Hepatoprotective effect oftrans-Chalcone on experimentally induced hepatic injury in rats: inhibition of hepatic inflammation and fibrosis

Abstract

The current study investigated the hepatoprotective effect of trans-Chalcone in carbon tetrachloride (CCl4) and paracetamol (PCM) induced liver damage in rats. Administration of CCl4 and PCM (1 mL/kg, i.p., 3 days, and 2 g/kg, p.o., single dose, respectively) produced hepatic injury. Ponderal changes (percent change in body mass and relative liver mass) and biochemical parameters (serum ALT, AST, ALP, bilirubin) were estimated. The markers of oxidative and nitrosative stress (TBARS, reduced GSH, nitrite and nitrate), hepatic fibrosis (TGF-β1, collagen content), hepatic inflammation (TNF-α), and histopathological study were evaluated. trans-Chalcone (5, 10, and 20 mg/kg, i.p.) was found to be beneficial as demonstrated by significant reversal of liver histology by perceptible reduction of inflammatory cell infiltration with regenerative changes in hepatocytes. Improvement in percent change in body mass and significant reduction in relative liver mass were observed. Marked reduction in serum levels of ALT, AST, ALP, and bilirubin were noted. Decreases in TBARS and nitrites and nitrates and increases in reduced GSH levels were noted. Hepatic fibrosis and inflammation were significantly decreased. The findings indicate a novel hepatoprotective role for trans-Chalcone by improving hepatic injury by possible actions such as anti-oxidant, anti-nitrosative, anti-fibrotic, and anti-inflammatory. Hence, it can be used as promising hepatoprotective agent.

Hepatoprotectivity of Panduratin A against liver damage: In vivo demonstration with a rat model of cirrhosis induced by thioacetamide

“…Additionally, the profibrogenic cytokine TGF‐β1 can up‐regulate the deposition of extracellular matrix (ECM) . Studies on some chalcones recently reported on their hepatoprotectivity against chemical intoxication via inhibition of hepatic fibrosis markers . In this research, the fibrogenesis markers, PDGF and TGF‐β1 were down‐regulated in the animals treated with the isolated chalcone PA in the same manner as the Silymarin‐treated animals in contrast to those of cirrhotic controls (Fig.…”

Therapeutic Potential of Flavonoids in Pain and Inflammation: Mechanisms of Action, Pre-Clinical and Clinical Data, and Pharmaceutical Development

“…Trans-chalcone (1,3-diphenyl-2-propen-1-one) is a flavonoid precursor that can be obtained from plants such as Piper methysticum (Kava-Kava), Aniba riparia and Didymocarpus corchorijolia [145]. Trans-chalcone presents in vivo antioxidant effects [42,145] and anti-hyperalgesic mechanisms through the inhibition of leukocyte recruitment, IL-1β, TNF-α, and IL-6 production, and NLRP3 inflammasome activation in macrophages stimulated with MSU crystals [42]. It also inhibits VEGF and ICAM-1 expression and activation of STAT3 and NF-κB [41], indicating that part of its analgesic mechanism is related to the blockade of NF-κB-dependent pro-inflammatory mediators.…”

Effect of trans-chalcone on hepatic IL-8 through the regulation of miR-451 in male rats

“…Moreover, as shown by Singh et al (2016a), this chalcone reduced markers of liver injury (AST and ALT) and infl ammation (TNF-α) in toxicant induced liver injury. Hence, anti-infl ammatory eff ect of trans-chalcone may be involved in its hepatoprotective eff ect (Singh et al 2016a).…”

“…Hepatoprotective eff ect of trans-chalcone and its ability to reduce serum levels of AST and ALT has been shown by Karkhaneh et al (2016). Moreover, as shown by Singh et al (2016a), this chalcone reduced markers of liver injury (AST and ALT) and infl ammation (TNF-α) in toxicant induced liver injury. Hence, anti-infl ammatory eff ect of trans-chalcone may be involved in its hepatoprotective eff ect (Singh et al 2016a).…”

“…Chalcones exert a broad range of biological activities such as anti-infective, anti-infl ammatory (Nowakowska 2007), anti-oxidant (Singh et al 2016a,b), anti-cancer (Katsori and Hadjipavlou-Litina 2009), cytoprotective (Sikander et al 2011), anti-obesity (Karkhaneh et al 2016), and anti-diabetic (Najafi an et al 2010). Trans-chalcone is a simple structure chalcone and also an open chain fl avonoid (Batovska and Todorova 2010;Singh et al 2016a) which has anti-fi brotic, antioxidant, and anti-infl ammatory eff ects (Singh et al 2016a). However, the mechanism of its anti-infl ammatory eff ect is poorly understood and also the eff ect of this chalcone on miR-451/IL-8 pathway has not been investigated.…”