Herbal Mixture Adsorbed to Polyethylene Glycol Microspheres Induces Apoptotic Effects on Breast Cancer Cells

Ribeiro, Aliny Aparecida Lopes; Silva, Fabiana Helen da; Cotrim, Aron Carlos de Melo; Deluque, Alessandra Lima; Marchi, Patrícia Gelli Feres de; Fagundes, Danny Laura Gomes; Pereira, Claudia de Sousa; França, Eduardo Luzía; Honório-França, Adenilda Cristina

doi:10.2174/1567201814666171002141430

Cited by 9 publications

(7 citation statements)

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“…In the present study, the interaction of PEG microspheres with blood phagocytes co-cultured or not with MCF-7 cells did not affect the viability of the cells tested, suggesting that HSEE is non-toxic. Other plant extracts have shown a similar non-toxic response (Scherer et al, 2011;Reinaque et al, 2012;Cortês et al, 2013;Ribeiro et al, 2018). In fact, plant extracts can activate the immune system to help in the treatment of diseases without compromising the viability of the immune cells involved (Hasani-Ranjbar et al, 2008).…”

Section: Discussionmentioning

confidence: 96%

“…Co-culture of MNand MCF-7 cells Cell co-culture systems incorporating MN and MCF-7 cells (5x10 6 cells.mL -1 ) were performed using the same protocol described by Ribeiro et al, (2018).…”

Section: Cell Viabiltymentioning

confidence: 99%

“…The combination of medicinal plants and polymer matrices raises the possibility of developing new drugs and treatments. For instance, a recent study found that a mixture of plant extracts adsorbed onto PEG microspheres have antitumor effects, and the authors suggested that the participation of blood mononuclear (MN) cells is important in achieving that result (Ribeiro et al, 2018). The Brazilian Cerrado is the second largest biome in South America and the richest tropical biome in the world in terms of biodiversity (Sano et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Araújo¹,

Savazzi²,

Fujimori³

et al. 2019

Asian Pac J Cancer Prev

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Objective: To evaluate the antitumor effects of polyethylene glycol (PEG) microspheres with adsorbed Hancornia speciosa ethanolic extract (HSEE) on blood mononuclear (MN) cells co-cultured with MCF-7 breast cancer cells. Methods: PEG microspheres were adsorbed with HSEE and examined by flow cytometry and fluorescence microscopy. MCF-7 and MN cells obtained from volunteer donors were pre-incubated alone or co-cultured (MN and MCF-7 cells) for 24 h with or without HSEE, PEG microspheres or PEG adsorbed with HSEE (PEG-HSEE). Cell viability, superoxide release and superoxide dismutase were determined. Results: Fluorescence microscopy showed that PEG microspheres were able to absorb HSEE throughout their surface. Irrespective of the treatment, the viability index of MN cells, MCF-7 and their co-culture was not affected. Superoxide release increased in co-cultured cells treated with HSEE, adsorbed or not onto PEG microspheres. In co-cultured cells, SOD levels in culture supernatant increased in the treatment with HSEE, adsorbed onto PEG microspheres or not. Conclusion: HSEE has direct effects on MN cells co-cultured with MCF-7 cells. The results suggest the benefits of Hancornia speciosa fruit consumption by women at risk of breast cancer. In addition, because PEG-HSEE maintained oxidative balance in co-cultured cells, it is a promising alternative for the treatment of tumor cells.

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Section: Discussionmentioning

confidence: 96%

“…Co-culture of MNand MCF-7 cells Cell co-culture systems incorporating MN and MCF-7 cells (5x10 6 cells.mL -1 ) were performed using the same protocol described by Ribeiro et al, (2018).…”

Section: Cell Viabiltymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Araújo¹,

Savazzi²,

Fujimori³

et al. 2019

Asian Pac J Cancer Prev

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“…The collection of colostrum from the lactating participants was performed following the protocol developed by França et al [6]. Two breast milk samples were collected [ [26] were performed using the same protocol described above.…”

Section: Obtaining Colostrum Samples and Colostrum Cells Separationmentioning

confidence: 99%

Nanodoses of melatonin induces apoptosis on human breast cancer cells co-cultured with colostrum cells

2019

Biointerface Res Appl Chem

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The aim of this study was to investigate the effect of melatonin nanodoses adsorbed to PEG microspheres during different phases of the day on oxidative stress and apoptosis in human colostrum mononuclear (MN) cells co-cultured with breast cancer tumor cells (MCF-7). The MCF-7 cells were obtained from the American Type Culture Collection, and the MN cells were obtained from volunteer donors. The cells were preincubated for 24 h with or without 100 ng.mL-1 melatonin (MLT), PEG microspheres (PEG) or 100 ng.mL-1 MLT adsorbed to PEG microspheres (PEG-MLT). Superoxide, superoxide dismutase (SOD), intracellular calcium and apoptosis in the MN cells, MCF-7 cells and co-cultures were determined. The MN cells and co-cultured cells during the nocturnal period and the MCF-7 cells during the diurnal period exhibited increased superoxide release in the presence of PEG-MLT. MN cells treated with MLT during both periods of the day exhibited the highest SOD concentrations, whereas the MCF-7 cells had high SOD levels when incubated with PEG-MLT during the nocturnal period. The nocturnal period co-culture, independent of treatment, showed the highest levels of the enzyme. The highest amount of intracellular Ca2+ release was observed in MN cells and MCF-7 cells co-cultured with PEG-MLT during the nocturnal period. Irrespective of the phase of the day, the highest apoptosis index was observed in co-cultures of cells incubated with MLT-PEG. These data suggest that nanodoses of melatonin-modified release plus physiological melatonin produced in higher concentrations during the night can increase the effective activity of this hormone against tumors.

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“…Studies using hormones such as melatonin and secretory IgA antibodies extracted from human milk adsorbed onto PEG microspheres showed that the controlled release of this compound was able to reduce viability and induce apoptosis in MCF-7 cell lines [11,12]. Other herbicidal and barium chloride studies, adsorbed to PEG microspheres on human blood mononuclear cells co-cultured with breast cancer cell lines (MCF-7), showed a pro-apoptotic effect in breast cancer cells MCF-7 human [94,95]. Immunotherapy for tumor treatments based on cytotoxic properties of immunocompetent cells has also been the focus of many studies.…”

Section: Polyethylene Glycol (Peg) Andmentioning

confidence: 99%

Melatonin bioengineered: A New Possible Strategy for Treatment of Breast Cancer

Fernandes¹,

Cotrim²,

França³

et al. 2018

IJAERS

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Breast cancer is an important public health problem, with an estimated 3.2 million new cases by the year 2050. Diet plays a key role in the etiology of breast cancer and breastfeeding is associated with a lower incidence of breast cancer. On the other hand, the improvement of the therapeutic properties of bioactive compounds through their incorporation into microcarriers is an important strategy in obtaining new therapies, since cyclical changes in concentration are eliminated; there is biological availability of the compound as well as the reduction in toxicity, number dose and suppression of adverse reactions. Studies using hormones such as melatonin extracted from human milk adsorbed onto polyethylene glycol (PEG) microspheres showed that the controlled release of this compound was able to reduce viability and induce apoptosis in MCF-7 cell lines. Colostrum differs from most of the secretions because it contains viable leukocytes during the first days of lactation with a quantity and activity comparable to blood leukocytes, and has several defense components such as antibodies and hormones, such as melatonin (MLT). This review details the influence of the soluble and cellular components present in human colostrum, such as the MLT hormone, as the modified release systems influence the action of MLT and the possible mechanisms involved that contribute to the hypothesis of reduction of breast cancer in women who breastfed.

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Herbal Mixture Adsorbed to Polyethylene Glycol Microspheres Induces Apoptotic Effects on Breast Cancer Cells

Abstract: These data suggest that herbal mixture adsorbed by PEG microspheres has apoptotic effects on human MCF-7 breast cancer cells and is therefore a possible therapeutic alternative.

Cited by 9 publications

References 0 publications

Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Effects of Mangaba (Hancornia speciosa) Fruit Extract Adsorbed onto PEG Microspheres in MCF-7 Breast Cancer Cells Co-Cultured with Blood Cells

Nanodoses of melatonin induces apoptosis on human breast cancer cells co-cultured with colostrum cells

Melatonin bioengineered: A New Possible Strategy for Treatment of Breast Cancer

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