Hepatoprotective effect of Red Sea sponge extract against the toxicity of a real-life mixture of persistent organic pollutants

Moneam, Nehad M Abd El; Shreadah, Mohamed A.; El-Assar, Samy A.; Voogd, Nicole J. de; Nabil-Adam, Asmaa

doi:10.1080/13102818.2018.1441747

Cited by 15 publications

(12 citation statements)

References 43 publications

(66 reference statements)

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“…However, the optimal bioaccumulation capacity is at about 6 chlorines, probably because higher chlorinated congeners (esp. octa-) are so poorly water soluble so that their bioavailability is low [49,50]. α,β -HCH residues were found at high concentration in Siganus rivulatus muscle samples collected from El-Mex Bay with an average of 28.31 and 28.46 ng/g, dry weight, respectively and are much more than their corresponding values for fish collected from Matrouh coast (2.183 and 5.96 ng/g, dry weight, respectively).…”

Section: Chemical Analysismentioning

confidence: 92%

Chemical and Biochemical Evaluation of Marine Pollution by Polychlorinated Biphenyls and Pesticides in Two Regions Along the Egyptian Mediterranean Sea

Sheradah¹

2018

IJEMA

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The main objective of this paper is to understand the importance of biochemical biomarkers in assessing marine pollution by Polychlorinated biphenyls (PCBs) and Pesticides. Methods: The oxidative stress and antioxidant response as well as ultrastructural changes of both liver and muscle tissues of Siganusrivulatus collected from El-Mex Bay as a polluted region and Marsa Matrouh as a control region far from land-based sources of pollution have been investigated by using standard methods. In general, the results cleared out that El-Mex Bay was much more polluted by polychlorinated biphenyls (PCBs) and pesticides compared to Matrouh area. The concentration range of total polychlorinated biphenyls (PCBs) and pesticides in sediment samples collected from Matrouh coast was 16.29-32.58 ng/g, dry weight and 107.62-149.11ng/g, dry weight, respectively. On contrast, the range in sediment samples collected from El-Mex Bay was 217.12-255.33 ng/g, dry weight and 663.73-778.7ng/g, dry weight, respectively. Biomarkers studies revealed that the induction of antioxidant systems in response to oxidative stress and the increase in lipid peroxidation in fish collected from the polluted area were good indicators for the pollution load" in the present study. On the meantime, the present study revealed that ultrastructural changes serve as a biomarker of stress in aquatic environment. Moreover, the results suggested that chemical pollution, on the other hand, is capable of inducing morphological alteration in liver of fish collected from polluted area.

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Section: Chemical Analysismentioning

confidence: 92%

Chemical and Biochemical Evaluation of Marine Pollution by Polychlorinated Biphenyls and Pesticides in Two Regions Along the Egyptian Mediterranean Sea

Sheradah¹

2018

IJEMA

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“…Total Phenolic Contents: Phenolic compounds have been shown to possess important biological activities based on their structural characteristics [1][2][3][4][5][6][7][8][9]. There are concerns about using synthetic phenolic antioxidants such as butylated hydroxytoluene…”

Section: Phenolic Compoundsmentioning

confidence: 99%

“…There is an increasing interest toward research on flavonoids occurring in various sources of marine organisms including sponges and their associated bacteria due to their multipurpose health benefits such as free-radical scavenging property, anticancer activity, antiviral properties etc. [1][2][3][4][5][6][7][8][9]57]. Highly significant quantities of flavonoid contents were found in crude extract of the tested bacterial isolates equivalents of (+catechin).…”

Section: Flavonoid Compoundsmentioning

confidence: 99%

“…Sponges (phylum Porifera) are known to be extremely rich sources of bioactive compounds, mainly of secondary metabolites of pharmaceutical and medical relevance. The development of secondary metabolites from the Red Sea sponges offer a potential for producing novel drugs and prototypes and has been a subject of intense research due to their immense bioactive nature [1][2][3][4][5][6][7][8][9]. They are also well known to be hosts for a large community of microorganisms [1,10].…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Phylogenetic Analysis of the Microbial Community Associated with the Red Sea Sponge Ircinia Echinata and Biological Evaluation of their Secondary Metabolites

Shreadah¹,

Monem²,

Yakout³

et al. 2018

BJSTR

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The present study was undertaken to provide information about bioactive compounds of the microbial community associated with Ircinia echinata marine sponge collected from the Red Sea. This work investigated the antioxidant and cytotoxicity properties of the tested extracted crude extract. Sponge was taxonomically identified as Ircinia echinata. Moreover, taxonomic and phylogenetic analyses of the bacteria on the basis of 16S rRNA gene sequences showed that dominant phylum was proteobacteria. The results of HPLC analysis revealed that the bacterial crude extracts exhibited highly significant quantities of total phenolic contents in crude extracts of the two tested bacterial isolates. The highest level of total phenolic contents was measured in the extract was shown in extracts HA-MS-41 (3.238±0.03 mg gallic equivalent/ml). Whereas, the lowest value was shown in extract HA-MS-118 (2.946±0.10 mg gallic equivalent/ml).The results of the current study cleared out that extract HA-MS-41[JX484800] contained gallic and tannic acids (1.084 and 1.413 mg/ml), whereas extract HA-MS-118 [JX484801] contained gallic (1.005 mg/ml) but didn't contain tannic acid. The highest level of total flavonoid content was measured in the extract HA-MS-41 extract (5±0.23 mg catechin equivalent / ml). Whereas, much lower value was shown in the HA-MS-118 extract (2.5±0.26 mg catechin equivalent / ml). Carotenoid compounds significantly varied among the two bacterial extracts; the highest total carotenoid concentration was observed in the extract HA-MS-41 (1.165µg/ml). Whereas, lower valuesobserved in the extract HA-MS-118 (0.185 µg/ml). In the meantime, GC/MS profiling for the ethyl acetate extract of the studied sponge-associated bacteria revealed the presence of various phytochemical compounds having different biological activities. Results of FTIR spectroscopic studies have revealed the presence of various chemical constituents in ethyl acetate extract of the HA-MS-41 and HA-MS-118 bacterial isolates.The results of the elemental analysis indicated that the bacterial extracts contained major elements like Na, Mg, Al, Si, S, K, Ca, P and Cl, besides the major elemental constituents in all living organisms; C, N and O. The results showed considerable concentrations of many halides (Fe, Cu, Zn and Br). DPPH scavenging activity in terms of IC50 values was shown to be high in case of the extract HA-MS-41 (1.045 mg/ml) and in case of the extract HA-MS-118 (1. 450 mg/ml). IC50 values of the antioxidant activity of bacterial extracts using NO assay for the tested two extracts were 5.98 and 6.222 %. Moreover, potent antioxidant activity using ABTS+ assay with percentages inhibition of IC50 = 3.404 and 1.64 mg/ml for HA-MS-41and HA-MS-118, respectively. In the present study highly significant total antioxidant capacity values was also obtained by using phosphomolybdenum assay in the bacterial extract suggesting the presence of effective antioxidants in the bacterial extract.The high potency of these isolates is mainly attributed to the presence of vari...

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“…Sponges have long been known as a source for natural products of pharmaceutical and medical relevance [1]. The Red Sea sponges have been the focus of much recent interest [2][3][4][5][6][7][8][9] because they form close associations with a wide variety of microorganisms such as viruses, bacteria, archaea, fungi, protozoa,…etc. This complex micro biota makes marine sponges of particular interest to microbial ecology studies and also offers a potentially invaluable source of novel genes and gene products for biotechnological applications [10].…”

Section: Introductionmentioning

confidence: 99%

Sponge-Associated Bacteria: Isolation, Phylogenetic Analysis and Biotechnological Potential

MA¹

2019

BJSTR

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Marine sponge Hyrtios erectus is a source of numerous toxic secondary metabolites, which are considered as products of the symbiotic species associated with the sponge. Sponge-associated bacteria have great potential in developing marine pharmaceutical industry since they are capable of synthesizing numerous bioactive metabolite compounds. This study aimed to isolate the associated bacteria, characterize their different secondary metabolite , and investigate their potential antioxidant and cytotoxicity activities. Sponge was taxonomically identified as Hyrtios erectus. Moreover, taxonomic and phylogenetic analyses of the bacteria on the basis of 16S rRNA gene sequences showed that the dominant two phyla were Firmicutes and proteobacteria. The results of HPLC analysis revealed that Chlorogenic acid, Coffeic acid, and 3,5-Dicaffeoyl quinic acid were the main phenolic compounds present in the HA-MS-22 [JX494759] crude extract, whereas Chlorogenic acid and 3,5-Dicaffeoyl quinic acid were present in the HA-MS-21 [JX494761] crude extract. Moreover, extract HA-MS-22 [JX494759] did not contain gallic but contained tannic. In the meantime, extract HA-MS-21 [JX494761] contained both gallic and tannic acids. The highest level of total phenolic contents was shown in extracts HA-MS-22 (3.702 ±0.10 mg gallic equivalent/ml).Whereas, the lowest value was shown in extract HA-MS-23 (3.015±0.045 mg gallic equivalent/ml). Significantly high concentration of total flavonoids (11.16±0.46 mg catechin equivalent/ml). were measured in the ethyl acetate JX494761 extract. The JX494759 crude extract contained also considerably high contents of total flavonoids (7.61±0.003 mg catechin equivalent/ml). Carotenoid compounds significantly varied among the two bacterial extracts; the highest total carotenoid concentration was shown in extract HA-MS-22 (0.048µg/ml). Whereas, lower values were shown in extracts JX494761 (4.098µg/ml). In the meantime, GC/MS profiling for the ethyl acetate extract of the studied sponge-associated bacteria revealed the presence of various phytochemical compounds having different biological activities. Results of FTIR spectroscopic studies have revealed the presence of various chemical compounds. The presence of O

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Hepatoprotective effect of Red Sea sponge extract against the toxicity of a real-life mixture of persistent organic pollutants

Cited by 15 publications

References 43 publications

Chemical and Biochemical Evaluation of Marine Pollution by Polychlorinated Biphenyls and Pesticides in Two Regions Along the Egyptian Mediterranean Sea

Chemical and Biochemical Evaluation of Marine Pollution by Polychlorinated Biphenyls and Pesticides in Two Regions Along the Egyptian Mediterranean Sea

Isolation, Phylogenetic Analysis of the Microbial Community Associated with the Red Sea Sponge Ircinia Echinata and Biological Evaluation of their Secondary Metabolites

Sponge-Associated Bacteria: Isolation, Phylogenetic Analysis and Biotechnological Potential

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