Hepatoprotective Activity of Liposomal Flavonoid against Arsenite-Induced Liver Fibrosis

Abstract

Arsenic, the environmental metalloid toxicant, is known to induce oxidative damage to liver and produce hepatic fibrosis. The theme of our study was to optimize and evaluate the therapeutic efficacy of galactosylated liposomal flavonoidal antioxidant, quercetin (QC), in combating arsenic-induced hepatic fibrogenesis. The rats of the hepatic damage group were injected s.c. a single dose of sodium arsenite (NaAsO 2 ) (100.06 M/kg b. wt. in 0.5 ml of physiological saline). Hepatocytes and stellate cells were separated. Mitochondrial membranes were isolated from all those separated cells. Oxidative damage was monitored at different isolated subcellular parts of different hepatic cells. Liver fibrosis was also induced by the injection of NaAsO 2 . Galactosylated liposomal QC injection before NaAsO 2 treatment checked fibrogenesis completely by protecting the liver from oxidative attack in cellular and subcellular levels. The maximal protections from hepatocellular and fatty metamorphosis, necrosis, Kupffer cell hyperplasia, fibrosis, and in the deposition of collagen contents were observed and reconfirmed by our histopathological and histochemical analysis when rats were treated with galactosylated liposomal QC before NaAsO 2 injection. Application of galactosylated liposomal QC may be a potent therapeutic approach for NaAsO 2 -induced fibrogenesis through a complete protection against oxidative attack in cellular and subcellular parts of rat liver.

Hepatoprotective Effect of Quercetin Pretreatment Against Paracetamol-Induced Liver Damage and Partial Hepatectomy in Rats

“…was used as mobile-phase solvent and elution flow rate was 1 ml/min. The chromatographic peaks obtained were quantified by spectrophotometry (370 nm) 14 . The analytical curve was constructed by analyzing the following standards of quercetin: 0.001 mg/ml, 0.002 mg/ml, 0.006 mg/ml, 0.01 mg/ml and 0.02 mg/ml.…”

Hepatoprotective and neuroprotective activity of liposomal quercetin in combating chronic arsenic induced oxidative damage in liver and brain of rats

“…During chronic arsenic exposure, arsenic gains access into the cell and it becomes mandatory for a drug to cross cell membrane to chelate the intracellular arsenic. Hepatoprotective effect of a single injection of galactosylated liposomal quercetin on acute arsenite treated rats has been already studied by us in our previous observation (Mandal et al 2007). The same observation was made by Datta et al, 2003.…”

Sustained Liver Targeting and Improved Antiproliferative Effect of Doxorubicin Liposomes Modified with Galactosylated Lipid and PEG-Lipid

“…Many studies, especially those carried out by Hashida's group, verified that liposomes modified with galactosylated lipid achieved effective target to hepatocytes (3)(4)(5)(6). Our group has been dedicated to the studies on the targeted drug delivery of galactosylated liposomes in recently years.…”