Hepatoprotective activity of melittin on isoniazid- and rifampicin-induced liver injuries in male albino rats

Abstract

Background The present study investigated the ameliorative effect of melittin, a major polypeptide in the venom of honeybee (Apis mellifera), on isoniazid-(INH) and rifampicin-(RIF) induced hepatotoxicity in male albino rats. Method Thirty rats (140-200 g) were divided into five groups (n = 6): normal control (NC) received normal saline orally (NaCl, 0.9%; toxic (T) group received INH + RIF (each rat received 100 mg/kg, p.o.); melittin (Mel15, Mel30) groups (each rat received 15 or 30 μg/kg s.c); and normal recovery (NR) group received INH + RIF (each rat received 100 mg/kg, p.o.). Blood and liver samples were collected for biochemical, hematological and histopathological studies respectively. Results The administration of melittin was found to prevent the antitubercular drug-induced alterations in the diagnostic markers; reduced glutathione (GSH), direct bilirubin (DB), total bilirubin (TB), aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), and total serum protein (TSP). Besides, hematological alterations were significantly high in Mel groups when compared to the toxic group. The NR group exhibited lower levels of DB, TB, ALP, LDH and TSP. In addition, treatment with melittin offered protection in the NR group with respect to MDA levels. Conclusion Evidence from this study suggests that melittin is beneficial for the prevention of acute hepatic failure in antitubercular drug-induced hepatoxicity and could be used as a potential therapeutic agent.

Hepatoprotective effect of hydromethanol extract of Otostegia integrifolia benth leaves in isoniazid and rifampicin induced Swiss albino mice

“…In this study serum bilirubin levels in drug only induced mice showed a significant (P < 0.01) increase as compared with a normal control group of mice. This study result is in agreement with the data of other authors showing that RIF produced competition for the elimination of bilirubin by the liver [ 49 ] and a combination of INH and RIF resulted in a higher rate of inhibition hepatic clearance of bile, an increase in lipid peroxidation in the hepatocyte, and cytochrome P450 involved in the synergistic effect of RIF and INH [ 30 , 50 , 51 ]. Whereas O. integrifolia leaves extract induced mice challenged with INH and RIF (G IV) did not show a significant difference as compared with the normal control group.…”

Pharmacological effects and mechanisms of bee venom and its main components: Recent progress and perspective

“…The above results suggested that BV is a promising alternative treatment option for non-alcoholic fatty liver disease ( Hanafi et al, 2018 ). BV and honey showed protective effects on hepatoxicity and lipid peroxidation in rats caused by LPS and carbon tetrachloride, while MEL possessed hepatoprotective activity on isoniazid- and rifampicin-induced liver hepatoxicity in rats, both of which suggested that a combination of BV and honey, or MEL could be beneficial for the prevention of hepatoxicity and could be used as potential therapeutic agents ( Meligi et al, 2020 ; Naji et al, 2021 ). Besides, after treatment with bvPLA 2 , the levels of cholestasis markers in the serum were attenuated, the abnormal state of liver fibrosis was restored, and hepatocyte inflammation and apoptosis were inhibited in mice with diet feeding 3,5-diethoxycarbonyl-1,4-dihydrocollidine ( Kim J.-Y.…”

Multiple approach of natural hepato-protectant against chemical toxicity

“…in wistar rats [9]. Naji et al attained hepatoprotective activity of velittin on isoniazed and rifampicin induced liver injury in male albino rats [61]. Farid et al deliberated anti inflammatory, antioxidant and hepatoprotective effects of Lactoferrin in rats [25].…”