Hepatoprotective effect of Quercetin supplementation against Acrylamide-induced DNA damage in wistar rats

Abstract

BackgroundQuercetin (QR), is a polyphenolic flavonoid compound which is found in large amounts in certain foods, and protects against oxidative stress. The current study was conducted to determine whether Quercetin can possibly exert hepatoprotective and antioxidant activity against acrylamide (ACR) induced toxicity in rats.MethodsFour groups of Wistar rats consisting of six rats each: (i) control group; (ii) ACR treated group (50 mg/kg bw); (iii) QR group: rats were treated with QR (10 mg/kg bw); (iv) QR (10 mg/kg bw) was given i.p. for 5 days followed by ACR (50 mg/kg bw) on 5th day (single dose).ResultsACR caused an elevation in 8-OH guanosine level and a reduction in Glutahione S-transferase (GST) activity. Administration of QR significantly protected liver tissue against hepatotoxic effect of acrylamide from amelioration of the marker enzyme (p < 0.05) and DNA damage (p < 0.01) as evident by comet assay and, besides some indices of histopathological alterations.ConclusionIt is concluded that QR could protect the liver against DNA damage induced by ACR probably is thus capable of ameliorating ACR-induced changes in the rat livers.

Antioxidant, hepatoprotective, genoprotective, and cytoprotective effects of quercetin in a murine model of arthritis

“…ROS is capable to cross cell membranes and react with DNA in the nucleus, 63 which contribute to changes in the cells such as increased apoptosis and proliferation, as well as in the genetic material such as rising mutation rates and genome instability. 65,67 We found that the untreated arthritic rats had higher levels of DNA damage, a match to the findings of Altindag et al, 13 where RA patients had increased DNA damage compared with healthy subjects. 13 However, we observed a significant reduction in DNA damage in groups treated with quercetin.…”

“…These results indicate that quercetin scavenges ROS, thereby, decreasing DNA breaks and oxidized bases. In a study conducted by Ansar et al, quercetin decreased DNA damage in rats with acrylamide‐induced toxicity, suggesting that the antioxidant effects of this flavonoid are due to high membrane diffusion which aids the removal of oxyradicals from the lipid bilayer . Furthermore, the chelation of metal ions by quercetin is due to its pentahydroxyl structure, which eliminates lipid alkoxyl and peroxyl radicals .…”

Geroprotective and Radioprotective Activity of Quercetin, (-)-Epicatechin, and Ibuprofen in Drosophila melanogaster

“…Firstly, well-known anti-oxidant properties of quercetin and (-)-epicatechin provide the protection of cellular macromolecules from damages and predict the mitochondrial dysfunction (Khan et al, 2016). In this way, studied drugs demonstrated benefit action against the development of aging-related pathologies including neurodegeneration (Shay et al, 2015; Barreca et al, 2016; Elumalai and Lakshmi, 2016), cardiovascular and liver diseases (Ansar et al, 2016; Cheserek et al, 2016), diabetes (Shay et al, 2015; Haddad and Eid, 2016), cancer (Shay et al, 2015; Khan et al, 2016). …”

Protective role of quercetin against manganese-induced injury in the liver, kidney, and lung; and hematological parameters in acute and subchronic rat models

“… 25 Qct has a protective effect against acrylamide-induced oxidative stress in rats. 26 Recently, the protective role of Qct against hemotoxic and immunotoxic effects of furan in rats was reported. 27 Qct has protective effects against hepatic injury by increasing plasma antioxidant capacity.…”