Hepatotoxicity; Protective Effect of Silymarin Against Methotrexate Induced in Mice

Abstract

Thirty male BALB/c mice were randomly divided into five groups (n=6). Group A received 0.2 ml normal saline intraperitoneally served as control for MTX. Group B received 0.2 ml distilled water orally for 7 days served as control for oral silymarin. Group C received single intraperitoneal injection of MTX 20 mg/kg. Group D received silymarin 25 mg/kg orally for seven days. Group E received silymarin 25 mg/kg orally for 7 days with MTX 20 mg/kg intraperitoneally at day 4. Blood samples for measuring serum ALT (Alanine Transaminase), AST (Aspartate transaminase) and ALP (Alkaline Phosphatase) along with liver samples for hepatic histological examination were taken after 24 hours of last dose. Results: Silymarin show hepatoprotective effect against MTX induced hepatotoxicity. Conclusion: Silymarin has hepatoprotective potential when administered along with MTX. Key words:Hepatoprotective, Hepatotoxicity, Methotrexate, Silymarin.

The Effect of Pretreatment With Toll-Like Receptor 4 Antagonist Resatorvid on Methotrexate-Induced Liver Injury in Rats: Histopathological Study

“…Moderate inflammation observed in the histological section of treated animals with infiltration of chronic inflammatory cells. The histopathological changes we achieved in this study are in consistent with the previous studies, which described severe focal necrosis accompanied by hepatic granular damage, abnormal hepatocytes rearrangement around central vein with shrunken and inflected nuclei, infiltration of mononuclear cells, vascular congestion, increased KCs activation and proliferated connective tissue in hepatic sections of those rats treated with MTX [15,20,21,34,[41][42][43]. Despite, our finding represent the moderate-severe patterns of MTX-induced liver injury and thus we described milder steatohepatitic changes [11,31,34,39,40].…”