Hepatoprotective mechanism of schisandrin B: role of mitochondrial glutathione antioxidant status and heat shock proteins

Chiu, Po Yee; Tang, Man Ho; Mak, Duncan H.; Poon, Michel Kwong Tat; Ko, Kam Ming

doi:10.1016/s0891-5849(03)00274-0

Cited by 73 publications

(48 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Measurement of Prooxidant-Induced ROS Generation in AML12 Cells AML12 cells (1×10 5 ) were seeded on a 96-well black multi-titer plate with clear bottom, and they were allowed to grow overnight. The cells were washed twice with phosphate-buffered saline (PBS) and then incubated with 0.1% bovine serum albumin (BSA) in PBS containing 5 μM (final concentration) DCFDA (dissolved in 0.2% (v/v) ethanol) for 30 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…1) Our laboratory has shown that the induction of glutathione antioxidant response is essential to confer cellular/tissue protection against various oxidant-induced injuries. [2][3][4][5][6] Daily consumption of antioxidants, such as ascorbic acid and α-tocopherol, has been recommended for enhancing cellular antioxidant defense capacity. However, results obtained from epidemiological studies as well as clinical trials have failed to demonstrate the beneficial effects of antioxidant intake.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Prooxidant-Induced Glutathione Antioxidant Response in Vitro and in Vivo: A Comparative Study between Schisandrin B and Curcumin

Leong

Chiu

2012

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

We investigated whether two naturally-occurring prooxidants, namely, schisandrin B (Sch B) and curcumin, and a synthetic prooxidant, menadione, can invariably elicit cyto/hepatoprotective responses against oxidant-induced injury. Results showed that ( )Sch B (a potent enantiomer of Sch B, 15 μM), curcumin (7.5 μM) and menadione (2 μM) induced a similar extent of reactive oxygen species production in AML12 cells. The relative potencies of cytoprotection in vitro were in a descending order of curcumin>menadione>( ) Sch B, which were parallel to the extent of stimulation in cellular reduced glutathione level. We further examined their hepatoprotection in vivo. Pretreatment with Sch B (800 mg/kg) and curcumin (737 mg/kg), but not menadione (344 mg/kg), protected against CCl 4 toxicity, with the degree of protection afforded by Sch B being much larger than that of curcumin. The attenuated hepatoprotection afforded by curcumin may be attributed to its low bioavailability in vivo. This postulation is supported by the findings that intraperitoneal injections of Sch B (400 mg/kg) and curcumin (368 mg/kg) and the long term, low dose treatment with Sch B (20 mg/kg/d 15) and curcumin (18 mg/kg/d 15) induced glutathione antioxidant response and hepatoprotection to similar extents in vivo. The inability of menadione to induce hepatoprotection may be related to its extensive intestinal metabolism and/or hepatotoxicity. Taken together, prooxidants can invariably induce the glutathione antioxidant response and confer cytoprotection in vitro. Whether or not the prooxidant can produce a similar response in vivo would depend on its bioavailability and potential toxic effect.

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Prooxidant-Induced Glutathione Antioxidant Response in Vitro and in Vivo: A Comparative Study between Schisandrin B and Curcumin

Leong

Chiu

2012

Biological & Pharmaceutical Bulletin

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sch B was prepared as previously described [9]. Curcumin, EGCG, tBHP, 5,5'-Dithiobis(2-nitrobenzoic acid) (DTNB), GSH, GSSG, NADPH and NAD were purchased from Sigma Chemical Co. (St. Louis, MO, USA).…”

Section: Chemicalsmentioning

confidence: 99%

“…Twenty-four hours after CCl 4 administration, rats were phenobarbtial-anesthetized and heparinized blood samples were drawn by cardiac puncture, and animals were then killed by cardiac excision. Plasma samples were obtained by centrifuging whole blood samples at 1,500 ×g for 10 min at 4 o C. The extent of hepatic oxidative damage was assessed by the measurement of plasma sorbitol dehydrogenase (SDH) activity as described previously [9]. The extent of protection against CCl 4 hepatotoxicity was estimated using the equation:…”

Section: Hepatotoxicitymentioning

confidence: 99%

Schisandrin B Enhances Hepatic/Myocardial Glutathione Regeneration Capacity and Protects Against Oxidant Injury in Rat Livers and Hearts

Leong¹,

Chen²,

Ko³

2013

TONUTRAJ

View full text Add to dashboard Cite

Abstract:The effects of schisandrin B (Sch B), curcumin, epigallocatechin gallate (EGCG) and resveratrol on the glutathione regeneration capacity (GRC) of liver and heart tissues ex vivo were examined in relation to their protective effects against CCl 4 hepatotoxicity in vivo and myocardial ischemia/reperfusion (I/R) injury ex vivo in rats. Results indicated that Sch B, but not curcumin, EGCG or resveratrol, caused hepatic and myocardial GRC enhancement, which was paralleled by its protection against CCl 4 hepatotoxicity and myocardial I/R injury. Although resveratrol and curcumin failed to increase the GRC of liver/heart tissues, they did protect against CCl 4 hepatotoxicity and myocardial I/R injury. In conclusion, the Sch B-induced enhancement of GRC ex vivo correlated well with its ability to protect tissues against oxidant injury in vivo/ex vivo, suggesting that the GRC assay can be used as a monitor of tissue protection for compounds stimulating glutathione redox cycling. However, phytochemicals can also protect against oxidant injury through various other mechanisms.

show abstract

“…Inhalation of baicalin showed an inhibition of airway hyperresponsiveness (Dong et al, 2005). Also, Schizandrae Fructus and one of its components (schizandrin), were reNatural Products and Airway Mucin Production 397 ported to have various biological effects including free a radical scavenging effect (Li et al, 1990), hepatoprotective effect (Liu, 1989;Chiu et al, 2003) and to offer protection of neuronal cells from excitotoxicity (Kim et al, 2004). On the other hand, it was previously reported that baicalin and schizandrin inhibited ATP-stimulated mucin release from airway goblet cells, although baicalein did not affect ATP-stimulated airway mucin release in studies conducted by our group (Lee et al, 2003;Heo et al, 2006;Heo et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Effects of Baicalin, Baicalein and Schizandrin on Airway Mucin Production Induced by Epidermal Growth Factor and Phorbol Ester

Lee¹,

Lee²,

Kim³

et al. 2010

Biomolecules and Therapeutics

View full text Add to dashboard Cite

-We conducted this study to investigate whether baicalin, baicalein or schizandrin significantly affect MUC5AC mucin production induced by epidermal growth factor (EGF) or phorbol ester (PMA) in human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of baicalin, baicalein or schizandrin for 30 min and then stimulated with EGF or PMA for 24 h, respectively. MUC5AC mucin protein production was measured by ELISA. The results were as follows: (1) Baicalin was found to inhibit the production of MUC5AC mucin protein induced by both EGF and PMA. (2) Baicalein, the aglycone of baicalin, also inhibited MUC5AC mucin production. (3) Schizandrin, derived from Schizandrae Fructus, inhibited MUC5AC mucin production by the same inducers. These results suggest that baicalin, baicalein and schizandrin can regulate the production of mucin protein by directly acting on human airway epithelial cells.

show abstract

Hepatoprotective mechanism of schisandrin B: role of mitochondrial glutathione antioxidant status and heat shock proteins

Cited by 73 publications

References 36 publications

Prooxidant-Induced Glutathione Antioxidant Response <i>in Vitro</i> and <i>in Vivo</i>: A Comparative Study between Schisandrin B and Curcumin

Prooxidant-Induced Glutathione Antioxidant Response <i>in Vitro</i> and <i>in Vivo</i>: A Comparative Study between Schisandrin B and Curcumin

Schisandrin B Enhances Hepatic/Myocardial Glutathione Regeneration Capacity and Protects Against Oxidant Injury in Rat Livers and Hearts

Effects of Baicalin, Baicalein and Schizandrin on Airway Mucin Production Induced by Epidermal Growth Factor and Phorbol Ester

Contact Info

Product

Resources

About