Heptylmannose-functionalized cellulose for the binding and specific detection of pathogenic <i>E. coli</i>

Cauwel, Madeleine; Sivignon, Adeline; Bridot, Clarisse; Nongbe, Medy C.; Deniaud, David; Roubinet, Benoît; Landemarre, Ludovic; Felpin, François‐Xavier; Bouckaert, Julie; Barnich, Nicolas; Gouin, Sébastien G.

doi:10.1039/c9cc05545b

Cited by 15 publications

(16 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To demonstrate the efficacity of such therapy in CD patients, AIEC-carriage should be considered as an essential criterion of inclusion. Use of a concomitant companion diagnostic test to rapidly guide patients towards an AIEC-targeted treatment is necessary and heptyl-mannose-grafted cellulose represents a promising method to easily detect AIEC in biological fluids (Cauwel et al, 2019). It should also be interesting to propose this soluble glucan as a post-biotic strategy in patients susceptible to be colonized by AIEC, for example after surgery to limit AIEC implantation at the ileal mucosa, in order to decrease postoperative recurrence and thus to extend periods of remission.…”

Section: Discussionmentioning

confidence: 99%

Heteropolysaccharides from S. cerevisiae show anti-adhesive properties against E. coli associated with Crohn's disease

Sivignon

Ballet

et al. 2021

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

The Saccharomyces cerevisiae CNCM I-3856 was previously reported to strongly inhibit adherent-invasive Escherichia coli (AIEC) adhesion to intestinal epithelial cells in vitro and to favor AIEC elimination from the gut in a murine model of Crohn's disease in vivo. In order to identify which cell wall components of yeast are responsible for AIEC elimination, constituent polysaccharides of yeast were isolated and their antiadhesive ability against AIEC adhesion in vitro were screened. A fraction containing mannan, b-glucan and a-glucan extracted from yeast cell-walls was shown to inhibit 95% of AIEC adhesion in vitro and was thus identified as the strongest anti-adhesive yeast cell wall component. Furthermore, this mannan-glucancontaining fraction was shown to accelerate AIEC decolonization from gut in vivo. This fraction could be proposed as a treatment to eliminate AIEC bacteria in patients with Crohn's disease, a microbial trigger of intestinal inflammation. Highlights• Mannan extracted from yeast inhibits E. coli adhesiveness to intestinal cells in vitro.• A soluble fraction containing b-glucan/a-glucan/mannan (GGM) was extracted from S. cerevisiae cell wall.• This soluble GGM fraction strongly inhibits E. coli adhesiveness to intestinal cells in vitro.• This soluble GGM fraction favors elimination of E. coli from gut in vivo.

show abstract

Section: Discussionmentioning

confidence: 99%

Heteropolysaccharides from S. cerevisiae show anti-adhesive properties against E. coli associated with Crohn's disease

Sivignon

Ballet

et al. 2021

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

show abstract

“…Heptylmannoside derivatives are shown as presenting strong antiadhesive effects in vitro and protect in vivo against colitis implying that they could be interesting compounds to treat AIEC-colonized CD patients [156]. Similar results were obtained with other analogues of HM and heptylmannoside grafted on cellulose nanofibers [157,158]. Targeting FimH with antiadhesive molecules is consequently a promising method to limit AIEC adhesion to IECs (Figure 3).…”

Section: Inhibition Of Aiec Adhesion Using Chemical Compoundsmentioning

confidence: 55%

Adherent-Invasive E. coli: Update on the Lifestyle of a Troublemaker in Crohn’s Disease

Chervy

Barnich

Denizot

2020

IJMS

Self Cite

View full text Add to dashboard Cite

Besides genetic polymorphisms and environmental factors, the intestinal microbiota is an important factor in the etiology of Crohn’s disease (CD). Among microbiota alterations, a particular pathotype of Escherichia coli involved in the pathogenesis of CD abnormally colonizes the intestinal mucosa of patients: the adherent-invasive Escherichia coli (AIEC) pathobiont bacteria, which have the abilities to adhere to and to invade intestinal epithelial cells (IECs), as well as to survive and replicate within macrophages. AIEC have been the subject of many studies in recent years to unveil some genes linked to AIEC virulence and to understand the impact of AIEC infection on the gut and consequently their involvement in CD. In this review, we describe the lifestyle of AIEC bacteria within the intestine, from the interaction with intestinal epithelial and immune cells with an emphasis on environmental and genetic factors favoring their implantation, to their lifestyle in the intestinal lumen. Finally, we discuss AIEC-targeting strategies such as the use of FimH antagonists, bacteriophages, or antibiotics, which could constitute therapeutic options to prevent and limit AIEC colonization in CD patients.

show abstract

“…over the 33 reactive groups present in 3) were conjugated to 3 by CuAAC, with tris(benzyltriazolylmethyl)amine (TBTA), L-sodium ascorbate and CuSO 4 in PBS similarly to previous reports. [26,27,28] After 24 h at room temperature, the resulting homovalent neoGPs 4 a-c were purified on Sephadex G25. The purity was confirmed by SDS-PAGE and MALDI-TOF experiments enabled to estimate that between 12 to 19 tetravalent glycodendrons were coupled to BSA, which corresponds to 49-75 glycans per BSA and molecular weights from 92 to 105 kDa for homovalent neoGPs 4 a-c (Table 1, FigureS11 and S14).…”

Section: Synthesis Of Neogpsmentioning

confidence: 99%

“…The binding ability of the homovalent neoGPs 4 a-c was studied with LecA and LecB using the GLYcoPROFILE® technology (GLYcoDiag, France). [26,27,28,31] NeoGPs 4 a-c were first labelled with biotin using the protocol described previously then were incubated in microtitration plates coated with lectins. Streptavidin labelled with 5-(4,6-dichlorotriazinyl)-aminofluorescein (DTAF) was finally incubated in each well and the interaction was directly detected by reading the fluorescence intensity (FI) provided by the neoGP bound to the lectin.…”

Section: Direct Binding Assaysmentioning

confidence: 99%

Homo‐ and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Goyard

Roubinet²,

Vena³

et al. 2021

ChemPlusChem

Self Cite

View full text Add to dashboard Cite

Click chemistry gives access to unlimited set of multivalent glycoconjugates to explore carbohydrate-protein interactions and discover high affinity ligands. In this study, we have created supramolecular systems based on a carrier protein that was grafted by Cu(I)-catalyzed azide-alkyne cycloaddition with tetravalent glycodendrons presenting αGal, βGal and/or αFuc. Binding studies of the homo-(4 a-c) and heterovalent ( 5) neoglycoproteins (neoGPs) with the LecA and LecB lectins from P. aeruginosa has first confirmed the interest of the multivalent presentation of glycodendrons by the carrier protein (IC 50 up to 2.8 nM). Moreover, these studies have shown that the heterovalent display of glycans (5) allows the interaction with both lectins (IC 50 of 10 nM) despite the presence of unspecific moieties, and even with similar efficiency for LecB. These results demonstrate the potential of multivalent and multispecific neoGPs as a promising strategy to fight against resistant pathogens.

show abstract

Heptylmannose-functionalized cellulose for the binding and specific detection of pathogenic E. coli

Abstract: We developed a chemical method to covalently functionalize cellulose nanofibers and cellulose paper with mannoside ligands displaying a strong affinity for the FimH adhesin from pathogenic E. coli strains.

Cited by 15 publications

References 25 publications

Heteropolysaccharides from S. cerevisiae show anti-adhesive properties against E. coli associated with Crohn's disease

Heteropolysaccharides from S. cerevisiae show anti-adhesive properties against E. coli associated with Crohn's disease

Adherent-Invasive E. coli: Update on the Lifestyle of a Troublemaker in Crohn’s Disease

Homo‐ and Heterovalent Neoglycoproteins as Ligands for Bacterial Lectins

Contact Info

Product

Resources

About