Hepatoprotective treatment attenuates oxidative damages induced by carbon tetrachloride in rats

Belo, Marco Antônio de Andrade; Soares, Vando Edésio; Souza, Luciano Melo de; Sobreira, Márcia Ferreira da Rosa; Cassol, Daniela; Toma, Sandra Barioni

doi:10.1016/j.etp.2010.08.001

Cited by 18 publications

(10 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This decrease in enzyme activity was attributed to the increase in free radicals that affected the inner mitochondrial membrane and intracellular calcium stores led to structural and functional disorganization, over all loss in energy production, irreversible damage, and loss of enzymatic activity [55,56]. Rusu et al [54] and De-Andrade Belo et al [57] confirmed the decrease in hepatic LDH after CCl 4 intoxication. Enzyme inhibition was mainly due to increase membrane fluidity as a result of ROS involvement, leads to enzyme leakage into circulation [50].…”

Section: Discussionmentioning

confidence: 99%

Zingiber officinale acts as a nutraceutical agent against liver fibrosis

Motawi

Hamed

Shabana³

et al. 2011

Nutr Metab (Lond)

View full text Add to dashboard Cite

Background/objectiveZingiber officinale Roscoe (ginger) (Zingiberaceae) has been cultivated for thousands of years both as a spice and for medicinal purposes. Ginger rhizomes successive extracts (petroleum ether, chloroform and ethanol) were examined against liver fibrosis induced by carbon tetrachloride in rats.ResultsThe evaluation was done through measuring antioxidant parameters; glutathione (GSH), total superoxide dismutase (SOD) and malondialdehyde (MDA). Liver marker enzymes; succinate and lactate dehydrogenases (SDH and LDH), glucose-6-phosphatase (G-6-Pase), acid phosphatase (AP), 5'- nucleotidase (5'NT) and liver function enzymes; aspartate and alanine aminotransferases (AST and ALT) as well as cholestatic markers; alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total bilirubin were estimated. Liver histopathological analysis and collagen content were also evaluated. Treatments with the selected extracts significantly increased GSH, SOD, SDH, LDH, G-6-Pase, AP and 5'NT. However, MDA, AST, ALT ALP, GGT and total bilirubin were significantly decreased.ConclusionsExtracts of ginger, particularly the ethanol one resulted in an attractive candidate for the treatment of liver fibrosis induced by CCl4. Further studies are required in order to identify the molecules responsible of the pharmacological activity.

show abstract

Section: Discussionmentioning

confidence: 99%

Zingiber officinale acts as a nutraceutical agent against liver fibrosis

Motawi

Hamed

Shabana³

et al. 2011

Nutr Metab (Lond)

View full text Add to dashboard Cite

show abstract

“…Tais achados corroboram estudos realizados com roedores após administração de CCl 4 nos quais se observou mudanças hepáticas como hepatomegalia, esteatose, inflamação, infiltração celular, dilatação dos sinusóides e da veia centrolobular (Goessling & Sadler 2015). Estas alterações hepáticas na fase mais aguda da intoxicação por este composto orgânico resultaram em aumento significativo do índice somático hepático em ratos Wistar (Belo et al 2012b). De acordo com Lawrence & Steiner (2017), o aumento da atividade de AST no soro é considerado um marcador sensível para a lesão hepatocelular principalmente quando associado a outros indicadores de lesões hepáticas como a esteatose.…”

Section: Resultsunclassified

“…Indução da esteatose hepática. Para indução da esteatose hepática, utilizou-se CCl 4 (Merck  ) em dose única de 0,5mL/kg, homogeneizado com azeite de oliva na proporção de 1:1, v:v, administrado por via intraperitoneal adaptado de Belo et al (2012b). No grupo sem tratamento com CCl 4 houve à aplicação somente do azeite de oliva em mesmo volume e local para padronização do estímulo de manipulação.…”

Section: Methodsunclassified

Esteatose hepática altera acúmulo celular em tilápias do Nilo durante aerocistite infecciosa

Moraes

Prado

Foz³

et al. 2018

Pesq. Vet. Bras.

View full text Add to dashboard Cite

RESUMO: O presente estudo avaliou a hepatotoxicidade induzida pelo CCl4 durante o efeito glicocorticoide da dexametasona (DEX) na fisiopatologia da reação inflamatória aguda em tilápias do Nilo, Oreochromis niloticus, correlacionando a funcionalidade hepática à cinética de acúmulo celular em aerocistite infecciosa. Para tal, utilizou-se 84 tilápias do Nilo distribuídas em 4 tratamentos: controle, CCl4, DEX e CCl4+DEX. Sendo amostrados 7 animais por tratamento em três períodos, isto é: seis, 24 e 48h após indução de inflamação. Utilizou-se CCl4 em dose única de 0,5mL/kg, via intraperitoneal para causar o transtorno hepático. Para indução da aerocistite utilizou-se inóculo de Aeromonas hydrophila. A dexametasona foi administrada via intramuscular na dose de 2 mg/kg de peso vivo. Os resultados revelaram que quanto maior foi à atividade sérica de aspartato aminotransferase (AST) maior foi a alteração somática do fígado, sendo estes achados inversamente proporcionais ao acúmulo celular no foco inflamatório, demonstrando menor número de células inflamatórias nos animais acometidos com maior grau de distúrbios hepáticos induzidos pelo CCl4. O estudo histopatológico revelou alterações degenerativas transitórias na fase mais aguda, pois os fígados das tilápias revelaram o acúmulo lipídeos nos hepatócitos 6h após administração de CCl4, sendo esta degeneração gordurosa não mais observada nos tempos de 24 e 48h. Contudo, a administração de CCl4 em tilápias do Nilo resultou em degeneração hepática aguda e transitória, caracterizada pelo acúmulo de gordura nos hepatócitos, aumento de AST no sangue e hepatomegalia. Com a disfunção hepática houve comprometimento do recrutamento celular em aerocistite infecciosa, indicando que há participação do fígado na resposta imune inata em peixes.

show abstract

“…In the pathophysiology of these events, hepatocytes play a central role in the homeostatic balance of essential biological processes, regulating important metabolic functions involving the synthesis, activation, storage and catabolism of endogenous and exogenous chemicals [8]. According to Rivera-Huizar et al [9] and Belo et al [5], the occurrence of development of hepato-renal syndrome is high in cases of poisoning. Therefore severe damages to liver by different etiologies can impair the renal function and aggravate the clinical status of the animal.…”

Section: Introductionmentioning

confidence: 99%

“…According to Castro et al [3], all substance is potentially toxic, the correct dose differentiates a remedy of a toxicant. The practice of amino acids parenteral administration presents toxicological risks as observed with most chemical compounds such as spoiled food, industrial residues, among others, which induce lipid peroxidation mediated by the presence of free radicals, resulting in damage to biomembranes, consequently, promoting cellular and tissue dysfunctions [4,5].…”

Section: Introductionmentioning

confidence: 99%

Clinical safety of amino acids and vitamins administered through parenteral route

Moraes¹,

Scherma²,

Prado³

et al. 2017

Int J Vet Sci Res

View full text Add to dashboard Cite

The parenteral use of amino acids and vitamins has been used to improve performance in animal production. However, few studies demonstrating the toxicity of these compounds. The present investigation evaluated the toxicological and clinical safety of the injectable supplement compound by amino acids and vitamins, administered by intramuscular route in Wistar rats. 56 females (± 220g), pubescent virgins, were randomly divided into seven groups (n=8): Treated with dose of 0.2, 2 and 4 mL, sampled 24 and 96 hours post-treatment (HPT), totaling six groups and one control group (Physiology Standard). Signifi cant increase in the enzymatic activity of serum ALT, AST, LDH and ALP was observed in animals treated with 2 and 4 mL (24 HPT). However, these changes returned to baseline levels 96 HPT, except in serum AST and ALP in animals treated with 4 mL. CK-NAC showed dose-response effect, since the higher compound dose was followed by proportional increase in the circulating levels of this enzyme, associated to muscle degeneration and necrosis area in rats treat with 4mL at 96 HPT. The evaluation of hepatic function did not result in signifi cant changes in the serum levels of triglycerides, cholesterol, albumin, urea and creatinine in the comparison between rats treated with 0.2 mL and control rats, demonstrating the innocuousness of this treatment on the activity of liver and kidney. However, deleterious effects were observed in animals subjected to doses of 2 and 4 mL, including observed increased splenic and hepatosomatic response, signifi cant decrease in circulating albumin levels and signifi cant leukocytosis, which is characterized by neutrophilia, followed by lymphopenia demonstrating the compound irritating effect on muscle tissue.

show abstract

Hepatoprotective treatment attenuates oxidative damages induced by carbon tetrachloride in rats

Cited by 18 publications

References 44 publications

Zingiber officinale acts as a nutraceutical agent against liver fibrosis

Zingiber officinale acts as a nutraceutical agent against liver fibrosis

Esteatose hepática altera acúmulo celular em tilápias do Nilo durante aerocistite infecciosa

Clinical safety of amino acids and vitamins administered through parenteral route

Contact Info

Product

Resources

About