Herbal Formula Danggui-Shaoyao-San Promotes Neurogenesis and Angiogenesis in Rat Following Middle Cerebral Artery Occlusion

Ren, Chang­hong; Wang, Brian; Li, Ning; Jin, Kunlin; Ji, Xunming

doi:10.14336/ad.2014.1126

Cited by 35 publications

(27 citation statements)

References 35 publications

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“…However DSS has been shown to reduce infract size 24 h post-stroke in a mouse model of MCAO [ 111 ] and to increase cerebral blood flow in the hippocampus in a rat model of global cerebral ischemia [ 112 ]. In a recent study, Ren et al (2015) demonstrated that these post-ischemic protective effects of DSS are associated with focal angiogenesis and neurogenesis via up-regulation of VEGF protein expression and increased eNOS activity [ 113 ]. In a mouse model of ageing, DSS demonstrated a neuroprotective effect by ameliorating oxidative stress and neuronal apoptosis via the Bcl-2/Bax and caspase-3 signalling in the brain [ 114 ].…”

Section: Angiogenic Effects Of Chinese Herbal Medicine In Ischemicmentioning

confidence: 99%

Angiogenesis in Ischemic Stroke and Angiogenic Effects of Chinese Herbal Medicine

Seto

Chang

Jenkins

et al. 2016

JCM

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Stroke is one of the major causes of death and adult disability worldwide. The underlying pathophysiology of stroke is highly complicated, consisting of impairments of multiple signalling pathways, and numerous pathological processes such as acidosis, glutamate excitotoxicity, calcium overload, cerebral inflammation and reactive oxygen species (ROS) generation. The current treatment for ischemic stroke is limited to thromolytics such as recombinant tissue plasminogen activator (tPA). tPA has a very narrow therapeutic window, making it suitable to only a minority of stroke patients. Hence, there is great urgency to develop new therapies that can protect brain tissue from ischemic damage. Recent studies have shown that new vessel formation after stroke not only replenishes blood flow to the ischemic area of the brain, but also promotes neurogenesis and improves neurological functions in both animal models and patients. Therefore, drugs that can promote angiogenesis after ischemic stroke can provide therapeutic benefits in stroke management. In this regard, Chinese herbal medicine (CHM) has a long history in treating stroke and the associated diseases. A number of studies have demonstrated the pro-angiogenic effects of various Chinese herbs and herbal formulations in both in vitro and in vivo settings. In this article, we present a comprehensive review of the current knowledge on angiogenesis in the context of ischemic stroke and discuss the potential use of CHM in stroke management through modulation of angiogenesis.

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Section: Angiogenic Effects Of Chinese Herbal Medicine In Ischemicmentioning

confidence: 99%

Angiogenesis in Ischemic Stroke and Angiogenic Effects of Chinese Herbal Medicine

Seto

Chang

Jenkins

et al. 2016

JCM

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show abstract

“…61010; AM Bickford Inc., Wales Center, NY, USA) was used for induction and maintenance. Focal cerebral ischemia was generated using the intraluminal middle cerebral artery occlusion (MCAO) model as described previously [16,21]. Briefly, the right common carotid artery and the right external carotid artery (ECA) were exposed.…”

Section: Animalsmentioning

confidence: 99%

Limb Ischemic Perconditioning Attenuates Blood-Brain Barrier Disruption by Inhibiting Activity of MMP-9 and Occludin Degradation after Focal Cerebral Ischemia

Ren¹,

Li²,

Wang³

et al. 2015

Aging and disease

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Remote ischemic perconditioning (PerC) has been proved to have neuroprotective effects on cerebral ischemia, however, the effect of PerC on the BBB disruption and underlying mechanisms remains largely unknown. To address these issues, total 90 adult male Sprague Dawley (SD) rats were used. The rats underwent 90-min middle cerebral artery occlusion (MCAO), and the limb remote ischemic PerC was immediately applied after the onset of MCAO. We found that limb remote PerC protected BBB breakdown and brain edema, in parallel with reduced infarct volume and improved neurological deficits, after MCAO. Immunofluorescence studies revealed that MCAO resulted in disrupted continuity of claudin-5 staining in the cerebral endothelial cells with significant gap formation, which was significantly improved after PerC. Western blot analysis demonstrated that expression of tight junction (TJ) protein occludin was significantly increased, but other elements of TJ proteins, claudin-5 and ZO-1, in the BBB endothelial cells were not altered at 48 h after PerC, compared to MCAO group. The expression of matrix metalloproteinase (MMP-9), which was involved in TJ protein degradation, was decreased after PerC. Interestingly, phosphorylated extracellular signal-regulated kinase 1/2 (pERK1/2), an upstream of MMP-9 signaling, was significantly reduced in the PerC group. Our data suggest that PerC inhibits MMP-9-mediated occludin degradation, which could lead to decreased BBB disruption and brain edema after ischemic stroke.Key words: ischemic stroke, remote ischemic perconditioning, blood-brain barrier, tight junction, matrix metalloproteinasesThe blood-brain barrier (BBB) is a physical and metabolic interface that segregates the central nervous system (CNS) from the peripheral circulation in addition to regulating the CNS microenvironment [1]. Under physiological conditions, BBB integrity is maintained by endothelial cell through tight junctions (TJs) and the basal lamina, limiting paracellular movement of ions, solutes, and water [2]. Yet, TJs are key components of the BBB structure that seal the gaps between adjacent endothelial cells and restrict paracellular permeability [3]. The TJ proteins, occludin and claudins, are important molecules in forming this seal [4]. In vivo, cerebral ischemia induces claudin-5

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“…However, the time window for this treatment is limited, and the hemorrhage risk is high. 2 The development of a new strategy to increase blood supply to the ischemic area is necessary and urgent.…”

Section: Introductionmentioning

confidence: 99%

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Zhou

Wang

et al. 2019

CNS Neurosci Ther

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Summary Introduction Dl‐3‐N‐butylphthalide (NBP), a small molecule drug used clinically in the acute phase of ischemic stroke, has been shown to improve functional recovery and promote angiogenesis and collateral vessel circulation after experimental cerebral ischemia. However, the underlying molecular mechanism is unknown. Aims To explore the potential molecular mechanism of angiogenesis induced by NBP after cerebral ischemia. Results NBP treatment attenuated body weight loss, reduced brain infarct volume, and improved neurobehavioral outcomes during focal ischemia compared to the control rats ( P < 0.05). NBP increased the number of CD31 + microvessels, the number of CD31 + /BrdU + proliferating endothelial cells, and the functional vascular density ( P < 0.05). Further study demonstrated that NBP also promoted the expression of vascular endothelial growth factor and angiopoietin‐1 ( P < 0.05), which was accompanied by upregulated sonic hedgehog expression in astrocytes in vivo and in vitro. Conclusion NBP treatment promoted the expression of vascular endothelial growth factor and angiopoietin‐1, induced angiogenesis, and improved neurobehavioral recovery. These effects were associated with increased sonic hedgehog expression after NBP treatment. Our results broadened the clinical application of NBP to include the later phase of ischemia.

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Herbal Formula Danggui-Shaoyao-San Promotes Neurogenesis and Angiogenesis in Rat Following Middle Cerebral Artery Occlusion

Cited by 35 publications

References 35 publications

Angiogenesis in Ischemic Stroke and Angiogenic Effects of Chinese Herbal Medicine

Angiogenesis in Ischemic Stroke and Angiogenic Effects of Chinese Herbal Medicine

Limb Ischemic Perconditioning Attenuates Blood-Brain Barrier Disruption by Inhibiting Activity of MMP-9 and Occludin Degradation after Focal Cerebral Ischemia

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

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