only myrcene, terpinolene and limonene from GDP. [9] To understand to what extent the enzymes control the reaction pathways, the stereochemical course of product formation of the multiproduct sesquiterpene synthase MtTPS5 from Medicago truncatula was analysed in detail. Incubation of the recombinant MtTPS5 with FDP provided 18 sesquiterpene hydrocarbons and 10 sesquiterpene alcohols, with very diff erent carbon skeletons comprising the germacrane, cubebane and alloaromadendrene skeletons (Figure 2).…”

mentioning

confidence: 99%

“…For example, MtTPS5 produces optically pure (-)-β-cubebene and (-)-germacrene D. Similarly, MtTPS3 generates pure (3S)-nerolidol [9] and MtTPS1 (-)-(E)-β-caryophyllene. The high ee of the products of MtTPS5 is remarkable, since this demonstrates that the whole cascade between the initial ionization of FDP and the fi nal products is under tight control of the enzyme.…”

mentioning

confidence: 99%

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Induced volatiles of Medicago truncatula: molecular diversity and mechanistic aspects of a multiproduct sesquiterpene synthase from M. truncatula

Boland

Garms

2010

Flavour & Fragrance J

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Herbivore-induced terpenoid emission in Medicago truncatula: concerted action of jasmonate, ethylene and calcium signaling

Cited by 132 publications

References 47 publications

Methyl Salicylate Production and Jasmonate Signaling Are Not Essential for Systemic Acquired Resistance inArabidopsis

Methyl Salicylate Production and Jasmonate Signaling Are Not Essential for Systemic Acquired Resistance inArabidopsis

Phytohormone-based activity mapping of insect herbivore-produced elicitors

Induced volatiles of Medicago truncatula: molecular diversity and mechanistic aspects of a multiproduct sesquiterpene synthase from M. truncatula

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