Hepatoprotective activity of ethanolic extract of Salix subserrata against CCl4-induced chronic hepatotoxicity in rats

Abstract

BackgroundThe liver performs diverse functions that are essential for life. In the absence of reliable liver protective drugs, a large number of natural medicinal preparations are used for the treatment of liver diseases. Therefore the present study aims to investigate the hepatoprotective effects of Salix subserrata Willd flower ethanolic extract (SFEE) against carbon tetrachloride (CCl4)-induced liver damage.MethodsRats were divided into 4 groups of 10 animals each. Group I served as the normal healthy control, groups II rats were intoxicated with CCl4 i.p. (0.8 ml/kg body weight CCl4/olive oil, twice weekly for 9 weeks), group III rats received CCl4 i.p. and SFEE orally (150 mg/kg daily) and group IV rats received CCl4 i.p. and Silymarin orally (100 mg/kg, daily). The hepatoprotective potential of SFEE in rats was evaluated by measuring the protein levels of two inflammatory biomarkers; tumor necrosis factor-alpha (TNF-α) and nuclear factor kappa-B (NF-kB) in addition to other liver biomarkers. Histopathological changes in the liver were assessed using hematoxylin and eosin staining (HE).ResultsThe administration of SFEE showed hepatic protection at an oral dose of 150 mg/kg. SFEE significantly reduced the elevated serum levels of intracellular liver enzymes as well as liver biomarkers in comparison to CCl4− intoxicated group. Notably, SFEE significantly reduced the expression levels of TNF-α and NFkB proteins compared to their levels in CCl4 intoxicated group. These findings were confirmed with the histopathological observations, where SFEE was capable of reversing the toxic effects of CCl4 on liver cells compared to that observed in CCl4-intoxicated animals.ConclusionOur results show that SFEE has potential hepatoprotective effects at 150 mg/kg. These effects can be regarded to the antioxidant and anti-inflammatory properties of the extract.

Therapeutic potential of oleic acid nanovesicles prepared from petroleum ether extract of <i>Sargassum binderi</i> in streptozotocin–induced diabetic wound in Wistar rats

“…The cytokine TNF-α, a low molecular weight protein derived mainly from macrophages, is involved in inflammatory mechanism and closely associated with insulin resistance [17]. It is an inflammatory biomarker that plays a role in regulating the inflammatory processes in diabetes [18]. Consistent with the earlier report, the present study showed that STZ-induced diabetes was accompanied with significant increases in serum TNF-α level.…”

Therapeutic Effect of Milk Thistle (Silybum Marianum L) Seeds on Carbon Tetrachloride - Induced Hepatotoxicity in Rats

“…Also, these results are matched with Lien et al (2016). Moreover, Wahid et al (2016) demonstrated the effect of ethanolic extract of Salix subserrata flower against carbon tetrachloride -induced liver damage. This treatment was significantly (P < 0.001) capable of preventing the elevation in serum levels of liver enzymes and bilirubin.…”

Protective Effect of Traditional Medicine Tagtaggunsel on Carbon Tetrachloride Induced Chronic Liver Cirrhosis in a Rat Model

“…Group III were (TTG-treated) rats received the same dose of CCl4 as group II along with a daily oral dose of TTG (100 mg/kg). Group IV: (Silymarin-treated) rats received the same dose of CCl4 as group II along with a daily oral dose silymarin 14 (100 mg/kg). In groups III and IV, the treatment with TTG or silymarin was initiated 24 h after the first dose of CCl4.…”

“…The classic toxicity of carbon tetrachloride (CCl4) is to induce liver lesion and liver fibrosis 5 . Animal models using CC14 to induce chronic cirrhosis have been developed to study the effect of medicines on chronic cirrhosis 13,14 . In the present study, we aim to investigate the hepatoprotective effects of water extract of TTG against CCl4-induced liver fibrosis and its role in the alleviation of iron metabolism and restoration liver enzymes activities.…”