Hepatoprotective effects of green Capsicum annum against ethanol induced oxidative stress, inflammation and apoptosis in rats

Das, Moumita; Basu, Subhashree; Banerjee, Bhaswati; Sen, Anurupa; Jana, Kuladip; Datta, Gouriprosad

doi:10.1016/j.jep.2018.08.019

Cited by 21 publications

(19 citation statements)

References 45 publications

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“…Coadministration of ethanol extract of Alocasia indica tuber significantly recovered the SOD activity to normal level in alcohol-intoxicated mice [30]. Similarly, extracts from Portulaca oleracea and green Capsicum annuum obviously enhanced the GSH-Px activity in alcohol-intoxicated rats [31,32]. Previous reports have also shown that MLE elevated the activity of SOD in chronic alcohol administration mice [17].…”

Section: Discussionmentioning

confidence: 74%

Mulberry leaves extract ameliorates alcohol-induced liver damages through reduction of acetaldehyde toxicity and inhibition of apoptosis caused by oxidative stress signals

Liang¹,

Yang²,

Teng³

et al. 2021

Int. J. Med. Sci.

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Mulberry leaves (Morus alba L.), which are traditional Chinese herbs, exert several biological functions, such as antioxidant, anti-inflammation, antidiabetic, and antitumor. Alcohol intake increases inflammation and oxidative stress, and this increase causes liver injury and leads to liver steatosis, cirrhosis, and hepatocellular carcinoma, which are major health problems worldwide. Previous report indicated that mulberry leaf extract (MLE) exited hepatoprotection effects against chronic alcohol-induced liver damages. In this present study, we investigated the effects of MLE on acute alcohol and liver injury induced by its metabolized compound called acetaldehyde (ACE) by using in vivo and in vitro models. Administration of MLE reversed acute alcohol-induced liver damages, increased acetaldehyde (ACE) level, and decreased aldehyde dehydrogenase activity in a dose-dependent manner. Acute alcohol exposure-induced leukocyte infiltration and pro-inflammation factors, including cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), were blocked by MLE in proportion to MLE concentration. MLE prevented alcohol-induced liver apoptosis via enhanced caveolin-1 expression and attenuated EGFR/STAT3/iNOS pathway using immunohistochemical analysis. ACE induced proteins, such as iNOS, COX-2, TNF-α, and IL-6, and inhibited superoxide dismutase expression, whereas co-treated with MLE reversed these proteins expression. MLE also recovered alcohol-induced apoptosis in cultured Hep G2 cells. Overall, our findings indicated that MLE ameliorated acute alcohol-induced liver damages by reducing ACE toxicity and inhibiting apoptosis caused by oxidative stress signals. Our results implied that MLE might be a potential agent for treating alcohol liver disease.

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Section: Discussionmentioning

confidence: 74%

Mulberry leaves extract ameliorates alcohol-induced liver damages through reduction of acetaldehyde toxicity and inhibition of apoptosis caused by oxidative stress signals

Liang¹,

Yang²,

Teng³

et al. 2021

Int. J. Med. Sci.

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“…Ethanol is metabolized into acetaldehyde by alcohol metabolizing enzymes, including alcohol dehydrogenase (present in cytosol), CYP2E1 (present in microsomes), and catalase (CAT) (present in peroxisomes). The acetaldehyde is further oxidized into acetic acid by aldehyde dehydrogenase in the mitochondria [8]. Chronic alcohol consumption induces an increase in cellular nicotinamide adenine dinucleotide hydrate concentration and acetaldehyde dehydrogenase activity, which leads to severe free fatty acid overload, triglyceride accumulation, and subsequent steatosis in hepatic tissue [9].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats

et al. 2019

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(1) Background: Graptopetalum paraguayense E. Walther is a traditional Chinese herbal medicine. In our previous study, 50% ethanolic G. paraguayense extracts (GE50) demonstrated good antioxidant activity. (2) Methods: To investigate the hepatoprotective effects of GE50 on ethanol and carbon tetrachloride (CCl4) co-induced hepatic damage in rats, Sprague–Dawley rats were randomly divided into five groups (Control group; GE50 group, 0.25 g/100 g BW; EC group: Ethanol + CCl4, 1.25 mL 50% ethanol and 0.1 mL 20% CCl4/100 g BW; EC + GE50 group: Ethanol + CCl4 + GE50; EC + silymarin group: ethanol + CCl4 + silymarin, 20 mg/100 g BW) for six consecutive weeks. (3) Results: Compared with the control group, EC group significantly elevated the serum aspartate aminotransferase (AST), alanine aminitransferase (ALT), and lactate dehydrogenase (LDH). However, GE50 or silymarin treatment effectively reversed these changes. GE50 had a significant protective effect against ethanol + CCl4 induced lipid peroxidation and increased the levels of glutathione (GSH), vitamin C, E, total antioxidant status (TAS), and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and glutathione S-transferases (GST). Furthermore, in EC focal group, slight fat droplet infiltration was observed in the livers, while in the GE50 or silymarin treatment groups, decreased fat droplet infiltration. HPLC phytochemical profile of GE50 revealed the presence of gallic acid, flavone, genistin, daidzin, and quercetin. (4) Conclusions: The hepatoprotective activity of GE50 is proposed to occur through the synergic effects of its chemical component, namely, gallic acid, flavone, genistin, daidzin, and quercetin. Hence, G. paraguayense can be used as a complementary and alternative therapy in the prevention of alcohol + CCl4-induced liver injury.

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“…Besides, the absence of heavy metals also makes the vegetable safe for consumption (Das et al, 2016). AqCA are known to possess antioxidant, anti-inflammatory, alcohol dehydrogenase inhibitory, immuno-modulatory, and ethanolytic activity (Das et al, 2018;Wang et al, 2019), and might contribute to enhanced antioxidant status and renal cell protection against ethanol-induced oxidative stress and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Standardization of the extract with respect to the marker compound capsaicin was performed using High Performance Liquid Chromatography (UFLC, Shimadzu, Japan) as presented in our previous work (Das et al., 2018).…”

Section: Methodsmentioning

confidence: 99%

Renoprotective effect ofCapsicum annumagainst ethanol‐induced oxidative stress and renal apoptosis

Das¹,

Basu²,

Banerjee

et al. 2020

J. Food Biochem.

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The present study explored the ameliorative potency of aqueous extract of Capsicum annum (AqCA), against oxidative imbalance and renal toxicity induced by ethanol. Randomly grouped male Wistar rats (n = 6), were marked as ethanol-treated (2 g/kg bw, i.p.), CA 125 (125 mg/kg bw, i.p.), CA 250 (250 mg/kg bw, i.p.), ethanol pre-treated with CA (similar doses), and control (0.5 ml normal saline, i.p.), and treated for 30 consecutive days. Biochemical analysis of tissue and serum parameters was performed, along with histopathological and histochemical studies. Also, we performed TUNEL assay and western blotting for our experimental groups. Statistical analysis revealed significant (p ≤ .001) alteration in the levels of antioxidant enzymes, serum urea, creatinine, pro-inflammatory cytokines, and cleaved caspases, along with histopathological alterations in the ethanol-treated group. Prior treatment with AqCA prevented ethanol-induced alterations in tissue and serum parameters. These findings indicate that the extract of CA can protect renal cells from ethanol-induced damage by inhibiting oxidative stress, inflammatory response, and apoptosis. Practical applications Chronic alcohol consumption is a major public health concern that leads to various diseases and social problems as well. It affects both the affluent and non-affluent society equally. Alcohol (ethanol) is a renowned hepato-toxicant and a well-documented risk factor for oxidative stress, with less known effect on the kidney. Thus, it is essential to investigate the effect of alcohol metabolism on the kidney to find a remedy to prevent it. The present investigation depicts the anti-oxidative and antiinflammatory role of Capsicum annum against ethanol-induced renal damage. The outcome of this study can be utilized in the future for phytotherapeutic herbal drug formulation. Besides, the bioactive components identified in the study can be further explored by researchers or pharmaceutical corporates for potential therapeutic purpose against renal impairment. 2 | MATERIAL S AND ME THODS 2.1 | Chemicals Ethanol was purchased from Merck, India. All other reagents used in the experiment were of analytical grade and were purchased either from Sigma-Aldrich, USA, or HiMedia Laboratories Pvt. Ltd., Mumbai, India. All Kits were purchased from Span Diagnostics Pvt. Ltd. TUNEL assay was performed using antibodies (Roche, Germany). Antibodies against Caspase-8, Caspase-9, and Caspase-3 were purchased from Cell Signaling Technology (Beverly, MA, USA). β-Actin antibodies and secondary antibodies (anti-mouse, anti-rabbit, and anti-goat) generated by Santacruz Biotechnology (Santacruz, CA, USA) were used. 2.2 | Plant material Green Capsicum annum L. (CA) was selected for the study and purchased from the local vegetable market approved by Kolkata Municipal Corporation from November 2018 to February 2019. The identity of the vegetable was confirmed by the Botanical Survey of India (BSI), Government of India, Ministry of Environment and Forests, Howrah, West Bengal, India. A herbar...

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Hepatoprotective effects of green Capsicum annum against ethanol induced oxidative stress, inflammation and apoptosis in rats

Cited by 21 publications

References 45 publications

Mulberry leaves extract ameliorates alcohol-induced liver damages through reduction of acetaldehyde toxicity and inhibition of apoptosis caused by oxidative stress signals

Mulberry leaves extract ameliorates alcohol-induced liver damages through reduction of acetaldehyde toxicity and inhibition of apoptosis caused by oxidative stress signals

Antioxidant Activity of Graptopetalum paraguayense E. Walther Leaf Extract Counteracts Oxidative Stress Induced by Ethanol and Carbon Tetrachloride Co-Induced Hepatotoxicity in Rats

Renoprotective effect ofCapsicum annumagainst ethanol‐induced oxidative stress and renal apoptosis

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