Hepatotoxic Effect of Oral Zinc Oxide Nanoparticles and the Ameliorating Role of Selenium in Rats: A histological, immunohistochemical and molecular study

Aboulhoda, Basma Emad; Abdeltawab, Dina Adel; Rashed, Laila Ahmed; Alla, Marwa Fathi Abd; Yassa, Hanan Dawood

doi:10.1016/j.tice.2020.101441

Cited by 20 publications

(8 citation statements)

References 55 publications

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“…MDA, GSH, SOD, and CAT are indicators for oxidative stress. Our results coincided with Aboulhoda et al [ 50 ] who reported decreased antioxidative enzyme activity and increased MDA with oral ZON. The mechanism of ZON tissue injury may be by increased ROS generation when ZON penetrate into the cytoplasm of mammalian cells.…”

Section: Discussionsupporting

confidence: 92%

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Badran,

El-Agroudy,

Kassab

et al. 2024

Anat Cell Biol

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The world has witnessed tremendous advancements in nano-base applications. Zinc oxide nanoparticles (ZON) are widely used in food industry and medicine. Although their application is of important value, they may cause toxicity to body tissues. Peripheral blood mononuclear cells (PBMCs) proved its efficacy in tissue regeneration especially when it is preconditioned by activated platelet supernatant (APS). The aim of this study is to evaluate the effect of ZON on the gastric mucosa and the therapeutic role of the PBMCs preconditioned by APS in rats. Ten rats were donors and fifty rats were recipients. The recipients were divided into; control group, ZON group (10 mg/kg/day orally for five days) and preconditioned PBMCs group (1×10 7 once intravenously 24 hours after ZON). Gastric specimens were processed for histological, immunohistochemical, biochemical and quantitative real-time polymerase chain reaction studies. ZON group showed marked structural changes in the gastric mucosa. There was desquamation or deep ulceration of the epithelium. Cytoplasmic vacuoles and pyknotic nuclei were in glandular cells. Reduced proliferating cell nuclear antigen and increased tumor necrosis factor-α were in epithelial cells. There were significant elevation in malondialdahyde and reduction in glutathione, superoxide dismutase, and catalase. Enhancement in mRNA expression of nuclear factor kappa-B and cyclooxygenase-2 was detected. The preconditioned PBMCs group showed significant improvement of all parameters. So, ZON had cytotoxic effects on the gastric mucosa and the preconditioned PBMCs had a therapeutic effect on gastric mucosal damage after ZON.

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Section: Discussionsupporting

confidence: 92%

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Badran,

El-Agroudy,

Kassab

et al. 2024

Anat Cell Biol

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“…This parameter returned to normal when a later period was evaluated (20 days). In this case, different types of ZnO and Ag NPs have already been reported to induce hepatic and renal morphological changes, in addition to altering the same biochemical parameters observed in our study [ 35 , 36 , 37 , 38 , 39 ]. However, the changes reported here are less intense than those reported in the literature.…”

Section: Discussionsupporting

confidence: 77%

Toxicity Assessment of New Ag-ZnO/AgO Nanocomposites: An In Vitro and In Vivo Approach

do Carmo Neto,

Franco,

Braga

et al. 2024

JFB

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Zinc oxide nanoparticles (ZnO NPs) are metal oxide nanomaterials, which are important for several applications: antibacterial, anthelmintic, antiprotozoal and antitumoral, among others. These applications are mainly related to the ability to spontaneously produce and induce the production of reactive oxygen species that are important components for the destruction of pathogens and tumor cells. While trying to potentiate ZnO NPs, studies have associated these NPs with silver oxide (AgO) or silver (Ag) NPs. It has already been reported that this combination (Ag-ZnO/AgO NPs) is able to enhance the microbicidal potential. Although possessing much potential for several purposes, it is important to evaluate whether this association also poses the risk of toxicity to cells and experimental models. Therefore, this work aimed to evaluate the toxicity of various Ag-ZnO/AgO NP nanocomposites, in vitro and in vivo. Accordingly, ZnO nanocrystals and nanocomposites with various concentrations of AgO (ZnO:5Ag, ZnO:9Ag or ZnO:11Ag) were used in different cytotoxicity models: Galleria mellonella (G. mellonella), cell lines (VERO and RAW 264.7) and C57BL/6 mice. In the G. mellonella model, four concentrations were used in a single dose, with subsequent evaluation of mortality. In the case of cells, serial concentrations starting at 125 µg/mL were used, with subsequent cytotoxicity assessment. Based on the safe doses obtained in G. mellonella and cell models, the best doses were used in mice, with subsequent evaluations of weight, biochemistry as also renal and liver histopathology. It was observed that the toxicity, although low, of the nanocomposites was dependent upon the concentration of AgO used in association with ZnO NPs, both in vitro and in vivo.

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“…Therefore, the in vivo effects of high doses of nano-TiO 2 on liver function have been extensively studied, while similar information on the study of the effect of ZnO NPs administered orally is much more limited, but the available data indicate the cytotoxic (in particular, hepatotoxic) effect of this nanomaterial, the main mechanism of which is the activation of oxidative stress in cells. − Thus, the results of studies by Aboulhoda et al, in which ZnO NPs (size about 7 nm, doses used 100–300 mg/kg per day) were orally applied to Sprague–Dawley rats for 14 days, showed the activation of oxidative stress, apoptosis, and hepatotoxicity of nano-ZnO. Using female mice, Esmaeillou et al showed that oral intake of nano-ZnO (dimensions about 20–30 nm, used doses are 333.33 mg/kg per day) is accompanied by significant structural and functional disorders of the tissues of the liver, kidneys, lungs, and genitals; in particular, animals were characterized by a significant (by a quarter of the control) decrease in the thickness of the myometrium and had a significant increase in the activities of ALT and AST in the blood plasma.…”

Section: Resultsmentioning

confidence: 99%

ZnO and TiO₂ Nanocolloids: State of Mechanisms that Regulating the Motility of the Gastrointestinal Tract and the Hepatobiliary System

et al. 2021

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Using the transmission electron microscopy (TEM)/high-resolution TEM (HRTEM) and selected area electron diffraction (SAED) methods, it was shown that the nanocolloids of ZnO contain hydrolyzed ZnO nanoparticles (NPs). Typically, the nanocrystalline ZnO/Zn(OH)2 core is covered by an amorphous shell of zinc hydroxides, preventing the encapsulated crystal core from dissolving. Similar studies were carried out with TiO2 nanocolloids. It was found that burdening of rats for 30 days with a ZnO aqueous nanocolloid (AN) was accompanied by a narrowing of the amplitude range, a decrease (increase) in the frequency of spontaneous contractions (SCs), and an inhibition of the efficiency indices for smooth muscles (SMs) of the antrum and cecum. Under longer (100 days) burdening of rats with AN of ZnO, there was a tendency toward restoring the above parameters. In terms of the value and the direction of changes in most parameters for SCs of SMs, the effects (30 days) of TiO2 AN differed from those for ZnO AN and were almost the same in the case of their long-term impact. It was found that mostly M2-cholinoreceptor-dependent mechanisms of regulating the intracellular concentration of Ca2+ were sensitive to the effect of ZnO and TiO2 ANs. The molecular docking demonstrated that ZnO and TiO2 NPs did not compete with acetylcholine for the site of binding to M3 and M2 cholinoreceptors but may impact the affinity of orthosteric ligands to M2 cholinoreceptors. The studies showed that burdening rats with ZnO and TiO2 ANs was also accompanied by changes in the activity state of both intracellular enzymes and the ion transport systems for Na+, K+, and Ca2+, related to the processes of bile secretion, via the plasma membrane of hepatocytes.

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Hepatotoxic Effect of Oral Zinc Oxide Nanoparticles and the Ameliorating Role of Selenium in Rats: A histological, immunohistochemical and molecular study

Cited by 20 publications

References 55 publications

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Toxicity Assessment of New Ag-ZnO/AgO Nanocomposites: An In Vitro and In Vivo Approach

ZnO and TiO₂ Nanocolloids: State of Mechanisms that Regulating the Motility of the Gastrointestinal Tract and the Hepatobiliary System

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Hepatotoxic Effect of Oral Zinc Oxide Nanoparticles and the Ameliorating Role of Selenium in Rats: A histological, immunohistochemical and molecular study

Cited by 20 publications

References 55 publications

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Impact of peripheral blood mononuclear cells preconditioned by activated platelet supernatant in managing gastric mucosal damage induced by zinc oxide nanoparticles in rats

Toxicity Assessment of New Ag-ZnO/AgO Nanocomposites: An In Vitro and In Vivo Approach

ZnO and TiO2 Nanocolloids: State of Mechanisms that Regulating the Motility of the Gastrointestinal Tract and the Hepatobiliary System

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Product

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ZnO and TiO₂ Nanocolloids: State of Mechanisms that Regulating the Motility of the Gastrointestinal Tract and the Hepatobiliary System