Hepatoprotective and Nephroprotective Effect of Curcumin Against Copper Toxicity in Rats

Abstract

Curcumin is a natural anti-inflammatory and antioxidant with several potential health benefits. Although it has been examined in several metals toxicity studies, but its role in the protection against copper toxicity has not been investigated. In this study; the detoxification and antioxidant effect of curcumin were examined to determine its prophylactic/therapeutic role experimentally in rats. Forty albino rats were divided into five groups; control, CuSO4 (4 mg/kg body weight), curcumin (80 mg/kg body weight), curcumin post-treatment (CuSO4 for 15 days followed by curcumin for the next 15 days) and curcumin co-treatment (CuSO4 plus curcumin for 30 days). All rats were treated orally by stomach tube for 30 days/once a day. Changes were observed in hepatic marker enzymes such as: aspartate aminotransferase (AST), alanine transaminase-(ALT), alkaline phosphatase (ALP) and gamma-glutamyltransferase (GGT), besides the serum total protein, urea and creatinine. Concentration of liver and kidney antioxidants such as: catalase (CAT), superoxide dismutase (SOD), reduced glutathione-(GSH) and malondialdehyde (MDA) were measured. An increased in the activities of liver marker enzymes, urea, creatinine and the MDA contents were detected after exposure to CuSO4. Meanwhile, the activities of serum total protein, hepatic and renal antioxidants were decreased. Changes in all biochemical parameters were alleviated by the post-treatment and co-treatment of curcumin. Our finding suggests that the curcumin showed protective effects on CuSO4-induced hepatotoxicity and nephrotoxicity.

The Potential Ameliorative Effect of Nano-Zinc and Zinc Against Copper Hepatorenal Toxicosis in Rats

“…Histopathologically, the kidneys showed severe tubular necrosis with renal casts and severe interstitial nephritis associated with inflammatory cells infiltration. Similar results were reported by Hashish and Elgaml (2016). The NZn treated rats also, showed a significant increase in serum hepatic enzymes (AST, ALT, ALP and GGT), total and indirect bilirubin with a significant decrease in total protein and albumin.…”

“…Cu is a catalyst that leads to induction of oxidative stress and formation of reactive oxygen species (ROS) leading to cellular, DNA and lipid damage. The main target organs for Cu toxicity are liver and kidney leading to hepato-renal toxicity (Hashish and Elgaml, 2016). Zinc (Zn) is an essential metal which plays an important role in several biochemical and cellular functions (Fang et al, 2002) as the stabilization of biological membranes and protecting them from damage , preventing free radical formation, correcting the immunity, replication and transcription of DNA and intercellular signaling (Sandstead et al, 2007 andShah, 2011).…”

Enzyme-Catalyzed Glycosylation of Curcumin and Its Analogues by Glycosyltransferases from Bacillus subtilis ATCC 6633

“…Besides these usages, curcumin exerts promising pharmacological properties, such as anti-oxidant, anticancer, anti-inflammatory and antifibrinolytic effects [3][4][5]. Thus, it has long been believed to be a potential protective natural compound against cardiac diseases, and to possess hepatoprotective and nephroprotective activities [6]. Over the past several decades, extensive research has demonstrated its possible action mechanisms, such as inhibiting experimental allergic encephalomyelitis in the treatment of multicentric sclerosis [7], and as a sarcoplasmic/endoplasmic reticulum calcium pump against cystic fibrosis [8].…”

Evaluation of the renoprotective effect of nano turmeric against toxic dose of copper sulfate: Role of vascular cell adhesion molecule‐1, kidney injury molecule‐1, and signal transducer and activator of transcription 3 protein expressions

“…Herein, CS‐induced oxidative renal tissue damage by elevating the serum urea, creatinine, and uric acid levels. Hashish and Elgaml detailed the changes in serum urea creatinine levels in rats after CS administration. TM, NTM, or DSM administration successfully decreased the previous levels.…”

“…Rats were allowed to acclimatize for 1 week before the experiment commenced, and then they were divided into five groups of 10 each. Group I (untreated control) was injected with carboxymethylcellulose, group II (positive control) was administered CS orally (300 mg/kg), group III (CS+DSM) was administered CS and DSM (100 mg/kg), group IV (TM‐treated) was administered TM 80 mg/kg, and group V (CS+NTM80) was treated with CS and NTM (80 mg/kg). All treatments were administered simultaneously with CS daily for 7 days.…”