Herbicide-tolerant Transgenic Pineapple (Ananas comosus) Produced by Microprojectile Bombardment

Sripaoraya, S.; Marchant, R.; Power, J. B.; Davey, M. R.

doi:10.1006/anbo.2001.1502

Cited by 35 publications

(32 citation statements)

References 18 publications

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“…Harvest of a second crop following replanting of the crowns of these fruit has produced normal fruit. These data indicate that somaclonal variation is unlikely to pose a significant problem in the long term with this transformation system, contrary to what was suggested by Sripaoraya et al (2001). The ppo and gus transformants are currently being assessed in five field trials for gene expression, genetic integrity and organoleptic characteristics.…”

Section: Genetic Integrity Of Transgenic Plantscontrasting

confidence: 67%

“…Microprojectile bombardment is one method of gene introduction that has been successful with several species. Publications by Becker et al (2000), Bower et al (1996), Brettell and Murray (1995), Klein et al (1992), Sripaoraya et al (2001), Vasil et al (1992) illustrate the wide range of crops which have been successfully transformed by this method.…”

Section: Introductionmentioning

confidence: 98%

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The Introduction of Transgenes to Control Blackheart in Pineapple (Ananas Comosus L.) cv. Smooth Cayenne by Microprojectile Bombardment

Ko¹,

Campbell²,

Jobin-Decor³

et al. 2006

Euphytica

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A transformation technique for the introduction of transgenes to control blackheart by particle bombardment has been developed for pineapple cv. Smooth Cayenne. Leaf callus cultures capable of high frequency organogenesis with a short regeneration time were used as explant material. Gus and gfp reporter genes were used to observe and determine transient and stable expression. The ppo gene, isolated from pineapple, was introduced to control blackheart. Co-transformation occurred with constructs containing the nptII gene conferring geneticin resistance. We have recovered 15 independent transgenic gus and gfp lines each from 8 separate experiments and 22 ppo lines from 11 experiments. Gus, gfp, ppo and nptII positive plants have been regenerated, which have been shown by Southern blot analysis to be stable transgenics containing multiple copies of the introduced genes. These results show that biolistic gene delivery in pineapple can be successfully achieved at an acceptable efficiency of 0.21-1.5% for genetic improvement of 'Smooth Cayenne', the industry standard throughout the world.

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Section: Genetic Integrity Of Transgenic Plantscontrasting

confidence: 67%

Section: Introductionmentioning

confidence: 98%

The Introduction of Transgenes to Control Blackheart in Pineapple (Ananas Comosus L.) cv. Smooth Cayenne by Microprojectile Bombardment

Ko¹,

Campbell²,

Jobin-Decor³

et al. 2006

Euphytica

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“…Several transgenic plants have been obtained by particle bombardment using the bar gene to generated herbicide resistance, such as dry beans (Aragão et al 2002), pineapple (Sripaoraya et al 2001), sugarcane (Falco et al 2000), wheat (Rasco-Gaunt et al 1999), papaya (Cabrera-Ponce et al 1995), rice (Cao et al 1992), among others but not in Coffea. In this report, we describe a simple procedure using particle bombardment to introduce the bar gene that confers resistance to ammonium glufosinate herbicide into embryogenic calli of Coffea canephora with subsequent recovery of transgenic coffee plants.…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation of Coffea canephora by particle bombardment

Ribas¹,

Kobayashi²,

Pereira³

et al. 2005

Biol. plant.

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Stable transformation of Coffea canephora P. was obtained by particle bombardment of embryogenic tissue. Leaf explants were cultured on medium supplemented with 5 μM isopentenyl-adenosine to induce direct embryogenesis. Explants with somatic embryos were transferred to half strength MS medium with 9 μM 2,4 dichlorophenoxyacetic acid. After 2 weeks, the explants with somatic embryos and embryogenic tissue were bombarded with tungsten particles (M-25) carrying the plasmid pCambia3301 (containing the bar and uidA genes) using a high pressure helium microprojectile device. The bombarded explants were submitted to selection on medium containing 5 μM ammonium glufosinate herbicide as selective agent. After 6 months, putative transgenic embryos were transferred to a growth regulator-free medium for germination. The regenerated plantlets were β-glucuronidase (GUS) positive whereas no GUS activity was observed in non-transgenic controls. Incorporation of the bar gene into the genome was confirmed by PCR and Southern blot analysis of the regenerated transformed plants. Greenhouse grown transgenic coffee plants were found to withstand the recommended level of the herbicide Finale™ for weed control.

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“…Herbicide tolerance Sripaoraya et al, (2001) Plants sprayed with Basta ™ @1400 mg/L remained healthy and retained their pigmentation. The generation of herbicidetolerant pineapple will facilitate more efficient weed control in this widely cultivated tropical crop.…”

Section: Pineapple (Ananascomosus)mentioning

confidence: 99%