Hepatoprotective activity of praecoxin A isolated from Melaleuca ericifolia against carbon tetrachloride‐induced hepatotoxicity in mice. Impact on oxidative stress, inflammation, and apoptosis

Abstract

The hepatoprotective activity of praecoxin A, an ellagitannin from Melaleuca ericifolia, was determined against CCl 4 -induced toxicity in mice. Praecoxin A was administered (25, 50, and 100 mg/kg) for 5 days followed by CCl 4 . Praecoxin A markedly ameliorated the CCl 4 -induced increase in AST (by 19, 52, and 56%), ALP (22, 45, and 48%), ALT (11, 47, and 54%), total bilirubin (14, 27, and 28%), and MDA (26, 44, and 51%) at the tested doses, respectively, as compared with CCl 4 group. It was evident that praecoxin A significantly (p < 0.001) increased the antioxidant parameters GSH (45, 99, and 137%) and SOD (61, 129, and 159%). Histological findings revealed a marked amelioration of hepatocyte degeneration, necrosis, inflammatory cell infiltration, and hemorrhage in the groups treated with praecoxin A. COX-2 and caspase-3 hepatic expressions were significantly downregulated (p < 0.001) in praecoxin A-treated groups (up to 57, 83, and 93% for COX-2 and by 30, 82, and 99% for caspase-3). These findings suggest that praecoxin A exerts a beneficial effect against oxidative stress by reducing lipid peroxidation, enhancing the antioxidant defense status, and protecting against the histopathological changes induced by CCl 4 . This study highlights a promising natural hepatoprotective candidate derived from M. ericifolia that might be an alternative to silymarin.

Effects of infliximab against carbon tetrachloride-induced intestinal injury via lipid peroxidation and apoptosis

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Evaluation of neurotoxicity and hepatotoxicity effects of acute and sub-acute oral administration of unripe ackee (Blighia sapida) fruit extract

“…Reduction of non-enzymatic antioxidant GSH in neuronal and hepatic cells following sub-acute oral administration of unripe fruit extract of Blighia sapida could be a consequence of increased utilization for trapping free radicals. Depletion of GSH in the liver correspondingly affects the glutathione system in interrupting lipid peroxidation chain reaction [58,59]. The result from this study thereby establishes that unripe Blighia sapida fruit induces oxidative stress.…”

Hepatoprotective effects of Niudali (Callerya speciosa) root aqueous extracts against tetrachloromethane‐induced acute liver injury and inflammation

“…Acute liver injury induced by CCl 4 is associated with the activation of inflammatory processes, oxidative stress, and apoptosis (Al‐Sayed et al, 2019 ; Sun et al, 2022 ). In general, treatment with NWE, NPE, and hypaphorine all showed liver protective effects, as evidenced by data from the histopathological evaluation (liver injury score) and the blood chemical assays (ALT and AST level) (Table 2 ).…”