Hepatoprotective and Antioxidant Effect ofBauhinia hookeriExtract against Carbon Tetrachloride-Induced Hepatotoxicity in Mice and Characterization of Its Bioactive Compounds by HPLC-PDA-ESI-MS/MS

Abstract

The hepatoprotective and antioxidant activity of Bauhinia hookeri ethanol extract (BHE) against CCl4-induced liver injury was investigated in mice. BHE was administered (500 and 1000 mg/kg/day) along with CCl4 for 6 weeks. The hepatic marker enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were determined in the serum. The antioxidant parameters: glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), and malondialdehyde (MDA) were estimated in the liver homogenate. BHE treatment significantly inhibited the CCl4-induced increase in ALT (44 and 64%), AST (36 and 46%), ALP (28 and 42%), and MDA (39 and 51%) levels at the tested doses, respectively. Moreover, BHE treatment markedly increased the activity of antioxidant parameters GSH, GPx, GR, GST, and SOD. Histological observations confirmed the strong hepatoprotective activity. These results suggest that a dietary supplement of BHE could exert a beneficial effect against oxidative stress and various liver diseases by enhancing the antioxidant defense status, reducing lipid peroxidation, and protecting against the pathological changes of the liver. The hepatoprotective activity of BHE is mediated, at least in part, by the antioxidant effect of its constituents. The active constituents of BHE were identified by HPLC-PDA-ESI/MS/MS.

Protective Effect of Astragalus polysaccharides on Liver Injury Induced by Several Different Chemotherapeutics in Mice

“…In our experimental models, DTX, CTX and EPI all caused liver injury, and high dose CTX (20 mg/kg) exhibited to have the most obvious toxicity, compared with other groups. The hepatotoxicity mainly manifested as serum enzyme levels Protective Effect of Astragalus Polysaccharideson Liver Injury Induced with Different Chemotherapeutics in Mice including aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increasing in the biochemical aspect, likewise methane dicarboxylic aldehyde (MDA) and superoxide dismutase (SOD) levels (Horton and Fairhurst, 1987;Al-Sayed et al, 2014). Histological changes including liver congestion, disorganization of hepatic cords and ballooning degeneration could be observed in H&E slides.…”

Synergistic protective role of ceftriaxone and ascorbic acid against subacute diazinon-induced nephrotoxicity in rats

“…Reactive oxygen species (ROS) are continuously generated inside the mammalian body as a result of exposure to many exogenous chemicals, xenobiotics and drugs in our environment and/or endogenous metabolic events involving electron transport mechanism and redox enzymes (Al-Sayed et al 2014;Eldahshan and Abdel-Daim 2014;Abdel-Daim 2014). In normal conditions, there is balance between the ROS produced and the antioxidants, as the ROS generated are neutralized by the endogenous antioxidants (Sun 1990).…”

Hepato-protective activity of three different extracts of Butea monosperma in CCl4 and Isoniazid induced toxicity in wistar rats

“…Induction of oxidative injury with the CCl 4 treatment has widely used and already been reported earlier [6,[8][9][10][11][12]. In biological system CCl 4 get converted into trichloromethyl free radical by cytochrome P450 system, which is a highly reactive free radical species that result lipid peroxidation of membranes.…”

“…Thousands of herbs have been reported to have antioxidative property [6,[10][11][12]. BM is known source of potent antioxidants such as quercetin, flavianoids, tannins, triterpenoid, gallic acid, ellagic acid, resin etc.…”