Hepatoprotective and antioxidant effects of fish oil on isoniazid-rifampin induced hepatotoxicity in rats

Basheer, Abdul Samad; Siddiqui, Aisha; Paudel, Yam Nath; Hassan, Md. Quamrul; Imran, Mohd; Najmi, Abul Kalam; Akhtar, Mohd

doi:10.1016/j.phanu.2017.01.002

Cited by 15 publications

(9 citation statements)

References 18 publications

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“…However, hepatotoxic control (1B) demonstrated degeneration in hepatocytes with vacuolation, inflammation and necrosis, which are also substantiated by previous researchers. 7,8 The present study revealed that diosmin at lower dose (100 mg/kg) with INH and RIF (1C) demonstrated protection of liver as apparent from mild degenerative changes in hepatocytes with inflammation and necrosis. However, higher dose of diosmin (200 mg/kg) demonstrated liver protection (1D) which is evident as normal morphology of hepatocytes without any inflammatory and necrosis changes.…”

Section: Discussionmentioning

confidence: 52%

“…The exact mechanism of liver injury associated with INH and RIF have not been investigated thoroughly, however it is reported that drug metabolites promote formation of reactive oxygen species (ROS) and augment cellular oxidative damage, inflammation and apoptosis of hepatocytes. 7,8 Most of the anti-tubercular drugs are lipid soluble and need hepatic phase I and phase II metabolism into more water-soluble metabolites that can be easily excreted. In this context, INH is metabolized by N-acetyltransferase in the liver to toxic intermediates such as acetylhydrazine and hydrazine, which are subsequently metabolized by cytochrome P450 2E1 (CYP2E1) to toxic reactive metabolites.…”

Section: Introductionmentioning

confidence: 99%

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Hepatoprotective potential of diosmin against hepatotoxic effect of isoniazid and rifampin in wistar rats

et al. 2023

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Objective The treatment of tuberculosis with isoniazid and rifampin is associated with hepatocellular damage. Therefore, the study was designed to evaluate the hepatoprotective potential of diosmin against hepatotoxic effect of isoniazid and rifampin in Wistar rats. Methods Hepatotoxicity was induced by administering isoniazid and rifampin (100 mg/kg), whereas diosmin was given as treatment control. Markers of liver function (ALT, AST, ALP and bilirubin), inflammatory cytokines (TNFα, IL-6 and IL-1β), apoptosis (caspase-3), oxidative stress parameters (LPO, GSH, CAT and SOD) and histological changes in liver were assessed in normal, hepatotoxic control and treatment groups. Results The administration of isoniazid and rifampin significantly increased markers of liver dysfunction (ALT, AST, ALP and bilirubin), cytokines (TNFα, IL-6 and IL-1β) and apoptosis (caspase-3). However, daily dosing of diosmin significantly reduced these markers of liver dysfunction, inflammatory cytokines and apoptosis to near normal levels. Additionally, markers of hepatocellular oxidative stress parameters were significantly altered as evident from increased LPO level and decreased endogenous antioxidants such as GSH, SOD and CAT in isoniazid-and rifampin-treated hepatotoxic group. It was observed that diosmin treatment reduced high levels of LPO and demonstrated significant improvement in antioxidant levels. Histological studies of liver also supported our biochemical findings, which are also manifested as diosmin treatment exhibited protection against hepatocellular degeneration and inflammation. Conclusion Results of the present study demonstrate hepatoprotective potential of diosmin against isoniazid-and rifampin-treated hepatotoxicity. Thus, we conclude that diosmin may be used along with anti-tubercular drugs (isoniazid and rifampin) in tuberculosis patients to overcome their hepatotoxic adverse effect.

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Section: Discussionmentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Hepatoprotective potential of diosmin against hepatotoxic effect of isoniazid and rifampin in wistar rats

et al. 2023

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“…Up to 40% of liver and kidney failure are traceable to side effects of conventional drugs administered in the management of ailments (Kosanam and Boyina, 2015). Specifically, hepato-renal injuries have been linked to oxidative assaults caused by specific bioactivated intermediates produced in biotransformation (Sharma and Sharma, 2015;Basheer et al, 2017). The use of plants for medication is an age-long practice that has proven effective (Sentman et al, 2006;Abirami et al, 2014).…”

Section: Resultsmentioning

confidence: 99%

Pergularia daemia (Apocynaceae) mitigates rifampicin-induced hepato-renal injury: potentials in the management of liver and kidney diseases

Ogunmoyole,

Fatile,

Johnson

et al. 2022

Int. J. Plant Based Pharm.

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Medicinal potentials of Pergularia daemia leaves in managing hepato-renal toxicity induced by rifampicin were investigated. Twenty-five (25) Wistar rats were randomly placed into five groups containing five animals each. All the animals, except group I, were orally exposed to 250 g/kg bwt rifampicin and administered different treatments. Specific liver and kidney biomarkers such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined. In addition, malondialdehyde (MDA), lipid profile, superoxide dismutase (SOD), catalase (CAT), as well as reduced glutathione (GSH) were determined in the serum, liver, and kidney homogenates of experimental animals. Results indicate that exposure to rifampicin caused significant depletion in SOD and CAT relative to the control animals. Lipid profile was deranged, while ALT, AST, ALP, urea, uric acid, bilirubin, creatine kinase, and MDA level were elevated by rifampicin exposure. All deranged biochemical indices, as well as distorted histoarchitecture, were restored dose-dependently after treatment with P. daemia. In conclusion, P. daemia ameliorated rifampicin toxicity on the liver and kidney as indicated in the restoration of all deranged biochemical and histopathological indices measured. Hence, it is a potential therapeutic agent that can be harnessed as the panacea to the menace of liver and kidney diseases.

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“…27 Metabolites of isoniazid, which play an essential role in liver injury by lipid peroxidation and DNA strand breaks. 28,29 The combination of R-H induced liver damage is intervened through oxidative damage to the hepatocyte, which caused the leakage of enzymes into vascular compartments and an increase in the malondialdehyde content which indicated the increased the level of lipid peroxidation. 25 The results of the present study showed a significant increase in the liver lipid peroxidation levels (appr.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Hepatoprotective Effect of Ethanolic Extract of Physali Ixocarpa Against Rifampicin-Isoniazid Induced Hepatotoxicity in Wistar Rats

Delhiraj¹,

Jaganathan²,

Vijayakumar³

2020

Int. Res. J. Pharm

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The liver is the primary organ that metabolizes the majority of the drug. Toxicity caused by these drugs to the liver is called hepatotoxicity. Hepatotoxicity is a major concern in tuberculosis therapy, especially Rifampicin - Isoniazid (R-H). Studies showed that these drugs induce oxidative stress in the liver. This study attempts to determine whether the ethanolic extract of Physalis ixocarpa (EEPI) protects against R-H induced hepatotoxicity in adult male Wistar rats. Adult male Wistar rats were divided into five groups (each group n=6 animals). Group I, control treated with normal saline (5ml/kg, b/w, p.o.). Group II, Hepatotoxicity induced by combination of R-H (each 50mg/kg, i.p.) administered up to 14 days. Group III and IV, EEPI (100 mg/kg & 200 mg/kg, b/w) were administered orally one hour before the R-H inducing agent up to 14 days. Group V, Silymarin (25 mg /kg, b/w., p.o.) was served as standard. After 14th days animals were allowed fast overnight and blood was collected through orbital puncture and animal was sacrificed then liver tissue was collected for biochemical analysis and histopathological studies. Our results show a significant reduction in the level of alkaline phosphate (ALP), alanine transaminase (ALT), aspartate transaminase (AST) and total bilirubin. Treatment with EEPI also showed a significant increase in the activity of antioxidant enzymes and decreased levels of malondialdehyde (MDA) in the liver. EEPI also reduced the macrovesicular steatosis and ballooning caused by the R-H. The present study demonstrates that administration of ethanolic extract of Physalis ixocarpa ameliorating hepatoprotective activity as evidenced by the biochemical and histopathological parameters.

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Hepatoprotective and antioxidant effects of fish oil on isoniazid-rifampin induced hepatotoxicity in rats

Cited by 15 publications

References 18 publications

Hepatoprotective potential of diosmin against hepatotoxic effect of isoniazid and rifampin in wistar rats

Hepatoprotective potential of diosmin against hepatotoxic effect of isoniazid and rifampin in wistar rats

Pergularia daemia (Apocynaceae) mitigates rifampicin-induced hepato-renal injury: potentials in the management of liver and kidney diseases

Evaluation of Hepatoprotective Effect of Ethanolic Extract of Physali Ixocarpa Against Rifampicin-Isoniazid Induced Hepatotoxicity in Wistar Rats

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