Hepatoprotective mechanisms of Ageratum conyzoides L. on oxidative damage induced by acetaminophen in Wistar rats

Abstract

Background and objectives: Drug induced hepatotoxicity is a major health issue concern by drug regulatory agencies, pharmaceutical industry and health care professionals. Present study was aimed to investigate the hepatoprotective mechanisms of n-hexane and acetone extract of Ageratum conyzoides on oxidative damage induced by acetaminophen (APAP) in Wistar rats. Materials and methods: 42 Wistar rats were randomly divided into seven groups. Group I and II receive distilled water, CMC respectively. Group III was fed with standard drug silymarin, group IV and V received plant extracts only whereas group VI and VII received pretreatment with acetone and n-hexane extracts for seven days respectively and APAP was administered on the 5th day. Samples were collected on 48 h of post APAP administration and analysis was done using standard protocols. Results: Significant (p < 0.05) increased in MDA, G6PD and GST and significant (p < 0.05) decrease in SOD, GSH, total thiols in liver tissue was observed in APAP exposed group as compared to control. The preexposure of acetone and n-hexane extracts of A. conyzoides fallowed by APAP exposure significantly (p < 0.05) reduce activities of G6PD, GST and MDA levels as compare to APAP exposed group, whereas total thiols and GSH levels are restored only in n-hexane extract of A. conyzoides. Conclusion: Observations of present study suggested that pre-exposure of n-hexane extract of A. conyzoides restore the levels of total thiols, GSH and GST activity which may be responsible for reducing oxidative damage induced by APAP administration.

Antioxidant and hepatoprotective effects of ethanolic and ethyl acetate stem bark extracts of Copaifera multijuga (Fabaceae) in mice

“…Many studies suggest that PCM is hepatotoxic when administered at high doses (Hinson et al 2010;Hodgman et al 2012;Kisaoglu et al 2014) and some liver enzymes were analysed in order to confirm this damage. The hepatotoxic damage of PCM was confirmed by the increase of ALT and AST in plasma, which was also observed in other studies using PCM in the same dose (250 mg kg -1 ) (Olaleye and Rocha 2008) and higher doses [Rosa et al 2012 (600 mg kg -1 ); Shanmugam et al 2013 (800 mg kg -1 ); Kisaoglu et al 2014 (1 g kg -1 ); Olaleye et al 2014 (2 g kg -1 ); and Verma et al 2013 (3 g kg -1 )]. EE, EA and rutin were able to reverse the damage caused by PCM on ALT, an enzyme found in the cytoplasm of hepatocytes (Nelson and Cox 2011), suggesting that they may act as hepatoprotectives.…”

Candesartan modulates the antioxidant effect of silymarin against CCl4-induced liver injury in rats

“…20 In the present study, the hepatic content of GSH as well as SOD activity were found to be decreased significantly in CCL 4 -intoxicated rats as compared with control rats.…”

Comparative in vitro studies of antiglycemic potentials and molecular docking of Ageratum conyzoides L. and Phyllanthus amarus L. methanolic extracts

“…The WHO Expert Committee on diabetes recommended that medicinal plants should be investigated further [28]. A. conyzoides, a family of asteraceae with an annual weed of 80-90 cm in height has been reported to exhibit antioxidant property [30]. Also, the P. amarus of the family Euphorbiaceae contains compounds like alkaloids, flavonoids, lignans, phenols and terpenes which have been shown to interact with most key enzymes such as amylase, glucosidase [31].…”