Hepatoprotective and Antioxidative Activities ofCornus officinalisagainst Acetaminophen-Induced Hepatotoxicity in Mice

Abstract

The fruit of Cornus officinalis Sieb. et Zucc. is commonly prescribed in Asian countries as a tonic formula. In this study, the hepatoprotective effect of ethanolic extracts of the fruit of C. officinalis (ECO) was investigated in a mouse model of acetaminophen- (APAP-) induced liver injury. Pretreatment of mice with ECO (100, 250, and 500 mg/kg for 7 days) significantly prevented the APAP (200 mg/kg) induced hepatic damage as indicated by the serum marker enzymes (AST, ALT, and LDH). Parallel to these changes, ECO treatment also prevented APAP-induced oxidative stress in the mice liver by inhibiting lipid peroxidation (MDA) and restoring the levels of antioxidant enzymes (SOD, CAT, and HO-1) and glutathione. Liver injury and collagen accumulation were assessed using histological studies by hematoxylin and eosin staining. Our results indicate that ECO can prevent hepatic injuries associated with APAP-induced hepatotoxicity by preventing or alleviating oxidative stress.

Therapeutic potential ofMoringa oleiferaextracts against acetaminophen-induced hepatotoxicity in rats

“…The increase in ALT is the most intense during the first 12 h (Chanda et al, 1995). Our experimental model of hepatotoxicity induced by 6 g APAP/kg bw demonstrated a significant rise of ALT and AST in the first 24 h. The present study correlates with other studies showing significant increases of ALT and AST values after administration of hepatotoxicants like APAP (Liu et al, 2012;Lee et al, 2012;Anwar et al, 2007).The normalization of the above enzymes activities seen in rats treated with the MO extracts indicates inhibition of liver cell injury and reduction of leakage of these enzymes into the blood. These results suggested that MO extracts reduced the toxicity due to elimination of the toxic products of APAP in rats.…”

Using random forest and decision tree models for a new vehicle prediction approach in computational toxicology

“…Liver toxicity is one of the most common toxicity endpoints measured since most xenobiotics are metabolised via the liver. Aspartate transaminase (AST) and alanine transaminase (ALT) are two such biomarkers associated with liver toxicity (Garg et al 2007;Lee et al 2012;Das et al 2011). …”

Amelioration of carbon tetrachloride- and paracetamol-induced hepatotoxicity in rats by <i>Ficus dalhousiae</i>

“…N-acetyl-p-benzoquinone imine (NAPQI) which is one of the metabolites of paracetamol after the later undergoes metabolism in the liver through the action of cytochrome P450 monooxygenase (Lee et al, 2012) is highly responsible for the toxic effects of paracetamol to liver (Farghaly and Hussein, 2010). Moreover, Somchit et al also suggested that NAPQI is involved in the formation of protein adducts through its action on DNA, proteins, cellular proteins, which in turn lead to the dysfunction and death of hepatocytes and finally liver necrosis (Somchit et al, 2005).…”