Hepatoprotective and antioxidant effect of Sphenocentrum jollyanum (Menispermaceae) stem bark extract against CCl4- induced oxidative stress in rats

Abstract

Methanolic stems bark extract of Sphenocentrum jollyanum (SBSJ) was evaluated for hepatoprotective and antioxidant activity against carbon tetrachloride (CCl 4)-induced hepatic damage in rats. The extract (50, 100 and 200 mg/kg) was administered orally to the animals with hepatotoxicity induced by CCl 4 (30%, 1.0 ml/kg). In vitro antioxidant potential of the extract was evaluated by using superoxide (O 2 .-) and hydrogen peroxide (H 2 O 2) radical scavenging assay. At a concentration of 50, 100 and 200 mg/kg, the extract showed a remarkable hepatoprotective and antioxidant activity against carbon tetrachloride (CCl 4)-induced liver injury in a concentration dependant manner. This was evident from significant reduction (P< 0.05) in serum marker enzymes, aspartate amino transferase (AST), alanine amino transferase (ALT), alkaline phosphatase (ALP), total bilirubin and lipid peroxidation. It was also observed that the extract of S. jollyanum stem bark conferred a significant (P < 0.05) protection against CCl 4-induced total protein, superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx) and glutathione S-transferase (GST) depletion in the liver. The plant extract restored the activities of the marker enzymes to near normal. In vitro antioxidant assay showed that SBSJ inhibit superoxide anions and hydrogen peroxide radicals with IC 50 values of 13.11 and 30.04 µg/ml, respectively. The present study indicated that methanolic extract of S. jollyanum possessed hepatoprotective activity against CCl 4-induced liver damage and this effect may be due to its strong antioxidant property.

African natural products with potential antioxidants and hepatoprotectives properties: a review

“…Tulbaghia violacea (Alliaceae) is a fast-growing, bulbous plant that reaches a height of 0.5 m. In the Eastern Cape of South Africa rhizomes of Tulbaghia violacea has been used for the treatment of jaundice, gall bladder stones, liver diseases and heart disease [96]. The rhizomes extract of T. violacea dose dependently attenuated atherosclerogenic induced alteration in markers of endothelial dysfunction, lipid profile, liver enzymes and histological changes [97].…”

The Potential Effect of Special Formulas on Cirrhotic Rats

“…The CCl 4 has been widely used in the induction of acute liver damage in experiment of mice model [5,6]. According to the previous studies, CCl 4 assumed to be a typical poison causing oxidative stress.…”

The protective role of 3-aminothiazolo[3-2a]benzimadzole-2-carbonitrile against lung and colon injury induced by nitrosomorpholine in adult male albino rat

“…In consistence, 5-nitro-2-(phenoxymethyl) benzimidazole significantly inhibited LPO, which was similar to that observed with butylated hydroxytoluene (BHT), a wellknown antioxidant. This action was returned to the O 2 -scavenging activity of the synthesized compounds at different concentration (Olorunisola et al, 2011). In addition, synthesized benzimidazole depending on the tissue have variable effects on cytochrome P 450 system which is also known to be expressed and regulated differentially in various tissues by numerous xenobiotics (Iscan et al, 1989).…”