Herbicide Effects on Liposome Leakage

Abstract

Herbicide effects on chromate leakage from liposomes prepared from crude soybean [Glycine max(L.) Merr.] phosphatidylcholine were determined over a concentration range of 2.5 × 10-5to 5.0 × 10-3M. Dye levels in the dialysate were measured spectrophotometrically (370 nm) after 1 h. Barban (4-chloro-2-butynyl-m-chlorocarbanilate) at 2.5 to 5.0 × 10-4M and PCP (pentachlorophenol) at 0.5 to 1.0 × 10-3M induced significant leakage. MCPB {4-[(4-chloro-o-tolyl)oxy] butyric acid}, 2,4,5-T [(2,4,5-trichlorophenoxy)acetic acid], and ioxynil (4-hydroxy-3,5-diiodobenzonitrile), all at 2.5 × 10-3M, also caused dye leakage, but metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide] induced dye leakage only at 3.0 to 5.0 × 10-3M. Nitrofen (2,4-dichlorophenyl-p-nitrophenyl ether) and atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] were least effective over the concentration range tested.

Partition of 2, 4-Dichiorophenoxyacetic Acid Derivatives in Phosphatidyicholine Multi-lamellar Vesicles

“…The similar observations have been reported for 2, 4-D and its analogs. 3,4,[7][8][9][10] The pKa values of these acids range from 2. 8 to 5.…”