Herbicide Roundup shows toxic effects in nontarget organism Drosophila

Abstract

Glyphosate‐based herbicide Roundup, as the most employed herbicide used for multiple purposes in agriculture, adversely affects nontarget organisms. We tested the effects of Roundup applied at larval and adult stages. Roundup caused developmental delay and increased larvae mortality. Roundup treatment reduced hemolymph glucose and glycogen levels in adult flies of both sexes at the highest concentration tested. Sex‐dependent diverse effects were found in catalase and Cu,Zn superoxide dismutase (Cu,Zn‐SOD) activities. Decreased aconitase activity, contents of thiols, and lipid peroxides were found after larval Roundup exposure. Furthermore, chronic exposure to adult flies decreased appetite, body weight, and shortened lifespan. Thus, our results suggest that high concentrations of Roundup are deleterious to both larvae and adults, resulting in a shift of the metabolism and antioxidant defense system in Drosophila melanogaster.

Limited effects of glyphosate on the behaviour and immunity of males from six populations of the European earwig

“…Over the past decade, a growing body of evidence has shown that the spraying of these chemical substances on and off crops can affect not only the target plants and animals but also a wide range of non-target organisms, including insects (Desneux et al 2007). This involuntary exposure to crop protection products, even at low and sublethal doses, often alters a wide diversity of parameters in the biology of non-target insects, including their physiology, immunity, behavior, and reproduction (Pereira et al 2018;Strilbytska et al 2022). As a result, the widespread and increasing use of these chemical substances around the world is expected to have major impacts on the ecology, tness and population dynamics of many insect species and, given the many ecosystem services they provide, on the functioning of most ecosystems (Schowalter et al 2018).…”

“…al. 2021;Kanabar et al 2021) and equivalent or even lower doses of glyphosate(Straw et al 2021;Kanabar et al 2021;Strilbytska et al 2022). These apparent lack of effects were also surprising given that we used a formulation of glyphosate (Roundup©), which is known to trigger more toxic changes in insect physiology and behaviour than the active molecule alone(Straw et al 2021;Strilbytska et al 2022).…”

“…2021;Kanabar et al 2021) and equivalent or even lower doses of glyphosate(Straw et al 2021;Kanabar et al 2021;Strilbytska et al 2022). These apparent lack of effects were also surprising given that we used a formulation of glyphosate (Roundup©), which is known to trigger more toxic changes in insect physiology and behaviour than the active molecule alone(Straw et al 2021;Strilbytska et al 2022). A possible explanation for our ndings is that boldness, aggregation, immunocompetence and immune investment (but not on general activity) are independent of glyphosate-induced changes in the microbiota of the European earwig.…”

Ecotoxicological effects of a glyphosate-based herbicide on Gryllus (Gryllus) assimilis (Orthoptera: Gryllidae) ontogeny: A study on antioxidant system, oxidative stress and cholinergic system

“…Khare et al 2019), being more expressive during the nymphal stage. This enzyme was also more relevant in the larval stage of the y Drosophila melanogaster, however, it was increased by exposure to a GBH 10 g/L(Strilbytska et al 2022), indicating that in different insect species the CAT activation can reduce levels of oxidative damage and extend lifespan(Giraldo et al 2021). Embryonic and nymphal Gryllus development is characterized by an elevated metabolism of ecdysteroids (molting hormones), cytochrome P450s (enzymes involved in substrate oxidation)(Berdan et al 2016).…”