Herba Cistanches stimulates cellular glutathione redox cycling by reactive oxygen species generated from mitochondrial respiration in H9c2 cardiomyocytes

Wong, Hoi Shan; Ko, Kam Ming

doi:10.3109/13880209.2012.710242

Cited by 30 publications

(35 citation statements)

References 35 publications

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“…After a 4 h incubation with BS at a final concentration of 10 μM, H9c2 cells were trypsinized and suspended in assay buffer (120 mM KCl, 5 mM KH 2 PO 4 , 2 mM EGTA, 10 mM HEPES, 0.1 MgCl 2 , 0.5% BSA, pH 7.4) and stored at 37°C. Mitochondrial respiration was measured using a Clarke-type electrode (Hansatech Instruments Ltd., NFK) as described (Sgobbo et al, 2007;Wong and Ko, 2013). In essence, an aliquot (1 mL) of suspended cells (1.5 × 10 5 cells/mL) was placed in an air tight liquid-phase oxygen electrode chamber.…”

Section: Methodsmentioning

confidence: 99%

β‐Sitosterol Enhances Cellular Glutathione Redox Cycling by Reactive Oxygen Species Generated From Mitochondrial Respiration: Protection Against Oxidant Injury in H9c2 Cells and Rat Hearts

Wong

Chen

Leong

et al. 2013

Phytotherapy Research

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Herba Cistanches (Cistanche deserticola Y. C. Ma) is a 'Yang-invigorating' tonic herb in Chinese medicine. Preliminary chemical analysis indicated that β-sitosterol (BS) is one of the chemical constituents in an active fraction of Herba Cistanches. To investigate whether BS is an active ingredient of Herba Cistanches, the effects of BS on H9c2 cells and rat hearts were examined. The results indicated that BS stimulated the mitochondrial ATP generation capacity in H9c2 cells, which was associated with the increased production of mitochondrial reactive oxygen species. BS also stimulated mitochondrial state 3 and state 4 respiration, with the resultant decrease in coupling efficiency. BS produced an up-regulation of cellular glutathione redox cycling and protected against hypoxia/reoxygenation-induced apoptosis in H9c2 cells. However, the protective effect of BS against myocardial ischemia/reperfusion injury was seen in female but not male rats ex vivo. The cardioprotection afforded by BS was likely mediated by an up-regulation of mitochondrial glutathione redox cycling in female rat hearts. In conclusion, the ensemble of results suggests that BS is an active ingredient of Herba Cistanches. The gender-dependent effect of BS on myocardial protection will further be investigated.

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Section: Methodsmentioning

confidence: 99%

β‐Sitosterol Enhances Cellular Glutathione Redox Cycling by Reactive Oxygen Species Generated From Mitochondrial Respiration: Protection Against Oxidant Injury in H9c2 Cells and Rat Hearts

Wong

Chen

Leong

et al. 2013

Phytotherapy Research

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“…Under the present experimental conditions, incubations with HCY2 or UA were both found to induce a time-dependent cyclic variation of cellular GSH levels in H9c2 cells, indicative of upregulation of glutathione redox cycling. As also observed in Herba Cistanches-treated H9c2 cells [36], the activation of glutathione redox cycling is presumably related to the interplay between HCY2/UA-induced reactive oxygen species (ROS) generation, an event secondary to the increased electron transport, and the GR-catalyzed and NADPH-mediated regeneration of GSH. Given that increased formation of ROS within mitochondria can cause an adaptive response mediated by the activation of nuclear factor-erythroid 2 p45-related factor 2 [37, 38], HCY2/UA may therefore positively regulate GR, which in turn catalyzes the regeneration of GSH from GSSG, leading to the cyclic change in cellular GSH levels.…”

Section: Discussionmentioning

confidence: 85%

Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling

Chen

2013

Evidence-Based Complementary and Alternative Medicine

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Mitochondrial decay is considered to be a major contributor to aging-related diseases, including neurodegenerative diseases, cardiovascular disorders, and certain metabolic diseases. Therefore, the maintenance of mitochondrial functional capacity and antioxidant status should play an essential role in preventive health. Herba Cynomorii, which is one of the most potent “Yang-invigorating” Chinese tonic herbs, was found to increase mitochondrial ATP generation capacity (ATP-GC) in rat hearts ex vivo. In the present study, we demonstrated that HCY2, an active fraction of Herba Cynomorii, and its major ingredient ursolic acid (UA) could protect against hypoxia/reoxygenation-induced cell apoptosis in H9c2 cells in vitro and also against ischemia/reperfusion-induced injury in rat hearts ex vivo. The cardioprotection was associated with an increase in ATP-GC and an enhancement of glutathione redox cycling. The results suggest that UA may be one of the active ingredients responsible for the cardioprotection afforded by Herba Cynomorii, and this effect may be mediated, at least in part, by enhancement of mitochondrial functional capacity and antioxidant status, possibly through the induction of mitochondrial uncoupling.

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“…É possível limitar os danos induzidos pelo estresse oxidativo prevenindo a progressão das lesões no tecido cardíaco através da tentativa de manter o equilíbrio entre a produção de ROS e da quantidade de antioxidantes endógenos, incluindo as enzimas superóxido dismutase (SOD), catalase (CAT), glutationa reduzida (GSH), glutationa peroxidase (GPx) e glutationa redutase (GRD), que tem como função remover o excesso de ROS 10,17,25 . Assim, a atividade das enzimas SOD, CAT, GSH, GPx e GRD foram avaliadas e foi encontrado um aumento significante na atividade destas enzimas 8,10,17,19,21,26 . As lesões no miocár-dio geradas pela lipoperoxidação foram reduzidas pelo tratamento prévio com produtos naturais, demonstrado através da quantificação da substância reativa ao ácido tiobarbitúrico (TBARS) 6,10,20,26 .…”

Section: Discussionunclassified

Revisão sistemática dos efeitos de substâncias naturais com ação antioxidante no tratamento da injúria de reperfusão após isquemia

Miguel-dos-Santos

Santos

2016

cmbio

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ResumoIntrodução: doenças isquêmicas do coração, especialmente, o infarto agudo do miocárdio, são as principais causas de morte no mundo, tanto em países desenvolvidos como em desenvolvimento. Objetivo: através de uma revisão sistemática, analisar o desempenho de extrato de plantas, com atividade antioxidante comprovada, sobre a lesão do miocárdio induzida pela reperfusão após a isquemia. Metodologia: a busca bibliográfica foi realizada na base de dados eletrônica Medline, em setembro de 2015. INTRODUÇÃODoenças isquêmicas do coração, especialmente o infarto agudo do miocárdio, são as principais causas de morte no mundo, tanto em países desenvolvidos como em desenvolvimento. É crucial para a sobrevivência do paciente, após um evento isquêmico, o rápido início da reperfusão. Entretanto, durante os primeiros períodos de reperfusão o miocárdio pode sofrer lesões, além daquelas geradas no período de isquemia 1 , denominadas como injúria de reperfusão 2 . As diferentes manifestações clínicas desta injúria incluem a necrose do miocárdio, apoptose, e arritmias do miocárdio 3 . A produção de espécies reativas de oxigênio (ROS), vista concomitantemente com a reperfusão, é considerada um dos fatores principais no desenvolvimento dos danos provocados no miocárdio 4 e pode aumentar a magnitude da lesão deste órgão submetido à isquemia 5 . Durante os eventos de isquemia e reperfusão no coração, as ROS desempenham importantes papéis na gênese da lesão no tecido cardíaco 6 . Na literatura é relatado que produtos naturais derivados de plantas podem desempenhar efeitos cardioprotetores, através de processos complexos e multifatoriais, e estes efeitos parecem depender, principalmente, de sua propriedade de neutralizar as ROS [6][7][8][9][10][11][12] . Além disso, diversos estudos tem demostrado que o tratamento com substâncias antioxidantes melhora a função ventricular pós-isquemia 13,14 . Desta forma, a presente revisão sistemática, teve como objetivo analisar o desempenho de produtos naturais derivados de plantas com atividade antioxidante comprovada, sobre a lesão do miocárdio induzida pela reperfusão após a isquemia.

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Herba Cistanches stimulates cellular glutathione redox cycling by reactive oxygen species generated from mitochondrial respiration in H9c2 cardiomyocytes

Cited by 30 publications

References 35 publications

β‐Sitosterol Enhances Cellular Glutathione Redox Cycling by Reactive Oxygen Species Generated From Mitochondrial Respiration: Protection Against Oxidant Injury in H9c2 Cells and Rat Hearts

β‐Sitosterol Enhances Cellular Glutathione Redox Cycling by Reactive Oxygen Species Generated From Mitochondrial Respiration: Protection Against Oxidant Injury in H9c2 Cells and Rat Hearts

Ursolic-Acid-Enriched Herba Cynomorii Extract Protects against Oxidant Injury in H9c2 Cells and Rat Myocardium by Increasing Mitochondrial ATP Generation Capacity and Enhancing Cellular Glutathione Redox Cycling, Possibly through Mitochondrial Uncoupling

Revisão sistemática dos efeitos de substâncias naturais com ação antioxidante no tratamento da injúria de reperfusão após isquemia

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