Herbicide Metabolism and Cross-Tolerance in Transgenic Potato Plants Co-Expressing Human CYP1A1, CYP2B6, and CYP2C19

Inui, Hideyuki; Kodama, Takuya; Ohkawa, Yasunobu; Ohkawa, Hideo

doi:10.1006/pest.1999.2454

Cited by 53 publications

(40 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Desiree, with A. tumefaciens LBA 4404, according to Inui et al (2000). The plasmid pBE2113 Not I (Mitsuhara et al 1996) contained the neomycin phosphotransferase II (NPT II) gene as a selectable marker (conferring resistance to NPT II or kanamycin) under the control of the NOS promoter and a binary vector containing the rd29A promoter and DREB1A cDNA (Kasuga et al 1999).…”

Section: Methodsmentioning

confidence: 99%

The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

Behnam

Kikuchi

Celebi-Toprak

et al. 2006

Plant Biotechnology

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

Behnam

Kikuchi

Celebi-Toprak

et al. 2006

Plant Biotechnology

View full text Add to dashboard Cite

“…Desiree, and A. tumefaciens, LBA4404 according to the method of Inui et al (2000). The binary vector contained the rd29A promoter and a DREB1A cDNA clone (Kasuga et al 1999) that was constructed in pBE2113 at the Not I site (Mitsuhara et al 1996).…”

Section: Production and Identification Of Transgenic Linesmentioning

confidence: 99%

Tolerance to Salt Stress of the Transgenic Tetrasomic Tetraploid Potato, Solanum tuberosum cv. Desiree Appears to be Induced by the DREB1A Gene and rd29A Promoter of Arabidopsis thaliana

et al. 2005

View full text Add to dashboard Cite

“…tobacco and potato, was shown to enhance the oxidative metabolism of e.g. herbicides (Inui et al, 2000(Inui et al, , 2001 ). In these experiments, co-expression of mammalian NADPH-P450 reductase was not necessary, since the foreign P450s were supplied with the requisite electrons by endogenous plant reductases.…”

Section: Introductionmentioning

confidence: 99%

Metabolism of 4-n-Nonylphenol by Non-modified and CYP1A1- and CYP1A2-Transgenic Cell Cultures of Tobacco

Berger

Russ

Schuphan

et al. 2005

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

The metabolism of 14 C-4-n-nonylphenol ( 14 C-4-n-NP), as a model for the xenoestrogen nonylphenol, was investigated in three types of tobacco cell suspension cultures: one genetically non-modified culture (NT) and two cultures constitutively expressing human cytochrome P450 CYP1A1 or CYP1A2. With 1 mg l Ð1 of 14 C-4-n-NP and 24 h of incubation, the xenobiotic was transformed almost completely to glycosides. After glycosidic cleavage, 14 C-4-n-NP and several primary metabolites of 14 C-4-n-NP were liberated. Portions of the primary metabolites were 29.3% (NT culture), 34.3% (CYP1A1 culture), and 50.7% of applied 14 C (CYP1A2 culture). Thus, the endogenous capacity of the tobacco cells to form primary metabolites of 4-n-NP was noticeably higher than that of CYP1A1 or CYP1A2. The results however clearly suggest that 4-n-NP is Ð even though a poor Ð substrate of CYP1A1 and CYP1A2. In order to examine metabolic profiles of 4-n-NP in the NT, CYP1A1 and CYP1A2 cultures, the suspensions were exposed to 10 mg l Ð1 of 14 C-4-n-NP using a two-liquid-phase system with carrier n-hexadecane and 192 h of incubation. Results obtained resembled those of the low concentration study. The oxidative metabolic profiles determined after hydrolytic cleavage using GC-EIMS were similar in the NT, CYP1A1 and CYP1A2 cultures. Main metabolites were side-chain mono-hydroxylated derivatives of 4-n-NP with 6Ј-, 7Ј-and 8Ј-OH-4-n-NP as prominent metabolites. In addition, olefinic side-chain hydroxy, ring methoxylated, keto and ring hydroxylated derivatives were observed. The lack of differences in metabolic profiles among the CYP1A1, CYP1A2 and NT cultures was referred to the low enzymatic activity of CYP1A1 and CYP1A2 as compared to the higher endogenous oxidative capacity of tobacco, as well as to similar metabolic profiles of 4-n-NP produced by CYP1A1 and CYP1A2 and tobacco itself.

show abstract

Herbicide Metabolism and Cross-Tolerance in Transgenic Potato Plants Co-Expressing Human CYP1A1, CYP2B6, and CYP2C19

Cited by 53 publications

References 23 publications

The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

The Arabidopsis DREB1A gene driven by the stress-inducible rd29A promoter increases salt-stress tolerance in proportion to its copy number in tetrasomic tetraploid potato (Solanum tuberosum)

Tolerance to Salt Stress of the Transgenic Tetrasomic Tetraploid Potato, Solanum tuberosum cv. Desiree Appears to be Induced by the DREB1A Gene and rd29A Promoter of Arabidopsis thaliana

Metabolism of 4-n-Nonylphenol by Non-modified and CYP1A1- and CYP1A2-Transgenic Cell Cultures of Tobacco

Contact Info

Product

Resources

About