Hepcidin, an overview of biochemical and clinical properties

Rauf, Abdur; Shariati, Mohammad Ali; Khalil, Anees Ahmed; Bawazeer, Sami; Heydari, Mojtaba; Plygun, Sergey; Лаишевцев, А. И.; Hussain, Muhammad Bilal; Alhumaydhi, Fahad A.; Aljohani, Abdullah S. M.

doi:10.1016/j.steroids.2020.108661

Cited by 22 publications

(34 citation statements)

References 90 publications

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“…Naturally-occurring antimicrobial peptides (AMPs) represent a valid alternative to traditional antibiotics, as they are active against a broad spectrum of microbes, from Gram-positive and Gram-negative bacteria to fungi and viruses [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ]. Furthermore, compared to conventional drugs, they have further biological functions, such as a wound-healing, antidiabetic, anti-inflammatory, and immune-modulatory activities [ 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. Amphibian skin secretion is considered a rich source of broad-spectrum AMPs, and over the years, numerous peptides have been isolated and classified into the corresponding families, such as esculentins, temporins, and bombinins H [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Frog Skin-Derived Peptides Against Corynebacterium jeikeium: Correlation between Antibacterial and Cytotoxic Activities

et al. 2020

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Corynebacterium jeikeium is a commensal bacterium that colonizes human skin, and it is part of the normal bacterial flora. In non-risk subjects, it can be the cause of bad body smell due to the generation of volatile odorous metabolites, especially in the wet parts of the body that this bacterium often colonizes (i.e., groin and axillary regions). Importantly, in the last few decades, there have been increasing cases of serious infections provoked by this bacterium, especially in immunocompromised or hospitalized patients who have undergone installation of prostheses or catheters. The ease in developing resistance to commonly-used antibiotics (i.e., glycopeptides) has made the search for new antimicrobial compounds of clinical importance. Here, for the first time, we characterize the antimicrobial activity of some selected frog skin-derived antimicrobial peptides (AMPs) against C. jeikeium by determining their minimum inhibitory and bactericidal concentrations (MIC and MBC) by a microdilution method. The results highlight esculentin-1b(1-18) [Esc(1-18)] and esculentin-1a(1-21) [Esc(1-21)] as the most active AMPs with MIC and MBC of 4–8 and 0.125–0.25 µM, respectively, along with a non-toxic profile after a short- and long-term (40 min and 24 h) treatment of mammalian cells. Overall, these findings indicate the high potentiality of Esc(1-18) and Esc(1-21) as (i) alternative antimicrobials against C. jeikeium infections and/or as (ii) additives in cosmetic products (creams, deodorants) to reduce the production of bad body odor.

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Section: Introductionmentioning

confidence: 99%

Frog Skin-Derived Peptides Against Corynebacterium jeikeium: Correlation between Antibacterial and Cytotoxic Activities

et al. 2020

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“…Hepcidin is the main substance that regulates iron metabolism and acts on membrane iron transporter-1 on the reticuloendothelial system, duodenal epithelial cells, hepatocytes, and other cell membranes ( 28 ). Hepcidin can internalize and degrade iron, reduce the absorption of oral iron, and release stored iron.…”

Section: Discussionmentioning

confidence: 99%

“…Hepcidin can internalize and degrade iron, reduce the absorption of oral iron, and release stored iron. At last, hepcidin will induce an increase in intracellular iron storage and a decrease in circulating iron levels, which in turn leads to a decrease in the availability of iron in circulation ( 28 ). Some studies have shown that hepcidin and its receptors also exist in brain tissue and it can also participate in the regulation of iron metabolism in the brain ( 29 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, due to the increase of serum ferritin secondary to inflammation and intravenous or oral iron supplements in ESRD patients, the level of serum ferritin does not fully reflect decreased iron stores in these patients (7). Hepcidin is the main substance that regulates iron metabolism and acts on membrane iron transporter-1 on the reticuloendothelial system, duodenal epithelial cells, hepatocytes, and other cell membranes (28). Hepcidin can internalize and degrade iron, reduce the absorption of oral iron, and release stored iron.…”

Section: Discussionmentioning

confidence: 99%

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Relation of Serum Hepcidin Levels and Restless Legs Syndrome in Patients Undergoing Peritoneal Dialysis

et al. 2021

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Introduction: Restless legs syndrome is a common and severe complication in patients undergoing peritoneal dialysis (PD), which seriously affects the life quality and prognosis of patients undergoing PD. Unfortunately, there are still no effective prevention and treatment measures. Serum hepcidin was demonstrated to be related to primary restless legs syndrome (RLS), whereas there are no studies on the relationship between serum hepcidin and RLS in patients undergoing PD. We aimed to evaluate the role and function of serum hepcidin in patients undergoing PD with RLS.Methods: A total of 51 patients undergoing PD with RLS and 102 age-and gender-matched patients undergoing PD without RLS were included. We collected the clinical data including serum hepcidin of those patients undergoing PD. We scored the severity of RLS according to the International restless leg Syndrome Research Group rating scale (IRLS). We compared the clinical characteristics of the two groups and evaluated the determinant factors of RLS by Logistic regression analysis. In addition, we evaluated the diagnostic value of serum hepcidin in patients undergoing PD with RLS by receiver operating characteristic (ROC) curve. We also analyzed the influencing factors of IRLS by multivariate linear regression analysis.Results: The duration of PD, serum hepcidin, and calcium were found to be significantly higher in patients undergoing PD with RLS than those patients undergoing PD without RLS (P < 0.001, P < 0.001, and P = 0.002, respectively). The level of hemoglobin, albumin, and RKF were significantly lower in patients undergoing PD with RLS (P = 0.002, P = 0.042, and P < 0.001, respectively). The duration of PD [odds ratio (OR) 1.038, 95% CI: 1.017, 1.060, P < 0.001], hemoglobulin level (OR 0.969, 95% CI: 0.944, 0.995, P = 0.019), calcium level (OR 9.224, 95% CI: 1.261, 67.450, P = 0.029), albumin level (OR 0.835, 95% CI: 0.757, 0.921, P < 0.001), hepcidin level (OR 1.023, 95% CI: 1.009, 1.038, P = 0.001), and RKF (OR 0.65, 95% CI: 0.495, 0.856, P = 0.002) are independent determinant factors of RLS in patients undergoing PD. Multivariate linear regression analysis revealed that, in addition to albumin, they were also independently associated with the severity of RLS.Conclusion: A significant relation was detected between serum hepcidin level and RLS in patients undergoing PD.

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“…Alternatively, depending on their final effect, AMPs can be separated into membrane disruptive AMPs and non-membrane disruptive AMPs [31]. In addition, during infection, hepcidin [32] reveals a defensive role through iron concentrations depletion while histatin shows a cytotoxic action similar to the candidacidal activity of human neutrophil defensin 1 (HNP-1) [23,33].…”

Section: Renewing Antimicrobial Peptides Potentialmentioning

confidence: 99%

Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

Marrazzo

Pizzuti

Zia³

et al. 2021

Antibiotics

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Antibiotic resistance is creating enormous attention on the development of new antibiotic-free therapy strategies for bacterial diseases. Mesenchymal stromal stem cells (MSCs) are the most promising candidates in current clinical trials and included in several cell-therapy protocols. Together with the well-known immunomodulatory and regenerative potential of the MSC secretome, these cells have shown direct and indirect anti-bacterial effects. However, the low reproducibility and standardization of MSCs from different sources are the current limitations prior to the purification of cell-free secreted antimicrobial peptides and exosomes. In order to improve MSC characterization, novel label-free functional tests, evaluating the biophysical properties of the cells, will be advantageous for their cell profiling, population sorting, and quality control. We discuss the potential of emerging microfluidic technologies providing new insights into density, shape, and size of live cells, starting from heterogeneous or 3D cultured samples. The prospective application of these technologies to studying MSC populations may contribute to developing new biopharmaceutical strategies with a view to naturally overcoming bacterial defense mechanisms.

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Hepcidin, an overview of biochemical and clinical properties

Cited by 22 publications

References 90 publications

Frog Skin-Derived Peptides Against Corynebacterium jeikeium: Correlation between Antibacterial and Cytotoxic Activities

Frog Skin-Derived Peptides Against Corynebacterium jeikeium: Correlation between Antibacterial and Cytotoxic Activities

Relation of Serum Hepcidin Levels and Restless Legs Syndrome in Patients Undergoing Peritoneal Dialysis

Microfluidic Tools for Enhanced Characterization of Therapeutic Stem Cells and Prediction of Their Potential Antimicrobial Secretome

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