Hepatotropic Effects of Triple Antiulcer Therapy and Placenta Cryoextract: The Role of Sex Factors in Lipoperoxidation

Koshurba, I.V.; Hladkykh, Fedir; Chyzh, Mykola; Belochkina, Iryna; Rubleva, T.V.

doi:10.15407/fz68.05.025

Cited by 12 publications

(8 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Considering gender differences in drug metabolism and the physiological characteristics of the GIT between men and women, the optimisation of treatment regimens for the most prevalent digestive system disorders, taking into account sex hormone levels, becomes a pivotal goal in contemporary gastroenterology and hepatology. 3,4 Of particular interest is the gender-based differentiation of treatment approaches in patients with comorbid conditions, due to the risk of interactions between drugs and the influence of concomitant diseases on clinical outcomes. In patients with liver diseases, the most common comorbid conditions among others include arterial hypertension, oesophagitis, dyslipidaemia, diabetes mellitus and peptic ulcer disease (PUD).…”

Section: Discussionmentioning

confidence: 99%

“…Given the central role of hepatic enzymes in regulating the pharmacological and biological activity of drugs, as well as steroids and other endobiotics, it is imperative to comprehend the regulatory characteristics that contribute to individual disparities in their expression. 3 It is noteworthy that the most crucial isoform of CYP in drug metabolism, CYP 3A4, exhibits higher expression levels in the liver of women as compared with men. 4, 5…”

Section: Biologically Active Substancesmentioning

confidence: 99%

See 1 more Smart Citation

Sex differences in the hepatotropic effects of antiulcer drugs and placenta cryoextract in an experimental rat liver injury model

Hladkykh,

Koshurba,

Komorovsky

et al. 2023

Scripta Medica

View full text Add to dashboard Cite

Background/Aim: Sex-related variances in drug metabolism provide a foundation for refining treatment protocols for prevalent conditions based on the patient's sex. Tailoring treatment strategies based on sex is particularly noteworthy among patients with comorbid illnesses due to the potential for drug interactions and the impact of concurrent diseases on clinical outcomes. Aim of this study was to assess the hepatotropic effects of antiulcer drugs (esomeprazole, clarithromycin and metronidazole - E/C/M) and placenta cryoextract (CEP) within a simulated model of tetrachloromethane (CCl4 )-induced hepatitis combined with underlying ethanol-induced liver cirrhosis (EILC), with a focus on the role of subjects' sex. Methods: Using 112 male and female rats, the research explored the effects of different sex hormone levels. Chronic EILC was induced by administering a 50.0 % CCl4 oil solution (8 mL/kg) twice a week, combined with a 5.0 % ethanol solution, over 45 days. Total protein (TP) levels and alkaline phosphatase (AP) activity were measured spectrophotometrically. Results: The research findings indicate that the onset of EILC and the administration of E/C/M resulted in a significantly greater 10.8 % (p = 0.03) reduction in TP levels among females compared to males, without altering hormonal status. Introducing CEP led to a noteworthy (p < 0.001) rise in TP levels, by 30.8 % in males and 33.9 % in females, in the context of EILC and E/C/M administration, while maintaining hormonal status. Among male rats, the most elevated AP activity was observed with excess testosterone propionate administration (5.0 [5.0; 5.9] mmol/L), while the lowest level was recorded in rats after testectomy, measuring 3.8 [2.5; 4.7] mmol/L, exhibiting a significant 20.8 % decrease (p < 0.05) compared to male rats without hormonal status changes. In female rats, the study revealed that against the backdrop of EILC and E/C/M administration, the highest AP level was seen in ovariectomised females, reaching 5.8 [5.1; 6.2] mmol/L, reflecting a substantial 9.4 % increase compared to rats without hormonal status changes. Conclusions: The administration of CEP under similar experimental conditions led to the recovery of the liver's protein-synthesising function in both male and female rats. When female sex hormones were introduced to sham-operated female rats, a significant 20.8 % greater reduction in AP levels was observed. Additionally, gonadectomy led to a more pronounced decrease in this enzyme's levels in male rats compared to female rats, indicating the cytoprotective properties of female sex hormones.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Biologically Active Substancesmentioning

confidence: 99%

Sex differences in the hepatotropic effects of antiulcer drugs and placenta cryoextract in an experimental rat liver injury model

Hladkykh,

Koshurba,

Komorovsky

et al. 2023

Scripta Medica

View full text Add to dashboard Cite

show abstract

“…Вибір КЕП як засобу для стимуляції нейрорегенерації після СКШ обумовлений наявністю у нього, крім нейротропної дії, цілої низки цінних біологічних ефектівпротизапальної, антиоксидантної, імуномоделювальної, репаративної, гепатопротекторної, нефропротекторної, метаболотропної, остеотропної, кардіопротекторної дії тощо [28][29][30][31]. Плацентарна терапія застосовується в комплексному лікуванні захворювань і регенерації тканин від початку XX ст.…”

Section: вступunclassified

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Кошурба

Hladkykh

Chyzh

et al. 2023

Innov Biosyst Bioeng

View full text Add to dashboard Cite

Background. Peptic ulcer is a prevalent gastrointestinal tract disease worldwide. One of the treatment methods for the indicated pathology is trunk gastric cryodenervation (TGC), the feature of which is the preservation of the regenerative potential of n. vagus. Cryopreserved placenta extract (CPE) caught our attention among modern means capable of stimulating regeneration processes. Objective. We are aimed to characterize the state of gastric secretion after TGC and the application of СPE. Methods. TGC was performed by cryoinfusion on the front and back trunks of the abdominal part of n. vagus. Gastric secretion was studied using the H.A. Shay method. Results. On the 30th day after TGC, a statistically significant (p < 0.001) decrease in the total acidity of gastric juice by 33.7% and a decrease (p < 0.01) in the level of free acidity were observed compared to intact animals. Administration of СPE after TGC resulted in a statistically significant (p < 0.001) decrease in total acidity by 24.2 % and a statistically significant (p < 0.001) decrease in free acidity by 48.7% compared to the parameters of intact rats. This led to a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals (1.4 ± 0.03 RU) which was higher than the similar ratio observed after esomeprazole usage or TGC. Conclusions. Administration of СPE contributed to the partial regeneration of n. vagus, as indicated by an increase in the level of total acidity to 104.1 ± 4.7 RU and a statistically significant (p < 0.001) decrease in the "free/bound acidity" ratio compared to the indicator of intact animals.

show abstract

“…The antioxidant-prooxidant index (API) was calculated using the formula: API = (Catalase Activity 100) / Thiobarbituric Acid Reactive Substances (TBA-RP) content [26]. 22,2022).…”

Section: Modeling Of Experimental Pathology Chronic Ccl4-induced Hepa...mentioning

confidence: 99%

Gender Differences in the Effect of Antiulcer Drugs and Placenta Cryoextract on the Intensity of Lipid Peroxidation and the Activity of the Antioxidant System in Experimental Hepatitis With Ethanol-Induced Cirrhosis

Koshurba,

Hladkykh,

Chyzh

et al. 2023

Clin. and prev. med.

View full text Add to dashboard Cite

Introduction. It is well recognized that drug metabolism products in the liver can induce oxidative stress and mitochondrial dysfunction, leading to the development of hepatocellular injury. As a potential agent capable of counteracting the hepatotoxic effects of drugs, we focused our attention on a domestic biotechnological preparation – cryopreserved placental extract (CPE). The aim. To characterize gender differences in the effect of esomeprazole, clarithromycin, metronidazole (E/C/M), and CPE on the intensity of lipid peroxidation and the activity of the antioxidant system in tetrachloromethane (CCl4) hepatitis with a background of ethanol-induced cirrhosis (ETCM). Materials and methods. The study was conducted with varying levels of sex hormones on 112 male and female rats. Chronic ETCM was induced by administering a 50.0% oil solution of CCl4 at a dose of 8 ml/kg body weight of the animals twice a week, in combination with a 5.0% ethanol solution for drinking over a period of 45 days. The content of TBA-RP in liver homogenates was determined spectrophotometrically by the method described by Asakawa T. et al. Catalase activity in liver homogenates was determined spectrophotometrically according to the method of Korolyuk M.A. and co-authors. Results. The most pronounced increase in lipid peroxidation processes was observed in females with chronic ETCM-induced liver damage and administration of antiulcer drugs following ovariectomy, resulting in a TBA-RS content of 36.1±2.79 μmol/kg of tissue. Administration of E/C/M in animals with chronic liver damage led to a suppression of the antioxidant system, as evidenced by a decrease in catalase activity in liver tissues. Conclusion. The combined use of anti-ulcer drugs and CPE on the background of chronic ETCM mitigated the activation of lipid peroxidation processes, which was indicated by a statistically significant (p < 0.001) 2.7-fold lower content of TBA-RP in liver homogenates. Additionally, it was established that the administration of CPE was accompanied by a statistically significant increase in catalase activity in females, more prominently than in males. In females without changes in hormonal status, the introduction of CPE resulted in a growth (p < 0.001) of catalase activity by 75.0%, with the most significant increase observed in females after ovariectomy – catalase activity statistically significantly (p < 0.001) increased by 2.6 times compared to the indicators of females not administered with CPE. The administration of CPE in female rats without altering hormonal status was accompanied by a twofold (p < 0.01) increase in the antioxidant-prooxidant index compared to male rats, indicating more pronounced antioxidant properties of CPE in female rats.

show abstract

Hepatotropic Effects of Triple Antiulcer Therapy and Placenta Cryoextract: The Role of Sex Factors in Lipoperoxidation

Cited by 12 publications

References 17 publications

Sex differences in the hepatotropic effects of antiulcer drugs and placenta cryoextract in an experimental rat liver injury model

Sex differences in the hepatotropic effects of antiulcer drugs and placenta cryoextract in an experimental rat liver injury model

The Characteristic of the Gastric Secretion After Gastric Cryodenervation and Cryopreserved Placenta Extract Administration

Gender Differences in the Effect of Antiulcer Drugs and Placenta Cryoextract on the Intensity of Lipid Peroxidation and the Activity of the Antioxidant System in Experimental Hepatitis With Ethanol-Induced Cirrhosis

Contact Info

Product

Resources

About