Herbicide Metabolism and Cross-Tolerance in Transgenic Potato Plants Expressing Human CYP1A1

A selectable marker using cytochrome P450 monooxygenases for Arabidopsis transformation

“…CYP1A1 and CYP2C19 showed similar substrate specificity, but CYP2B6 was different. This result is similar to that in transgenic potato plants expressing these P450 cDNAs (Inui et al 1999). Selection with norflurazon was an easy system to distinguish transformants from non-transformants because of bleaching of leaves, but most selection methods gave appreciable differences in growth.…”

“…The introduction into plants of highly active mammalian P450 species that metabolize herbicides provides not only herbicide tolerance, but also selectable markers. Human P450 species have been used to generate herbicide-tolerant tobacco, potato, and rice plants (Shiota et al 1994;Inui et al 1999;Inui et al 2001a). As these transgenic plants carrying human P450 species tolerate many herbicides, this selection system can be used to construct a system for selection of transgenic plants by using herbicides.…”

Enhancement of metabolizing herbicides in young tubers of transgenic potato plants with the rat CYP1A1 gene

“…They also reported that the N-dealkylation was mainly induced by rat P450 species CYP1A1, CYP1A2, CYP2B1 and CYP2B2, and deisopropylation by CYP2B1 and CYP2B2 (Hanioka et al 1999). Inui et al (1999) reported that transgenic potato plants harboring the human CYP1A1 gene metabolized atrazine to detoxified compounds through deisopropylation and then N-dealkylation. The amount of residual atrazine in the micro-tubers of Ag2197 plants harboring the patatin-CYP1A1 gene decreased until it was about onehalf of that found in the micro-tubers of May Queen plants (Fig.…”

Herbicide Tolerant Food Legume Crops: Possibilities and Prospects

“…There are recent reports using non-prokaryotic genes to confer resistance, but none are yet commercialized, and whether they confer sufficient resistance is not clear. The examples include a rabbit esterase gene conferred resistance to thiazopyr via degradation (Feng et al, 1998), the expression of plant and animal P450 transgenes conferred phenyl urea resistance (Inui et al, 1999;Siminsky et al, 1999). Transgenes encoding maize glutathione transferases increased the level of herbicide resistance .…”