Herbal medicine against genotoxicity of dimethoate, an insecticide, in mammalian somatic cells

Abstract

In this study, the genotoxic effects of dimethoate (DIM) were investigated with the in vitro micronucleus test in human peripheral lymphocytes. The ethanol extracts of Rosa canina and Salvia lavandulifolia were used to remove possible genotoxic effects of these substances. For this purpose, different concentrations (0.5-1-2 μg/mL) of dimethoate, DIM + RCeta and DIM + SLeta (1:1 v/v) application groups were prepared and applied to the blood culture. The obtained data were compared with the negative control group that was prepared with dimethyl sulfoxide (DMSO) as solvent and a well-known genotoxic effects of ethyl methanesulfonate (EMS) as positive control group. It was observed in lymphocyte cells that the frequency of MN considerably increased depending on the increasing dose of DIM whereas the nuclear division index (NBI)decreased according to the control group, especially in the last concentration (2 μg/mL). But, as the MN frequency decreased, NBI values approached to control group with 2μg/mL DIM + RCeta and 2μg/mL DIM + SLeta according to DIM application group (P < 0.05). Additionally, RCeta and SLeta were analyzed by gas chromotography-mass spectrometry (GC-MS).

Binding and Detoxification of Insecticides by Potentially Probiotic Lactic Acid Bacteria Isolated from Honeybee (Apis mellifera L.) Environment—An In Vitro Study

“…Various pesticides demonstrate the ability to damage the genetic information of the cell [ 93 ]. According to Kizılet et al [ 94 ], dimethoate shows genotoxic effects on human peripheral lymphocytes and introduces genetic alterations even at low concentrations [ 94 ]. The degree of genotoxicity of some pesticides depends on the doses and the time of exposure [ 95 ].…”

Assessment of anilofos-induced mutagenicity in bone marrow and germ cells of Swiss albino mice

“…for 90 days. The significant increase in these test results is a measure of genotoxic effects (Bagri and Jain, 2019;Bagri et al, 2016;Das et al, 2007;Kizilet et al, 2019;Yahia and Ali, 2019). The MNT is used for cytogenetic screening and is an in vivo assay that detects freshly induced structural CAs and chromosomal loss due to partial destruction.…”